Biochemical and genetic studies on rabbit hemoglobin. I. Electrophoretic polymorphism of the? chain

Abstract

A genetic poIymorphism of/3-chain rabbit (Oryctolagus cuniculus) hemoglobin is demonstrated by means of acid starch gel electrophoresis. The biochemical evidence presented suggests that a previously reported substitution of a neutral amino acid for a histidine is responsible for the detected genetic variation. Segregation analysis was performed in a sample

Genetic polymorphism of a haemoglobin chain and adenosine deaminase in European shads: evidence for the existence of two distinct genetic entities with natural hybridization

“…Muscle homogenates were prepared as previously described (Alexandrino et al, 1993). Haemolysates were obtained by dilution of red cells in distilled water (1 : 8) followed by ultrasonic disintegration in an equal volume of toluene and centrifugation at 13 000 rpm for 5 min at 0 C. For ADA separation, haemolysates and muscle homogenates were diluted 5 : 1 with a 120 m solution of dithiothreitol (DTT) and incubated for 1 h at 40 C. Detection of haemoglobin variability was carried out after separation of the globin fractions according to Chernoff & Pettit (1964) with the modifications described by Ferrand (1989). The separated globin fractions were dissolved in 100 l of distilled water and then diluted 1 : 3 with a 8  urea and 50 m dithiothreitol solution for 1 h at 40 C.…”

Relaxed functional constraints on triplicate α-globin gene in the bank vole suggest a different evolutionary history from other rodents

“…Relaxed evolutionary constraints on a rodent a-globin gene S Marková et al example, Ferrand, 1989) and separated them by cellulose acetate membrane electrophoresis with a tris-borate-EDTA buffer (pH 8.9) in 8 M urea (Duffy et al, 1976). The electrophoresis was carried out at 450 V for 105 min at 4 1C.…”

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“…4 7 9 v 1 | CC-B Y 4 . 0 Op e n A c c e s s | r e c e i v e d : 2 7 A u g 2 0 1 4 , p u b l i s h e d : 2 7 A u g PrePrints (Ferrand, 1989;Biju-Duval et al, 1991;Geraldes et al, 2008;Carneiro et al, 2012), temporal (Hardy et al, 1994;Monnerot et al, 1994) and geographical perspectives (Webb et al, 1995;Fuller, Wilson & Mather, 1997;Surridge et al, 1999b;Queney et al, 2000;Queney et al, 2001;Branco et al, 2002).…”