Biochemical and hematological effects of acute and sub-acute administration to ethyl acetate fraction from the stem bark Scutia buxifolia Reissek in mice

Silva, Andreia R.H. da; Moreira, Laís da Rosa; Brum, Evelyne da Silva; Freitas, Mayara Lutchemeyer de; Boligon, Alexandra Augusti; Athayde, Margareth Linde; Roman, Silvane Souza; Mazzanti, Cinthia M.; Brandão, Ricardo

doi:10.1016/j.jep.2014.03.063

Cited by 50 publications

(25 citation statements)

References 31 publications

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“…The methanolic extracts of both vegetables contained the highest concentrations of TPC. This was similar to the observation of Corpuz, Osi, and Santiago (2013) where aqueous extracts of S. siliquosum presented the lower amount of TPC in mg g −1 than alcoholic extracts. TFC of L. taraxacifolia for all extracts were also significantly (p < 0.05) higher (1.21-78.79 mg/100g) than C. ruben (0.85-60.61 mg/100g).The aqueous extracts of both however were significantly (P < 0.05) higher than the alcoholic extracts in this study.…”

Section: Discussionsupporting

confidence: 90%

“…The ability of L. taraxacifolia and C. rubens to effectively inhibit free radicals (OH, NO, DPPH) and their reducing properties in this study may be attributed to the presence of phenolic compounds with potent antiradical properties. These vegetables possess high antioxidant potential to boost the body antioxidants and protect humans against detrimental oxidative effects caused by free radicals (Shukla, Mehta, Bajpai, & Shukla, 2009;Corpuz et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Chromatographic analyses were carried out under gradient conditions using a C 18 column (4.6 mm × 250 mm) in reverse phase (HPLC-DAD with a Shimadzu Prominence Auto Sampler (SIL-20A) HPLC system, by Shimadzu, Kyoto, Japan), packed with 5 µm diameter particles, where the mobile phase was water containing 1% acetic acid (A) and methanol (B), and the composition gradient was: 5% of B until 10 min and changed to obtain 20%, 30%, 50%, 60%, 70%, 20% and 10% B at 20, 30, 40, 50, 60, 70 and 80 min, respectively, following the method described by da Silva et al (2014) with slight IJFS April 2017 Volume 6 pages 82-94 modifications. Each extract of L. taraxacifolia and C. rubens was analyzed at a concentration of 20 mg/mL.…”

Section: Quantification Of Alcohol Extracts By Hplc-dadmentioning

confidence: 99%

“…The limit of detection (LOD) and limit of quantification (LOQ) were calculated based on the standard deviation of the responses and the slope using three independent analytical curves. LOD and LOQ were calculated as 3.3 and 10 σ/S, respectively, where σ is the standard deviation of the response and S is the slope of the calibration curve (da Silva et al, 2014).…”

Section: Quantification Of Alcohol Extracts By Hplc-dadmentioning

confidence: 99%

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In vitro investigation of antioxidant activities of Launea taraxacifolia and Crassocephalum rubens

Borokini

Lajide

2017

IJFS

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Launea taraxacifolia and Crassocephalum rubens are among many wild, underutilized and under cultivated vegetables in Nigeria that are at risk of extinction. Total flavonoid contents (TFC), total phenolic contents (TPC), and antioxidant activities of different concentrations (1-5 mg ml −1 ) were evaluated; using in vitro assays to assess the scavenging properties of 2, 2-diphenyl-1-picryl hydrazyl (DPPHRSP), nitric oxide (NORSP) and hydroxyl (OHRSP). Phenolic profiles of the alcoholic extracts were characterized using high-performance liquid chromatography techniques. The results revealed higher TFC (mg/100g RE) in aqueous (6.06±0.02-78.79±0.01) than alcohol extracts (with methanol 0.93±0.01-12.73±0.04, and with ethanol -0.85±0.01-7.70±0.03). In a similar trend, OHRSP (%) was higher in aqueous extracts (40.83±0.10-91.74±0.19) than alcoholic extracts (with methanol -11.67±0.3-30.83±0.06; and with ethanol -14.42±0.06-40.27±0.05). TPC (mg/100g GAE) which was higher in alcoholic extracts (with methanol -21.48±0.01-133.20±0.16 and with ethanol -9.45±0.01-59.73±0.02) than aqueous extracts (14.83±0.01-52.64±0.03) was in agreement with the trend observed for NORSP (28.24±0.05-151.76±0.08 for methanolic extracts, 21.99±0.13-49.93±0.04 for ethanolic extracts and 38.47±0.11-86.15±0.05 for aqueous extracts). DPPHRSP was also higher in alcoholic extracts (methanolic -22.81±0.01-48.41±0.05 and ethanolic-14.53±0.01-62.68±0.07) than aqueous extracts (13.66±0.13-42.86±0.03). TFC, TPC and antioxidant activities showed concentration dependent increase and strong positive correlation with TFC (r= 0.926 -0.997and r= 0.432 -1.000) and TPC (r= 0.825 -0.999 and r= 0.473 -0.994) for L. taraxacifolia and C. rubens respectively. Caffeic acid, chlorogenic acid, ellagic acid, quercetin and kaempferol were identified as major phenolic components in the extracts. The vegetables have high antioxidant potential for promoting good health; which could be attributed to the identified phytochemicals in them.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Quantification Of Alcohol Extracts By Hplc-dadmentioning

confidence: 99%

Section: Quantification Of Alcohol Extracts By Hplc-dadmentioning

confidence: 99%

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In vitro investigation of antioxidant activities of Launea taraxacifolia and Crassocephalum rubens

Borokini

Lajide

2017

IJFS

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“…In the present study, complementing tests on the acute toxicity of a single dose of GFC were made [26] by oral administration repeated doses of GFC. This study was carried out with the aim of evaluating the qualitative and quantitative effects for a long period of exposure, providing latency for the installation of toxic effects and the consequent appearance after the accumulation of the substance in the body during preclinical trials.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Study Employing Repeated Doses of Garcinielliptone FC, a Polyisoprenylated-Benzophenone Isolated from Seed of Platonia Insignis Mart

Silva¹,

Filho²,

Júnior³

et al. 2015

JPP

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Abstract:The major constituent from the hexane extract of the seeds of P. insignis is GFC (garcinielliptone FC). Doses of 25, 50 and 75 mg/kg of GFC were aseptically suspended in 0.05% Tween 80 dissolved in 0.9% saline (vehicle) and orally administered for 30, 90 and 120 consecutive days to adult Swiss mice. In this work, the repeated oral administration, in animals of both sexes, demonstrates that this compound is not able to induce mortality and/or behavioral changes in adult mice. In addition, body weight gain, feed intake and disposal of excreta were not altered by the administration of this compound with repeated doses. Furthermore, no differences in weight and macroscopic structure of the brain, liver, kidney, lung, heart and spleen between groups of male and female adult mice were observed after treatment. During the periods of treatment, GFC produced no significant changes on haematological and biochemical parameters in male and female mice treated with all doses used. The aim of this study was to investigate the toxicological potential of GFC through behavioral, hematological, biochemical and morphological parameters in animals in order to ensure the safe use of Platonia insignis in folk medicine.

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Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

Oliveira

Widmer

Nascimento

et al. 2022

Chemistry & Biodiversity

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The ethanolic extract from Croton blanchetianus leaves has been shown to have antinociceptive activity in mice. Here, we investigated the antinociceptive activity of an ethyl acetate fraction (EAF) from this extract in mice and the possible pathways involved in the analgesic effect. Adverse effects on behavior and motor coordination were also evaluated. The EAF was characterized by liquid chromatography coupled with mass spectrometry and evaluated (12.5, 25, and 50 mg/kg per os) in the acetic acid-induced abdominal writhing, formalin, hot plate, and tail immersion assays. Naloxone, atropine, glibenclamide, prazosin, or yohimbine was pre-administered to mice to investigate the involved pathways in the formalin test. The open-field, rotarod, and elevated plus-maze tests were used to assess behavior and locomotion. The main components of the EAF were quercetin-3-O-(2-rhamnosyl) rutinoside, hyperoside, quercetin rutinoside pentoside, and quercetin hexoside deoxyhexoside. EAF showed antinociceptive effects in all models and was effective against both neurogenic and inflammatory pain. The reversion of the effects in the formalin test by naloxone and atropine revealed that the EAF acted via the opioid and cholinergic systems. In the open-field test, the behavior of the animals treated with the EAF was like that of control, except at the highest dose, when hypnosis, eyelid ptosis, decreased walking, hygiene, and rearing behaviors were observed. No muscle relaxant effect was observed, but an anxiogenic effect was observed at all doses. This study provides new scientific evidence on the pharmacological properties of C. blanchetianus leaves and their potential for the development of phytomedicines with analgesic properties.

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Biochemical and hematological effects of acute and sub-acute administration to ethyl acetate fraction from the stem bark Scutia buxifolia Reissek in mice

Cited by 50 publications

References 31 publications

In vitro investigation of antioxidant activities of Launea taraxacifolia and Crassocephalum rubens

In vitro investigation of antioxidant activities of Launea taraxacifolia and Crassocephalum rubens

Toxicological Study Employing Repeated Doses of Garcinielliptone FC, a Polyisoprenylated-Benzophenone Isolated from Seed of Platonia Insignis Mart

Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

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