Biochemical and Immunological Analyses of the Flagellin of <i>Clostridium tyrobutyricum</i> ATCC 25755

Arnold, Françoise; Bédouet, Laurent; Batina, P.; Robreau, Georges; Talbot, F.; Lécher, Pierre; Malcoste, R.

doi:10.1111/j.1348-0421.1998.tb01965.x

Cited by 21 publications

(19 citation statements)

References 33 publications

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“…(3) The flagellin of C. difficile has been reported to be post-translationally modified (Tasteyre et al 2000), and the modification is likely to be glycan, however, with significantly fewer putative glycosylation genes associated with the flagellin genes. The modification probably differs from that of C. botulinum, C. tetani, and C. acetobutylicum; (4) Flagellin glycosylation has also been reported in Clostridium tyrobutyricum (Arnold et al 1998). …”

Section: Components Of the Cell Surfacementioning

confidence: 99%

Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes

Sebaihia¹,

Peck²,

Minton³

et al. 2007

Genome Res.

233

215

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Clostridium botulinum is a heterogeneous Gram-positive species that comprises four genetically and physiologically distinct groups of bacteria that share the ability to produce botulinum neurotoxin, the most poisonous toxin known to man, and the causative agent of botulism, a severe disease of humans and animals. We report here the complete genome sequence of a representative of Group I (proteolytic) C. botulinum (strain Hall A, ATCC 3502). The genome consists of a chromosome (3,886,916 bp) and a plasmid (16,344 bp), which carry 3650 and 19 predicted genes, respectively. Consistent with the proteolytic phenotype of this strain, the genome harbors a large number of genes encoding secreted proteases and enzymes involved in uptake and metabolism of amino acids. The genome also reveals a hitherto unknown ability of C. botulinum to degrade chitin. There is a significant lack of recently acquired DNA, indicating a stable genomic content, in strong contrast to the fluid genome of Clostridium difficile, which can form longer-term relationships with its host. Overall, the genome indicates that C. botulinum is adapted to a saprophytic lifestyle both in soil and aquatic environments. This pathogen relies on its toxin to rapidly kill a wide range of prey species, and to gain access to nutrient sources, it releases a large number of extracellular enzymes to soften and destroy rotting or decayed tissues.

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Section: Components Of the Cell Surfacementioning

confidence: 99%

Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes

Sebaihia¹,

Peck²,

Minton³

et al. 2007

Genome Res.

233

215

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“…The first reports of flagellin glycosylation in Clostridium species were for C. tyrobutyricum and C. acetobutylicum ATCC 824 (382)(383)(384). C. botulinum was the first human-pathogenic Clostridium species in which Oglycosylation of flagellin was studied.…”

Section: Protein Glycosylationmentioning

confidence: 99%

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Tytgat

Lebeer

2014

Microbiol Mol Biol Rev

128

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SUMMARY Humans have been increasingly recognized as being superorganisms, living in close contact with a microbiota on all their mucosal surfaces. However, most studies on the human microbiota have focused on gaining comprehensive insights into the composition of the microbiota under different health conditions (e.g., enterotypes), while there is also a need for detailed knowledge of the different molecules that mediate interactions with the host. Glycoconjugates are an interesting class of molecules for detailed studies, as they form a strain-specific barcode on the surface of bacteria, mediating specific interactions with the host. Strikingly, most glycoconjugates are synthesized by similar biosynthesis mechanisms. Bacteria can produce their major glycoconjugates by using a sequential or an en bloc mechanism, with both mechanistic options coexisting in many species for different macromolecules. In this review, these common themes are conceptualized and illustrated for all major classes of known bacterial glycoconjugates, with a special focus on the rather recently emergent field of glycosylated proteins. We describe the biosynthesis and importance of glycoconjugates in both pathogenic and beneficial bacteria and in both Gram-positive and -negative organisms. The focus lies on microorganisms important for human physiology. In addition, the potential for a better knowledge of bacterial glycoconjugates in the emerging field of glycoengineering and other perspectives is discussed.

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“…In contrast, Dons et al (11) determined that only a single flagellin gene was present within the genome and noted a discrepancy between the predicted and actual molecular weights of the L. monocytogenes flagellin. The flagellins from a number of gram-negative (5,14,15,21) and gram-positive (1,20) bacteria have previously been identified as glycosylated. Structural studies on both Campylobacter and Helicobacter flagella have shown that the flagellin structural proteins are posttranslationally modified at multiple sites with a novel sialic acid-like sugar, Pse5Ac7Ac, and related derivatives, via an O-linked glycosylation process (40,45).…”

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confidence: 99%

Flagellin fromListeria monocytogenesIs Glycosylated with β-O-LinkedN-Acetylglucosamine

et al. 2004

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Biochemical and Immunological Analyses of the Flagellin of Clostridium tyrobutyricum ATCC 25755

Abstract: Abstract:The monoclonal antibody 21E7-B12

Cited by 21 publications

References 33 publications

Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes

Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Flagellin fromListeria monocytogenesIs Glycosylated with β-O-LinkedN-Acetylglucosamine

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