Biochemical and hormonal analysis of follicular fluid and serum of female dromedary camels (Camelus dromedarius) with different sized ovarian follicles

El-Bahr, Sabry M.; Ghoneim, I.M.; Waheed, M.M.

doi:10.1016/j.anireprosci.2015.06.002

Cited by 15 publications

(12 citation statements)

References 46 publications

(69 reference statements)

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“…Our results are consistent with those of Khan et al [35] in buffaloes but differ from Probo et al [9] in cattle and Ghoneim et al [40] and El -Bahr et al [43] in camels. This divergence may be explained by the difference in breeding conditions, climatic factors, stage of lactation and food intake.…”

Section: Discussionsupporting

confidence: 92%

Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Mimoune¹,

Kaidi²,

Azzouz³

et al. 2017

Kafkas Univ Vet Fak Derg

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A clinical study was performed to evaluate the diagnostic methods for ovarian cysts (OC), and to determine the metabolic profiles of animals with OC in the region of Mitidja in the North of Algeria. A total of 504 non-pregnant lactating cows were used in this study. Ultrasonography was performed by EXAGO scanner and was combined with assessment of serum P4. Biochemical serum parameters were assayed by spectrophotometry and insulin and cortisol serum measurement was performed by electrochemiluminescence. The results showed an overall incidence of 11.9% of OC. The incidence of OC was higher among cows in third lactation. Holstein breed was the most affected by OC compared with other breeds (P<0.001). There were no effects of average BCS (Body Condition Scoring) and milk production on the incidence of OC (P>0.05). OC were single in 91% of cases. They were found mainly on the right ovary (66.66%). Seasonality had a significant influence on incidence rate of OC with higher incidence rates during winter and spring (71.66%); while, 28.33% of OC were detected during the summer and autumn (P<0.05). OC were associated with low serum concentrations of glucose, insulin and urea as well as high levels of cortisol. Ultrasound examination and progesterone assays were proposed as the most effective diagnostic combination to diagnose OC. In conclusion, in addition to hormonal imbalances, metabolic disorders are involved in the formation and/or persistence of OC. Therefore, the use of metabolic indicators in understanding and exploration of OC is of great interest. Keywords: Cow, Ovarian cyst, Metabolic profile, Ultrasonography Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma ÖzetOvaryum kistlerinin tanısında kullanılan metotları değerlendirmek ve Cazayir'in kuzeyindeki Mitidja bölgesindeki ovaryum kistli hayvanların metabolik profillerini belirlemek amacıyla klinik bir çalışma gerçekleştirildi. Toplam 504 gebe olmayan laktasyondaki inekler bu çalışmada kullanıldı. EXAGO tarayıcı kullanılarak ultrasonografi uygulandı ve serum P4 verileri ile birlikte değerlendirildi. Biyokimyasal serum parametreleri spektrofotometri ile belirlenirken insülin ve kortizol serum ölçümleri elektrokimyasal görüntüleme ile gerçekleştirildi. Ovaryum kist insidansı %11.9 olarak belirlendi. Ovaryum insidansı üçüncü laktasyondaki ineklerde daha fazla oranda tespit edildi. Diğer türler ile karşılaştırıldığında Holstein ırkında ovaryum kisti daha fazla oranda gözlemlendi (P<0.001). Ovaryum kisti üzerine Vücut Kondisyon Skorunun bir etkisi gözlemlenmedi (P>0.05). Ovaryum kisti, vakaların %91'inde tek taraflı olarak tespit edildi. Kistler çoğunlukla (%66.66) sağ ovaryumda yer almaktaydı. Mevsimin ovaryum kistlerinin ortaya çıkmasında önemli bir etkiye neden olduğu, kış ile ilkbahar aylarında (%71.66) insidansın arttığı yaz ve sonbaharda ise düşük seviyede (%28.33) olduğu belirlendi (P<0.05). Ovaryum kistlerinin düşük glukoz, insülin ve üre ile yüksek kortizol serum konsantrasyonları ile ilişkili olduğu belirlendi. Ult...

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Section: Discussionsupporting

confidence: 92%

Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Mimoune¹,

Kaidi²,

Azzouz³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…Aller et al (2) and Hudson et al (30) noted similar results. In camels, El-Bahr et al (22) reported that cholesterol levels in serum were 3.5 times higher than those of the follicular fluid. Thereafter, they suggested that blood cholesterol is not the major metabolite available for steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Thereafter, they suggested that blood cholesterol is not the major metabolite available for steroidogenesis. Indeed, the granulosa cells have a large stock of cholesterol esters that are capable of providing cholesterol to ovarian function (22,31). In addition, the cholesterol level is directly related to the energy state of animal.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Profile Comparison between Follicular Fluid and Serum in Normal Cows and Those Affected by Ovarian Cysts

Mimoune

Kaidi

Benaissa

et al. 2019

Macedonian Veterinary Review

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The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier.

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“…As such, the concentrations of the free IGF1 in the growing follicle might determine follicle fate. This may be explained by the results of El-Bahr et al, (2015) who demonstrated that IGF1 concentrations in medium to large follicles were found to be comparable to IGF1 serum concentration. Furthermore, IGF1 supports FSH and allows the future dominant follicle to quickly upregulate estradiol production and hence to suppress circulating FSH and prevent the subordinate follicles from further growth (Fortune et al, 2004).…”

Section: Discussionmentioning

confidence: 89%

Biochemical Compositions in the Follicular Fluid of Different Follicle Groups and Their Relationship with the Blood Concentrations in Dromedary Camels

Shehab-El-Deen¹,

Al-Dobaib²,

Al-Sobayil³

2019

IJB

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Metabolic profile changes of the follicular fluid of the growing follicles can be used as an indirect indicator of the oocyte and granulosa cells quality. The aim of this study was to investigate the biochemical compositions of follicular fluid collected from follicles at different stages of growth and their relationship with that of blood serum in Dromedary camel. Ovaries were colleceted from local slaughterhouse (Oniza, KSA). Soon after slaughtering, blood samples were collected from Dromedary camel (n = 20) and follicular fluid was aspirated from three different groups of non-atretic follicles (4–6)mm, 6–8mm and 10–20mm diameter). Follicular samples were pooled by maintaining the follicular sizes. Concentrations of glucose, cholesterol, triglycerides, urea, total protein, lactate dehydrosenase (LDH), cortisol, triiodothyronine (T3), insulin-like growth factor-1 (IGF-1) and non-esterified fatty acids (NEFA) were assayed in each serum and follicular fluid sample. The concentrations of glucose, cholesterol, triglycerides, total protein, LDH, T3, IGF-1 and NEFA decreased in the follicular fluid irrespective of follicular sizes and increasing in trend for urea in comparison with blood serum. There was a significant concentration gradient for IGF-1 in small follicular group compared to large or medium groups. Our data from the present study suggest that the oocyte and the granulosa cells of Dromedary she camel develop in a biochemical environment that does not have remarkable changes from small to large follicles except for IGF-1. In conclusion, the above-mentioned metabolic changes in the growing follicle is related to blood metabolic changes and, therefore, may be used in determining follicular dominance, oocyte and granulosa cells quality in Dromedary camel.

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Biochemical and hormonal analysis of follicular fluid and serum of female dromedary camels (Camelus dromedarius) with different sized ovarian follicles

Cited by 15 publications

References 46 publications

Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Sütçü İneklerde Ovaryum Kistlerinin Tanısı ve Metabolik Profili Üzerine Bir Çalışma

Metabolic Profile Comparison between Follicular Fluid and Serum in Normal Cows and Those Affected by Ovarian Cysts

Biochemical Compositions in the Follicular Fluid of Different Follicle Groups and Their Relationship with the Blood Concentrations in Dromedary Camels

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