Biochemical and molecular characterization of the homocysteine S-methyltransferase from broccoli (Brassica oleracea var. italica)

Lyi, Sangbom M.; Zhou, Xin; Kochian, Leon V.; Li, Li

doi:10.1016/j.phytochem.2007.02.007

Cited by 44 publications

(31 citation statements)

References 19 publications

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“…Lyi et al ( 2005 ) reported that the key factor responsible for the SeMSC formation (the most effective anticarcinogenic Se compound) is the SMT enzyme. Lyi et al ( 2007 ) also observed that metabolism of S and Se in broccoli is controlled by the same enzymes and apparently, this similarity occurs in other plants (Kabata-Pendias 2011 ).…”

Section: Physiological Importance Of Selenium For Higher Plantsmentioning

confidence: 55%

Selenium and its Role in Higher Plants

El-Ramady¹,

Abdalla²,

Alshaal³

et al. 2015

Pollutants in Buildings, Water and Living Organisms

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Section: Physiological Importance Of Selenium For Higher Plantsmentioning

confidence: 55%

Selenium and its Role in Higher Plants

El-Ramady¹,

Abdalla²,

Alshaal³

et al. 2015

Pollutants in Buildings, Water and Living Organisms

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“…Realtime qRT-PCR was performed using SYBR Green Supermix (Bio-Rad) and gene-specific primers (Supplemental Table S2) in an ABI 7500 Real-Time PCR system (Applied Biosystems). The Arabidopsis ubiquitin extension protein1 gene was used as a nuclear gene internal control, and relative expression levels were calculated using the cycle threshold method (Lyi et al, 2007). Values reported represent the averages of three biological replicates with two technical trials.…”

Section: Rna Extraction and Qrt-pcrmentioning

confidence: 99%

A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis

et al. 2015

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“…Genespecific primers, which were confirmed to produce specific gene products by sequencing , are listed in Supplemental Table S1. The relative transcript levels were calculated as described previously (Lyi et al, 2007).…”

Section: Nucleic Acid Analysismentioning

confidence: 99%

The Purple Cauliflower Arises from Activation of a MYB Transcription Factor

Chiu

Zhou²,

Burke³

et al. 2010

Plant Physiology

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Anthocyanins are responsible for the color of many flowers, fruits, and vegetables. An interesting and unique Purple (Pr) gene mutation in cauliflower (Brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a few other tissues. To unravel the nature of the Pr mutation in cauliflower, we isolated the Pr gene via a combination of candidate gene analysis and fine mapping. Pr encoded a R2R3 MYB transcription factor that exhibited tissue-specific expression, consistent with an abnormal anthocyanin accumulation pattern in the mutant. Transgenic Arabidopsis (Arabidopsis thaliana) and cauliflower plants expressing the Pr-D allele recapitulated the mutant phenotype, confirming the isolation of the Pr gene. Up-regulation of Pr specifically activated a basic helix-loop-helix transcription factor and a subset of anthocyanin structural genes encoding flavonoid 3’-hydroxylase, dihydroflavonol 4-reductase, and leucoanthocyanidin dioxygenase to confer ectopic accumulation of pigments in the purple cauliflower. Our results indicate that the genetic variation including a Harbinger DNA transposon insertion in the upstream regulatory region of the Pr-D allele is responsible for the up-regulation of the Pr gene in inducing phenotypic change in the plant. The successful isolation of Pr provides important information on the regulatory control of anthocyanin biosynthesis in Brassica vegetables, and offers a genetic resource for development of new varieties with enhanced health-promoting properties and visual appeal.

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Biochemical and molecular characterization of the homocysteine S-methyltransferase from broccoli (Brassica oleracea var. italica)

Cited by 44 publications

References 19 publications

Selenium and its Role in Higher Plants

Selenium and its Role in Higher Plants

A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis

The Purple Cauliflower Arises from Activation of a MYB Transcription Factor

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