Biochemical and histopathological analysis after sub-chronic administration of oxyresveratrol in Wistar rats

Alam, Nisat; Najnin, Hasina; Islam, Maidul; Shakya, Sonam; Khan, Ishaat M.; Zaidi, Rana

doi:10.1080/01480545.2021.2015243

Cited by 6 publications

(3 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A general toxicological evaluation, clinical pathological examination, and histopathological studies were conducted, following the reported protocols [6][7][8][9].…”

Section: Toxicological Evaluationmentioning

confidence: 99%

Sub-Chronic Toxicological Evaluation of the Sesquiterpene Lactone-Enriched Fraction of Tithonia diversifolia (Hemsley) A. Gray in Experimental Rats

Egbule,

Oji,

Nnadi

2023

Iect 2023

View full text Add to dashboard Cite

show abstract

“…A general toxicological evaluation, clinical pathological examination, and histopathological studies were conducted, following the reported protocols [6][7][8][9].…”

Section: Toxicological Evaluationmentioning

confidence: 99%

Sub-Chronic Toxicological Evaluation of the Sesquiterpene Lactone-Enriched Fraction of Tithonia diversifolia (Hemsley) A. Gray in Experimental Rats

Egbule,

Oji,

Nnadi

2023

Iect 2023

View full text Add to dashboard Cite

show abstract

“…However, when compared to the control group, the 150 mg/kg oxyresveratrol group showed changes in several biochemical and hematological parameters, as well as changes in the pathophysiology of cardiac, hepatic, and renal tissues. In conclusion, no observed adverse or harmful effect of ORV at 100 mg/kg in rats (89). On human, there are no toxicity studies reported on ORV long term use until now.…”

Section: Toxicity Of Oxyresveratrolmentioning

confidence: 71%

“…ORV at greater than 2000 mg/kg through oral gavage did not cause any mortality to the rats. Also, there was no change in behavior or locomotion during the study period (89). In addition, a sub-chronic toxicity test was performed in nine weeks with ORV at 50, 100 and 150 mg/kg by daily oral administration.…”

Section: Toxicity Of Oxyresveratrolmentioning

confidence: 99%

Effect of Oxyresveratrol on Inflammatory Skin Diseases||

Tran

View full text Add to dashboard Cite

Background:�Oxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is�Artocarpus lakoocha, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not been clearly demonstrated. Therefore, we investigated the anti-inflammatory effects of ORV on dermatitis model. Objectives:�To determine the effect and mechanism of ORV on anti-proliferation, anti-inflammation and anti-Staphyloccocus aureus�with keratinocytes and��to clarify the treating efficacy of ORV on skin inflammatory mouse model. Materials and methods:�The effect of ORV was examined on human immortalized and primary skin cells exposed to bacterial components including peptidoglycan (PGN) and lipopolysaccharide (LPS). PGN and LPS were used to induce inflammation on immortalized keratinocytes (HaCaT) and human epidermal keratinocytes (HEKa). We then performed MTT assay, Annexin V and PI assay, cell cycle analysis, real-time PCR, ELISA and Western blot in these�in vitro�models. On mouse model, 2,4-Dinitrochlorobenzene�(DNCB)�was used to induce dermatitis. H&E staining, immunohistochemistry (IHC) staining with CD3, CD4 and CD8 markers were used to evaluate the effects of ORV in�in vivo�model of skin inflammation using BALB/c mice. Disc diffusion method on�Staphyloccocus aureus�was used to test the anti-bacterial effect of ORV.� Results:�Treatment with ORV on HaCaT cells showed the anti-proliferation effect through inducing apoptosis by activating caspase-3. Pretreatment of HaCaT and HEKa cells with ORV inhibited pro-inflammatory cytokine production through inhibition of NF-?B pathway. In DNCB-induced dermatitis mouse model, ORV treatment reduced severity of skin lesion, and skin thickness and numbers of CD3, CD4 and CD8 T cells in the sensitized skin of mice. The combination of ORV and several antibiotics at the dosage using on keratinocytes showed the synergestic effect on�anti-Staphyloccocus aureus�through the increase of inhibiting zone.� Conclusion:�ORV treatment can ameliorate inflammation in the in vitro models of skin inflammation and in vivo models of dermatitis, suggesting a therapeutic potential of ORV for treatment of skin diseases particularly eczema. ORV can facilitate the anti-bacterial property of antibiotics on�Staphylococcus aureus.�

show abstract

Natural products as potential modulators of pro-inflammatory cytokines signalling in Alzheimer's disease

Yadav,

Kumarasamy

2024

Brain Behavior and Immunity Integrative

View full text Add to dashboard Cite

Biochemical and histopathological analysis after sub-chronic administration of oxyresveratrol in Wistar rats

Cited by 6 publications

References 48 publications

Sub-Chronic Toxicological Evaluation of the Sesquiterpene Lactone-Enriched Fraction of Tithonia diversifolia (Hemsley) A. Gray in Experimental Rats

Sub-Chronic Toxicological Evaluation of the Sesquiterpene Lactone-Enriched Fraction of Tithonia diversifolia (Hemsley) A. Gray in Experimental Rats

Effect of Oxyresveratrol on Inflammatory Skin Diseases||

Natural products as potential modulators of pro-inflammatory cytokines signalling in Alzheimer's disease

Contact Info

Product

Resources

About