BIOCHEMICAL AND MOLECULAR IDENTIFICATION OF SOME SOYBEAN (Glycine max L.) CULTIVARS

Abstract

This study was carried out to distinguish among eight soybean (Glycine max L.) cultivars using biochemical and molecular marker. Therefore, SDS-storage proteins, some isozyme (esterase (EST), peroxidase (Prx) and glutamate oxaloacetate transaminase (GOT)) and PCR-RAPD (polymerase chain reactionrandomly amplified polymorphic DNA) markers were carried out. The results of protein electrophoresis revealed that total number of bands ranged from 10 in cultivar G.21 to 14 in cultivar Crawford.. Nine common bands were found in all cultivars. Some cultivars showed specific bands which could be used to distinguish between others.