Biochemical and immunocytochemical characterization of monoclonal antibody TOP 35 raised against purified tonoplast from cress root

Liedtke, Christa; Polsakiewicz, Monika; Hartmann, Ingrid; Peters, Petra; Volkmann, Dieter; Scherer, Gerd

doi:10.1093/jxb/48.6.1215

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…Furthermore, we confirmed by immunoblotting using anti TOP35 antibodies [34] that the tonoplast was depleted in this fraction (data not shown). Thus this protein is likely to be derived from a v-ATPase in transit to the vacuole or with a function in a nonvacuolar membrane.…”

Section: Identification Of Organelle Marker Proteinsmentioning

confidence: 51%

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A proteomic analysis of organelles fromArabidopsis thaliana

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Sherrier²,

Mahon³

et al. 2000

Electrophoresis

122

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We introduce the use of Arabidopsis thaliana callus culture as a system for proteomic analysis of plant organelles using liquid-grown callus. This callus is relatively homogeneous, reproducible and cytoplasmically rich, and provides organelles in sufficient quantities for proteomic studies. A database was generated of mitochondrial, endoplasmic reticulum (ER), Golgi/prevacuolar compartment and plasma membrane (PM) markers using two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2-D SDS-PAGE) and peptide sequencing or mass spectrometric methods. The major callus membrane-associated proteins were characterised as being integral or peripheral by Triton X-114 phase partitioning. The database was used to define specific proteins at the Arabidopsis callus plasma membrane. This database of organelle proteins provides the basis for future characterisation of the expression and localisation of novel plant proteins.

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Section: Identification Of Organelle Marker Proteinsmentioning

confidence: 51%

“…Protein concentration was assayed by the bicinchoninic acid (BCA) method (Pierce, Rockford, IL, USA). Western blot analysis was carried out as described in Wee et al [21] using Caxton V [32] to detect PM ATPase, 2E7 to detect v-ATPase subunit B [33], and a monoclonal antibody against TOP 35 [34].…”

Section: Biochemical Fractionation and Analysismentioning

confidence: 99%