Biochemical and histological investigation on the protective effect of poly-herbal extract in high salt diet-fed male Wistar rats

Olorunnisola, Olubukola Sinbad; Adegbola, Peter Ifeoluwa; Ajilore, Bamidele Stephen; Zabdiel, Abijo Ayodeji; Ajayi, Ayodeji Folorunsho; Fadahunsi, Olumide Samuel

doi:10.1016/j.phyplu.2021.100116

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…is result agrees with other studies showing that there is a relationship between hyperlipidaemia and pathological alteration of heart organs in rats [53]. Histopathological studies are the most precise evidence for the protective effect of drugs as protectants [54].…”

Section: Discussionsupporting

confidence: 92%

Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats

Obidah

Umaru

Adamu

2022

Biochemistry Research International

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The effect of iron oxide nanoparticles (FeONPs) synthesized using Spinacia oleracea leaf extract on Triton X-100-induced atherosclerosis in white Wistar rats was determined. FeONPs were characterized to determine their size, structure, composition, and shape. In vitro antioxidant activity of FeONPs against 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) was determined. Atherosclerosis was induced by intraperitoneal administration of 5% Triton X-100 (100 mg/kg body weight) for 14 days. Group 1 received standard rat chow and water. Group 2 received 100 mg/kg body weight of Triton X-100 and a standard diet. Group 3 received 100 mg/kg body weight of Triton X-100 followed by 20 mg/kg body weight of atorvastatin for 21 days. Groups 4, 5, and 6 received 100 mg/kg body weight Triton X-100 was followed by variable concentrations of 100, 300, and 500 µg/kg body weight FeONPs, respectively, for 21 days. Blood samples were analyzed for lipid, liver, antioxidant, and cardiovascular markers. Histopathology of the heart was also examined. Characterization revealed the amorphous nature, functional groups, and clustered topography of FeONPs. An upregulated antioxidant activity of FeONPs was observed in a dose-dependent manner. Administration of Triton X-100 showed elevated levels of lipid biomarkers except for high-density lipoprotein (HDL), which decreased in group 2 in comparison to group 1. Liver, antioxidant, and cardiovascular biomarkers all significantly increased. The structural alteration was observed in the heart tissue following histopathology examination. Administration of FeONPs significantly decreased all biomarkers and increased the level of HDL. Also, tissue architecture was restored. Our findings demonstrated that FeONPs were effective in ameliorating Triton X-100-induced atherosclerosis in rats.

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Section: Discussionsupporting

confidence: 92%

Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats

Obidah

Umaru

Adamu

2022

Biochemistry Research International

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Antihyperlipidemic effect of bread fortified with potato peel powder against Triton X-100-induced hyperlipidemia in male albino rats

Soltan,

Gazwi,

Ragab

et al. 2023

Journal of Functional Foods

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Chemical Composition and Antioxidant Activity of <i>Ganoderma lingzhi</i> and <i>Ganoderma lucidum</i> Fruiting Body Extracts

Harbatsevich,

Zenevich,

Batalova

et al. 2024

Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo pr

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INTRODUCTION. Ganoderma spp. have been used as a traditional oriental medicine and a bioactive dietary supplement. These fungi are a promising source of effective antioxidants. Currently, there is no regulatory framework to control the quality of this herbal drug and its bioactive components in the Russian Federation and the Republic of Belarus. Therefore, it is essential to study the chemical composition and pharmacological activity spectrum of G. lingzhi and G. lucidum extracts.AIM. The aim of this study was to determine the chemical composition and antioxidant activity of G. lingzhi and G. lucidum fruiting body extracts.MATERIALS AND METHODS. The study focused on pure cultures of G. lingzhi and G. lucidum obtained from the fungal species collection of the Forest Institute of the National Academy of Sciences of Belarus. Fungal biomass was grown using two substrates, including alder sawdust (1–3 mm fraction) and oak shavings (5–10 mm fraction). The fungal biomass was extracted using repeated maceration with 70% ethanol. The study tested the free radical-scavenging activity of the extracts in reactions with the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the radical cation derived from 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The chemical composition was analysed by high-performance liquid chromatography–mass spectrometry (HPLC–MS). The assays for phenols, steroids, and triterpenes used spectrophotometry.RESULTS. The extract of G. lucidum strain 334 cultivated on the alder substrate demonstrated the highest free radical-scavenging activity (IC50 =3.1±0.2 μg/mL (DPPH), IC50 =3.7±0.2 μg/mL (ABTS)), the highest phenolic content (326.2±16.5 μmol/g), and the highest triterpene content (2.00±0.11 mmol/g) of all the studied extracts. The antioxidant activity of the extracts of G. lingzhi and G. lucidum may be attributed to the content of ganoderic acid D, lucidenic acid D, naringenin, and other phenolic compounds.CONCLUSION. The high yield of extracts with a significant radical-scavenging activity makes artificially cultivated G. lingzhi and G. lucidum mushrooms a promising source of natural antioxidants.

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Biochemical and histological investigation on the protective effect of poly-herbal extract in high salt diet-fed male Wistar rats

Cited by 3 publications

References 22 publications

Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats

Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats

Antihyperlipidemic effect of bread fortified with potato peel powder against Triton X-100-induced hyperlipidemia in male albino rats

Chemical Composition and Antioxidant Activity of <i>Ganoderma lingzhi</i> and <i>Ganoderma lucidum</i> Fruiting Body Extracts

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