Biochemical and Histomorphological Study of Streptozotocin-Induced Diabetes Mellitus in Rabbits

Mir, Sajad Hussain; Baqui, Abdul; Bhagat, Ruchi; Darzi, M.M.; Shah, Abdul Wahid

doi:10.3923/pjn.2008.359.364

Cited by 24 publications

(15 citation statements)

References 15 publications

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“…Save for the observed decline in serum albumin (2 mg/kg bw), lack of effect of treatment on visceral organ weights, serum lipid profile, liver function biomarkers, serum protein and bilirubin levels ( Tables 1,5 and 6) are consistent with a specific effect of quassin on reproductive system, without systemic toxicity at doses administered [27]. Parveenet et al [7] earlier reported that Q. Amara extract had no effect on hematological and serological parameters.…”

Section: Discussionsupporting

confidence: 50%

Implication of Hongres1 Protein in Quassin-Induced Male Reproductive Abnormality in Rats

Y¹

2014

Endocrinol Metab Synd

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Objective: Quassin, the bioactive constituent of Quassia amara plant, has been reported to decrease sperm count, sperm motility, normal sperm morphology and male fertility index. These sperm characteristics are however acquired in the epididyimis. Information on the effects of quassin on epididymal functions is scanty. The effects of quassin on epididymal functions were therefore investigated in Wistar albino rats.Methods: Quassin (0.1 and 2 mg/kg) and distilled water (0.5 ml) were administered by gavage (p. o) daily for 6 weeks to male rats (180-200 g, n=5), and thereafter sacrificed. Sperm motility, viability and count were examined microscopically. Serum from each rat was analyzed for Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone by enzyme immunoassay technique. Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST) and Alkaline Phosphatase (ALP) were measured spectrophotometrically. Epididymal HongrES1 protein expression was assessed using immune histochemical technique. Data were analyzed using Student's t-test at p=0.05. Results:Quassin significantly decreased sperm motility, % viability and count. Serum FSH, LH and testosterone were also decreased. The epididymal tubules were coiled and hyperplastic, while no visible lesion was observed on the testes. Serum ALT, AST, LDL, HDL, cholesterol, triglyceride, bilirubin and total protein levels were not affected by quassin. Total epididymal protein was however significantly reduced while total testicular protein increased. Epididymal HongrES1 protein expression was suppressed by treatment with quassin. Conclusion:Quassin decreased epididymal tissue total protein and HongrES1 protein expression in rats. These effects indicate that antifertility action of quassin is pronounced in the epididymis.

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Section: Discussionsupporting

confidence: 50%

Implication of Hongres1 Protein in Quassin-Induced Male Reproductive Abnormality in Rats

Y¹

2014

Endocrinol Metab Synd

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“…29,30 Administration of STZ to rats caused an abnormally high level of glucose, triglycerides and total cholesterol in the serum of diabetic rats compared to saline control as previously reported studies. 31 In the present investigation, the glucose level was abnormally increased (80.65%) in STZ treated rats in 8 th week, drug treatment for 14 days (7 th & 8 th weeks), resulted in decrease of glucose level (42.22%) and (46.42%) at dose of 250 and 500mg.kg -1 respectively in normal rats. However, drug treatment in diabetic rats for the same time period resulted in decreasing the glucose level (35.71%) and (45.94%) at the dose of 250 and 500mg.kg -1 respectively.…”

Section: Discussionsupporting

confidence: 46%

Effect of methanolic extracts of Tectona grandis linn leaves on diabetic neuropathy in streptozotocin‒induced diabetic rats

Kushwah¹

2018

MOJDDT

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“…Signicant reductions in the activities of these enzymes in EHN treated diabetic rats indicated the hepato protective role in preventing diabetic complications. Additionally, our data showed that total protein, albumin, globulin and a:g ratio concentration in the serum of STZ-NC induced diabetic rats was signicantly decreased (P<0.01) compared to normal control rats which is a reection of cytotoxicity as [37][38] reported in the independent studies . Interestingly, treatments with different doses of EHN and glibenclamide signicantly reversed the protein concentrations in diabetic rats ( Table 3).…”

Section: Protein Levelssupporting

confidence: 55%

Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Ethanolic Extract of <i>Helleborus niger</i> Linn Roots in Streptozotocin - Nicotinamide Induced Diabetic Rats

Kumar¹,

Lalitha²

2014

Journal of Pharmaceutical Research

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Helleborus niger L (HN) is widely used in the treatment of diabetes mellitus in the Indian traditional system of medicine. Therefore, the present study was done to evaluate the antihyperglycemic, antihyperlipidemic and antioxidant activities of ethanol extract of HN root (EHN) in streptozotocin (STZ)-nicotinamide (NC) induced diabetic rats. in vitro α-amylase inhibition assay, normoglycemic study and oral glucose tolerance test were carried out in different root extracts of HN. Antihyperglycemic effect was assessed in EHN using diabetic rats, which was identied as the most effective extract by initial screening. EHN (50, 100 and 200 mg/kg) and glibenclamide (5 mg/kg) orally administered daily for 28 days and the animal was observed in next 14 days. Fasting blood glucose (FBG) and body weight was determined weekly basis up to 42 days. On the 42nd day, various biochemical parameters were estimated. The three doses of EHN shows signicantly decrease (p<0.01) in blood glucose levels. The effect was more pronounced in 200mg/kg (71.53%) than 100mg/kg (67.46%) and 50mg/kg (66.63%). In addition, decreased HbA C and improved Hb level were 1 evidenced clearly in diabetic rats. Simultaneously, improvements in serum lipid prole, serum liver prole in diabetic rats were also evidenced clearly. Moreover, body weight and protein levels were increased in diabetic rats. On the other hand antioxidant activity was restored in diabetic rats. Increased glycogen content, glucokinase and decreased glucose-6 phosphatase, fructose 1, 6-biphosphatase effects in liver tissues were observed. EHN preserved islet architecture and prevented hypertrophy of β-cells. The EHN is capable of managing hyperglycemia and complications of diabetes in STZ-NC induced diabetic rats. Hence this plant may be considered as one of the potential sources for the isolation of new oral antihyperglycemic agent(s).

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Biochemical and Histomorphological Study of Streptozotocin-Induced Diabetes Mellitus in Rabbits

Cited by 24 publications

References 15 publications

Implication of Hongres1 Protein in Quassin-Induced Male Reproductive Abnormality in Rats

Implication of Hongres1 Protein in Quassin-Induced Male Reproductive Abnormality in Rats

Effect of methanolic extracts of Tectona grandis linn leaves on diabetic neuropathy in streptozotocin‒induced diabetic rats

Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Ethanolic Extract of <i>Helleborus niger</i> Linn Roots in Streptozotocin - Nicotinamide Induced Diabetic Rats

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