Biochemical and molecular identification of Solanum lycopersicum L. temperature tolerant bacterial endophytes

Singh, Monika; Kumar, Ajay; Pandey, Kapil Deo

doi:10.1016/j.bcab.2019.101409

Cited by 9 publications

(9 citation statements)

References 51 publications

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“…A subset of bacterial endophytes isolated from tomato endorhizosphere (77 isolates), identified by partial sequencing of their 16S rRNA gene, belonged to two phyla (Firmicutes and Proteobacteria) and to three orders, namely, Bacillales (27.3%), with all the isolates in the genus Bacillus; Pseudomonadales (31.2%), with isolates in the genera Pseudomonas and Acinetobacter; and Enterobacteriales (41.6%), with isolates in the genera Enterobacter, Ewingella, Pantoea, Providencia, and Lelliottia. Similarly, some of these genera (Bacillus, Pseudomonas, Acinetobacter, Enterobacter) have been isolated from tomato endorhizosphere in studies on beneficial bacteria (Tian et al, 2017;Singh et al, 2019). Bacterial strains in the genera, Rhizobium and Ralstonia, have also been isolated from the endorhizophere of tomato plants (Abbamondi et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Beneficial Bacteria Traits Associated With Tomato Root in Greenhouse Environment Reveals That Sampling Sites Impact More Than the Root Compartment

et al. 2021

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Tomato is subject to several diseases that affect both field- and greenhouse-grown crops. To select cost-effective potential biocontrol agents, we used laboratory throughput screening to identify bacterial strains with versatile characteristics suitable for multipurpose uses. The natural diversity of tomato root–associated bacterial communities was bioprospected under a real-world environment represented by an intensive tomato cultivation area characterized by extraseasonal productions in the greenhouse. Approximately 400 tomato root–associated bacterial isolates, in majority Gram-negative bacteria, were isolated from three compartments: the soil close to the root surface (rhizosphere, R), the root surface (rhizoplane, RP), and the root interior (endorhizosphere, E). A total of 33% of the isolates produced siderophores and were able to solubilize phosphates and grow on NA with 8% NaCl. A total of 30% of the root-associated bacteria showed antagonistic activity against all the tomato pathogens tested, i.e., Clavibacter michiganesis pv. michiganensis, Pseudomonas syringae pv. tomato, Pseudomonas corrugata and Xanthomonas euvesicatoria pv. perforans, and Fusarium oxysporum f. sp. lycopersici. We found that the sampling site rather than the root compartment of isolation influenced bacterial composition in terms of analyzed phenotype. This was demonstrated through a diversity analysis including general characteristics and PGPR traits, as well as biocontrol activity in vitro. Analysis of 16S rRNA gene (rDNA) sequencing of 77 culturable endophytic bacteria that shared multiple beneficial activity revealed a predominance of bacteria in Bacillales, Enterobacteriales, and Pseudomonadales. Their in vitro antagonistic activity showed that Bacillus species were significantly more active than the isolates in the other taxonomic group. In planta activity against phytopathogenic bacteria of a subset of Bacillus and Pseudomonas isolates was also assessed.

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Section: Discussionmentioning

confidence: 99%

Bioprospecting of Beneficial Bacteria Traits Associated With Tomato Root in Greenhouse Environment Reveals That Sampling Sites Impact More Than the Root Compartment

et al. 2021

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“…86.95 µg ml −1 ) as determined by the Salkowski assay (Table 1 ) and is comparable to that in the AP strain MB-17a (reportedly producing 110.5 µg/ml IAA) 33 . Other reported AP strains such as YP3, YP4 and IC59 produced low IAA (59.2, 43.8, and 21.9 µg ml −1 , respectively) 34 , 40 , and few others were reported merely as positive for this PGP trait 38 , 40 , 43 .…”

Section: Resultsmentioning

confidence: 93%

“…The highest solubilization potential (17.6 µg ml −1 ) could be achieved within 48 h in liquid NBRIP medium. Like JAS1, another AP strain MB-17a isolated from mung bean roots is also reported with slightly higher phosphate solubilization (PSI of 2.67 cm and solubilization potential of up to 53 μg ml −1 on the 6th day) 33 , while two other studied isolates from soybean and tomato are reported negative for phosphate solubilization potential 38 , 43 .…”

Section: Resultsmentioning

confidence: 96%

“…JAS1 showed a peculiar yellow halo zone around its streaks over CAS agar inferring a positive siderophore production profile, quantifiably of 30.1% (%SU) at the 120 th hour of growth (Table 1 ). Previously, the IC59 strain of AP was reported with siderophore production of up to 30.6% units 34 while few others viz., YP3, YP4, MB-17a, and MS-1 strains were reported positive for siderophores 40 , 43 .…”

Section: Resultsmentioning

confidence: 99%

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An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention

Kaur

Mudgal

Chand

et al. 2022

Sci Rep

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A peculiar bacterial growth was very often noticed in leaf-initiated tissue cultures of Sansevieriatrifasciata, a succulent belonging to the Asparagaceae family. The isolate left trails of some highly viscous material on the walls of the suspension vessels or developed a thick overlay on semisolid media without adversities in plant growth. FTIR identified this substance to be an extracellular polysaccharide. Various morphological, biochemical tests, and molecular analyses using 16S rRNA, atpD, and recA genes characterized this isolate JAS1 as a novel strain of Agrobacteriumpusense. Its mucoidal growth over Murashige and Skoog media yielded enormous exopolysaccharide (7252 mg l−1), while in nutrient agar it only developed fast-growing swarms. As a qualifying plant growth-promoting bacteria, it produces significant indole-3-acetic acid (86.95 mg l−1), gibberellic acid (172.98 mg l−1), ammonia (42.66 µmol ml−1). Besides, it produces siderophores, 1-aminocyclopropane-1-carboxylicaciddeaminase, fixes nitrogen, forms biofilms, and productively solubilizes soil inorganic phosphates, and zinc. Under various treatments with JAS1, wheat and chickpea resulted in significantly enhanced shoot and root growth parameters. PGP effects of JAS1 positively enhanced plants’ physiological growth parameters reflecting significant increments in overall chlorophyll, carotenoids, proline, phenols, flavonoids, and sugar contents. In addition, the isolated strain maintained both plant and soil health under an intermittent soil drying regime, probably by both its PGP and EPS production attributes, respectively.

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“…Rhizobium pusense MS-1 (Accession number: MF977751), Bacillus cereus MS-2 (Accession number: KY018604), B. flexus MS-3 Accession number: KX858538), Methylophilus flavus MS-4 (Accession number: MF977752) and Pseudomonas aeruginosa MS-5 (Accession number: KX817189, which were previously isolated from the roots of tomatoes, were stored in the Laboratory of Environmental Microbiology, Department of Botany, B.H.U. [ 25 ]. The bacterial strains were grown to the mid-log phase, and an adjusted cell biomass (~10 9 cells ml −1 ) was harvested using centrifugation (4000 rpm, 5 min at 4 °C), and suspended in phosphate buffer saline (PBS, pH-7.5 10 −2 M).…”

Section: Methodsmentioning

confidence: 99%

Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis

Singh

Qureshi

Jaremko

et al. 2022

Plants

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In this study, five endophytic bacterial strains, namely Rhizobium pusense (MS-1), Bacillus cereus MS-2, Bacillus flexus (MS-3), Methylophilus flavus (MS-4), and Pseudomonas aeruginosa (MS-5), were used to investigate their potential role in the enhancement of growth yields of two types of tomato varieties, viz. hybrid and local, and in the biosynthesis of silver nanoparticles (AgNPs). The inoculation of bacterial strains enhanced the root and shoot length, biomass, and leaf chlorophyll contents. The fruit weight of the tomato (kg/plant) was also higher in the bacteria inoculated plants of both hybrid and local varieties than in the control (untreated). A significant increase was recorded in the fruit yield (g/plant) in all the treatments, whereas Methylophilus flavus (MS-4) inoculated plants yielded nearly 2.5 times more fruit weight compared to the control in the hybrid variety and two times higher in the local variety. The response to M. flavus as a microbial inoculant was greater than to the other strains. Biosynthesis of Ag nanoparticles was also carried out using all five endophytic bacterial strains. The weakest producers of AgNPs were Rhizobium pusense (MS-1) and Methylophilus flavus (MS-4), while Bacillus cereus MS-2, Bacillus flexus (MS-3), and Pseudomonas aeruginosa (MS-5) were strong producers of AgNPs. Nanoparticles were further characterized using high-resolution scanning electron microscopy (HR-SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV-Vis spectrophotometry, and X-ray diffraction (XRD) analysis, and revealed cuboidal shaped AgNPs in the Bacillus cereus MS-2 strain. In addition, the biosynthesized AgNPs showed antibacterial activity against various pathogenic and endophytic bacterial strains.

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Biochemical and molecular identification of Solanum lycopersicum L. temperature tolerant bacterial endophytes

Cited by 9 publications

References 51 publications

Bioprospecting of Beneficial Bacteria Traits Associated With Tomato Root in Greenhouse Environment Reveals That Sampling Sites Impact More Than the Root Compartment

Bioprospecting of Beneficial Bacteria Traits Associated With Tomato Root in Greenhouse Environment Reveals That Sampling Sites Impact More Than the Root Compartment

An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention

Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis

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