Biochemical and molecular evidence on the role of vaspin in early detection of the insulin resistance in a rat model of high-fat diet and use of diazinon

Maghsoudi, Armin Salek; Hassani, Shokoufeh; Momtaz, Saeideh; Shadboorestan, Amir; Ganjali, Mohammad Reza; Ghahremani, Mohammad Hossein; Hosseini, Reshad; Norouzi, Parviz; Abdollahi, Mohammad

doi:10.1016/j.tox.2018.10.014

Cited by 38 publications

(26 citation statements)

References 103 publications

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“…Plasma samples were collected from high fat diet-induced diabetic rats according to previous study protocol. 4 All the ethical issues were cautiously adhered. The experiments were carried out in full accordance with the Declaration of Helsinki and national guidelines for the use and care of laboratory animals.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…Several studies have shown that plasma or serum serpin A12 concentration rises in conditions of obesity and insulin resistance. [2][3][4] In previous studies, mean plasma serpin A12 concentrations have been reported ranging from 0.1-7 ng/mL [5][6][7] with increases during the early stages of insulin resistance. 4 Currently, antibody-based ELISA methods are available for the analysis of serpin A12.…”

Section: Introductionmentioning

confidence: 96%

“…[2][3][4] In previous studies, mean plasma serpin A12 concentrations have been reported ranging from 0.1-7 ng/mL [5][6][7] with increases during the early stages of insulin resistance. 4 Currently, antibody-based ELISA methods are available for the analysis of serpin A12. 8,9 The ELISA technique, although sensitive, has some disadvantages like high cost dependent on the prepared kits and experimental complexities.…”

Section: Introductionmentioning

confidence: 96%

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<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

Maghsoudi¹,

Hassani²,

Akmal³

et al. 2020

IJN

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In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs. Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K 3 [Fe (CN) 6 ]/K 4 [Fe (CN) 6 ] solution (redox probe). Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039-10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively. Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.

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Section: Experimental Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

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<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

Maghsoudi¹,

Hassani²,

Akmal³

et al. 2020

IJN

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“…42 In addition, another study by 43 showed that ROS production induced apoptosis in human T-cells and monocytes. [43][44][45] Furthermore, production of ROPS, such as: superoxide anion (O 2 − ), hydrogen peroxide, hydroxyl radicals, and generation of nitric oxide free radicals found to be associated with pancreatic cells dysfunction or cell death. This action is induced by autoimmune response and abnormal actions of cytokines in diabetes.…”

mentioning

confidence: 99%

Biochemical evidence on the potential role of methyl mercury in hepatic glucose metabolism through inflammatory signaling and free radical pathways

Maqbool

Bahadar

Hassani

et al. 2019

J of Cellular Biochemistry

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Methylmercury (MeHg) is an extremely important environmental toxicant posing serious health risks to human health and a big source of environmental pollutant. Numerous evidence available showing a link between nervous system toxicity and MeHg exposure. Other forms of mercury are reason of metabolic toxic effects and alteration of DNA in the human body. The sources of exposure could be occupational or other environmental settings. In the present study MeHg was orally gavaged to mice, at doses of 2.5, 5, and 10 mg/kg for 4 weeks. Fasting hyperglycemia, activity of hepatic phoshphoenolpyruvate carboxykinase and glucose 6‐phoshphate were reported high as compared to control group. Inflammatory markers like, tumor necrosis factor α, the actual end product of inflammatory mediators’ cascade pathway was also raised in comparison to control group. Hyperinsulinemia observed in serum showed clear understanding of mercury induced insulin resistance. Moreover, tissue damage due to increased oxidative stress markers like, hepatic lipid peroxidation, 8‐deoxygunosine, reactive oxygen species, and carbonyl groups was significantly higher as compared to control group. MeHg caused a significant reduction in antioxidant markers like ferric reducing antioxidant power and total thiol molecules. The present study highlighted that activity of key enzymes involved in glucose metabolism is changed, owing to MeHg induced toxicity in the liver. Induction of similar toxic effects assumed to be stimulated by the production of high quantity free radicals.

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“…У тварин всіх груп визначали рівень TNF-α, окислювального стресу та васпіну. Вчені припустили у висновку, щодо можливого діагностично-надійного значення васпіну, як маркера ІР [43].…”

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The Value of the Tumor Necrosis Factor-Alpha and Vaspin Ratio in the Development of Insulin Resistance

Zhuravlyova

Pylov

2019

PEP

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Автори гарантують повну відповідальність за все, що опубліковано в статті. Автори гарантують відсутність конфлікту інтересів і власної фінансової зацікавленості при виконанні роботи та написанні статті. Рукопис надійшов до редакції 17.01.2019.

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Biochemical and molecular evidence on the role of vaspin in early detection of the insulin resistance in a rat model of high-fat diet and use of diazinon

Cited by 38 publications

References 103 publications

<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

Biochemical evidence on the potential role of methyl mercury in hepatic glucose metabolism through inflammatory signaling and free radical pathways

The Value of the Tumor Necrosis Factor-Alpha and Vaspin Ratio in the Development of Insulin Resistance

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