BIOCHEMICAL AND HEMATOLOGICAL ALTERATIONS IN MICE INOCULATED WITH OUTER MEMBRANE PROTEIN, LIPOPOLYSACCHARIDES AND WHOLE CELLS OF &lt;I&gt;PASTEURELLA MULTOCIDA&lt;/I&gt; TYPE B: 2

Abdullah, Faez Firdaus Jesse; Adamu, Lawan; Osman, Abdinasir Yusuf; Haron, Abdul Wahid; Saharee, Abdul Aziz; Abdullah, Rasedee; Saad, Mohd Zamri; Zakaria, Zunita

doi:10.3844/ajavsp.2013.152.158

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Oral inoculation of Pasteurella multocida type B: 2 and its LPS into Balb c mice in the present study was able to produce histopathological changes in the vital organs. These findings were similar to those reported in the experimental infection of mice and buffalo calves via oral exposure [15,[26][27][28][29]. However, the use of graded doses of Pasteurella multocida type B: 2 and its LPS as used in this study was not previously reported in mice following oral inoculation.…”

Section: Discussionsupporting

confidence: 92%

Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in Mice

Ali¹,

Adamu²,

Abdullah³

et al. 2014

J Veterinar Sci Technol

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Pasteurella multocida type B: 2 is responsible for major animal diseases of economic importance in both developed and developing countries. Haemorrhagic Septicaemia (HS) could inflict devastating effects on blood tissues and organs in the host animal. Therefore, the current study aims to investigate the haematological and clinico-pathological responses in mice following oral inoculation of graded doses of Pasteurella multocida type B: 2 and its lipopolysaccharide. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n=10) and these were inoculated with 0.4 ml of PBS pH 7.4 orally. The treatment groups (B; n=25 and C; n=25) were inoculated with P. multocida type B: 2 and its lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B10 1 , B10 3 , B10 5 , B10 7 and B10 9 for Pasteurella multocida and C10 1 , C10 3 , C10 5 , C10 7 and C10 9 for LPS respectively. The mice were observed for 120 hours post-inoculation. The clinical signs (Ruffled fur, Ocular discharges, Level of alertness and Laboured breathing) were significantly different (p<0.001) in mice inoculated orally with variable doses of Pasteurella multocida type B: 2 and its LPS. RBC, PCV, haemoglobin concentrations, PT, APTT, Thrombocyte, WBC, Lymphocytes, monocytes, plasma proteins, band and segmented neutrophils were significantly different (p<0.0001) in mice inoculated with graded doses of Pasteurella multocida type B: 2 and its LPS. Inflammatory cells, degeneration, necrosis and congestion were significantly different (p<0.0001) in mice inoculated with graded doses of both Pasteurella multocida type B: 2 and its LPS. In conclusion, 10 9 cfu of Pasteurella multocida type B: 2 and its lipopolysaccharide have devastating effects on organs and blood tissues.

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Section: Discussionsupporting

confidence: 92%

Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in Mice

Ali¹,

Adamu²,

Abdullah³

et al. 2014

J Veterinar Sci Technol

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“…However, the humoral immune response to LPS varies and is dependent upon the host animal and on variations within the LPS structure (Harper et al, 2011;Hodgson et al, 2013). Although most of experimental infection using LPS of P. multocida B:2 focused on clinical and pathological changes in the respiratory and gastrointestinal tracts (Horadagoda et al, 2002;Abdullah et al, 2013a), less attention has been paid on the nervous system. However, recent study by Jesse et al (2014) demonstrated the ability of LPS of P. multocida B:2 to induce histopathological changes in pituitary gland in male and female mice and finally change the hormonal levels.…”

Section: Virulence Factorsmentioning

confidence: 99%

“…The role of cytokines in the pathogenesis of P. multocida infection has been studied using murine and buffalo models, and a surge in pro-inflammatory cytokines that results in inflammatory reaction in the tissues were attributed in pathogenesis of the disease. In addition, the release of cytokines activates the acute phase response (APR) (Horadagoda et al, 2001;Praveena et al, 2010;Abdullah et al, 2013a). The most notable cytokines are TNFα, IL-1β, IL-6, IL-8 and IL-12 (Eckersall, 2006;Cray et al, 2009).…”

Section: Cytokines and Acute Phase Proteins Effect Due To P Multocida...mentioning

confidence: 99%

“…The most notable cytokines are TNFα, IL-1β, IL-6, IL-8 and IL-12 (Eckersall, 2006;Cray et al, 2009). However, IL1β, IL-6 and TNFα have been shown able to reproduce toxic effects induced by LPS of P. multocida (Horadagoda et al, 2001;Harper et al, 2011;Abdullah et al, 2013a;Abubakar et al, 2013;Ali et al, 2014). Moreover, administration of LPS has been reported to induce neuroinflammation and increases pro-inflammatory cytokines, such as IL-1β, IL-6 and TNF-α (Song and Wang, 2011).…”

Section: Cytokines and Acute Phase Proteins Effect Due To P Multocida...mentioning

confidence: 99%

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Involvement of nervous system in cattle and buffaloes due to Pasteurella multocida B:2 infection: A review of clinicopathological and pathophysiological changes

Marza¹,

Abdullah²,

Ahmed³

et al. 2015

J Adv Vet Anim Res

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Hemorrhagic septicemia (HS) is an acute septicemic disease principally affecting cattle and buffaloes caused by specific serotypes B:2 and E:2 of Pasteurella multocida in Asia and Africa, respectively. Despite continuing researches on pathogenesis of P. multocida for several decades, the mechanisms by which these bacteria develop the diseases are poorly understood. Although the involvement of the nervous system in the disease progress of HS is rare under natural conditions, few reports indicated the involvement of the nervous system in outbreaks of HS in cattle and buffaloes. Additionally, recent pathogenesis studies in both mouse and buffalo experimental models reported the involvement of nervous system due to P. multocida B:2, with bacteriological and histopathological evidences. In this review, we summarized and discussed the updates on the involvement of the nervous system in pathogenesis of HS focusing on clinical signs, pathological and pathophysiological changes.

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Clinico-pathology, hematology and biochemistry responses in buffaloes towards Pasteurella multocida type B: 2 immunogen lypopolysaccharide via oral and intravenous routes of infection

Chung

Abdullah

Ibrahim

et al. 2016

Microbial Pathogenesis

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Haemorrhagic septicaemia is a disease caused by Pasteurella multocida serotype B: 2 and E: 2. The organism causes acute, highly fatal septicaemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Lipopolysaccharide can be found on the outer cell wall of the organism. Lipopolysaccharide is released during multiplication which leads to inflammatory reaction. It represents the endotoxin of P. multocida type B: 2 and responsible for toxicity in haemorrhagic septicaemia which plays an important role in the pathogenesis of the disease. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, gross post mortem lesions and histopathology changes caused by P. multocida type B:2 immunogen lipopolysaccharide infections initiated through intravenous and oral routes of infection. 9 buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline (PBS); Group 2 and 3 were inoculated with 10 ml of lipopolysaccharide broth intravenously and orally respectively. For the clinical signs, there were significant differences (p < 0.05) in temperature between the control, intravenous and oral group. In hematology and biochemistry findings, there were significant differences (p < 0.05) in erythrocytes, haemoglobin, PCV, MCV, lymphocytes, monocytes, eosinophils, GGT and albumin between the control, intravenous and oral group. However, there were no significant differences (p > 0.05) in the MCHC, leukocytes, band neutrophils, basophils, thrombocytes, plasma protein, icterus index, total protein, globulin and A:G ratio between intravenous and oral group. For Group 2 buffaloes, there were gross lesions in the lung, trachea, heart, liver, spleen, and kidney. In contrast, lesions were only observed in the lung, trachea and liver of Group 3 buffaloes. There were significant differences (p < 0.05) in hemorrhage and congestion; necrosis and degeneration; and inflammatory cells infiltration between experimental groups and control group. However, there were no significant differences (p > 0.05) in edema lesion between groups. In conclusion, this study is a proof that oral route infection of P. multocida type B:2 immunogen lipopolysaccharide can be used to stimulate host cell responses where oral vaccine through feed could be developed in the near future.

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BIOCHEMICAL AND HEMATOLOGICAL ALTERATIONS IN MICE INOCULATED WITH OUTER MEMBRANE PROTEIN, LIPOPOLYSACCHARIDES AND WHOLE CELLS OF <I>PASTEURELLA MULTOCIDA</I> TYPE B: 2

Cited by 4 publications

References 13 publications

Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in Mice

Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in Mice

Involvement of nervous system in cattle and buffaloes due to Pasteurella multocida B:2 infection: A review of clinicopathological and pathophysiological changes

Clinico-pathology, hematology and biochemistry responses in buffaloes towards Pasteurella multocida type B: 2 immunogen lypopolysaccharide via oral and intravenous routes of infection

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