2020
DOI: 10.3390/plants9101295
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Biochemical and Molecular Investigation of In Vitro Antioxidant and Anticancer Activity Spectrum of Crude Extracts of Willow Leaves Salix safsaf

Abstract: Organic fractions and extracts of willow (Salix safsaf) leaves, produced by sequential solvent extraction as well as infusion and decoction, exhibited anticancer potencies in four cancerous cell lines, including breast (MCF-7), colorectal (HCT-116), cervical (HeLa) and liver (HepG2). Results of the MTT assay revealed that chloroform (CHCl3) and ethyl acetate (EtOAc)-soluble fractions exhibited specific anticancer activities as marginal toxicities were observed against two non-cancerous control cell lines (BJ-1… Show more

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Cited by 19 publications
(30 citation statements)
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“…For the cytotoxicity assessment, cells were trypsinized (Trypsin 0.05%/0.53 mM EDTA). Then, cells were seeded (2 × 10 3 cell/well in 100 μl of medium) into 96-well plates (Gibco, USA) for 24 h before treated with different concentrations of MeCc (2.5, 5, 7.5, 10, 15, 20 and μg/ml) for another 24 h ( Aboul-Soud et al, 2020 , Abo-Salem et al, 2020 ). To analyze the gene expression level and western blot of pro- and anti-apoptotic markers (p53, Bax, Caspase 3 and Bcl-2) , HepG-2 and BJ-1 cells were seeded (6 × 10 6 cell/well in 3 ml of medium) into in a 6-well plate.…”
Section: Methodsmentioning
confidence: 99%
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“…For the cytotoxicity assessment, cells were trypsinized (Trypsin 0.05%/0.53 mM EDTA). Then, cells were seeded (2 × 10 3 cell/well in 100 μl of medium) into 96-well plates (Gibco, USA) for 24 h before treated with different concentrations of MeCc (2.5, 5, 7.5, 10, 15, 20 and μg/ml) for another 24 h ( Aboul-Soud et al, 2020 , Abo-Salem et al, 2020 ). To analyze the gene expression level and western blot of pro- and anti-apoptotic markers (p53, Bax, Caspase 3 and Bcl-2) , HepG-2 and BJ-1 cells were seeded (6 × 10 6 cell/well in 3 ml of medium) into in a 6-well plate.…”
Section: Methodsmentioning
confidence: 99%
“…After 24 h of incubation with different MeCc concentrations, the media were discarded, and adherent cells were incubated with 100 µl/well MTT at a concentration of 0.5 mg/ml prepared in PBS and subsequently incubated at 37 °C for additional 3 h at 37 °C under dark condition ( Aboul-Soud et al, 2020 , Mosmann, 1983 ). Then, 100 μl isopropyl alcohol was added per well in order to dissolve the purple formazan crystals by the help of shaking for another 2 h at room temperature.…”
Section: Methodsmentioning
confidence: 99%
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“…Cell lines were cultured in DMEM/high glucose media supplemented with 2 mM L-glutamine, 10% FBS and 1% penicillin/streptomycin kept in Corning ® 75cm² U-Shaped canted neck cell culture flask with vent cap (Corning, New York, USA). Then, sub-confluent cultures (70-80%) were trypsinized (Trypsin 0.05%/0.53 mM EDTA) and spilt depending on the seeding ratio [26,27].…”
Section: In Vitro Cytotoxicitymentioning
confidence: 99%