Biochemical and Genetic Mechanisms Used by Plant Growth Promoting Bacteria

Glick, Bernard R.; Patten, Cheryl L.; Holguín, Gina; Penrose, Donna M.

doi:10.1142/p130

Cited by 510 publications

(315 citation statements)

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“…The bacteria accomplish this by breaking down the immediate precursor of ethylene, 1-aminocyclopropane 1-carboxylate (ACC), by ACC deaminase [17][18][19]22].…”

Section: Introductionmentioning

confidence: 99%

“…that also reduces the need for chemical fertilizers [1]. Although the presence of ACC deaminase has been detected in a number of soil bacteria and fungi [19], to date it has not been found in plants and is not present in Azospirillum [25]. We previously reported expression of the ACC deaminase gene (acdS) from Enterobacter cloacae UW4 in A. brasilense Cd by transferring into the bacterium a construct that involved acdS under the control of the Escherichia coli lac promoter.…”

Section: Introductionmentioning

confidence: 99%

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Transformation of Azospirillum brasilense Cd with an ACC Deaminase Gene from Enterobacter cloacae UW4 Fused to the Tet r Gene Promoter Improves Its Fitness and Plant Growth Promoting Ability

Holguín¹,

Glick

2003

Microbial Ecology

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A B S T R A C TIt has been reported that PGPB, containing ACC deaminase, can cleave the plant ethylene precursor ACC and thereby lower ethylene concentration in a developing or stressed plant, protecting it against the deleterious effects of stress ethylene and facilitating the formation of longer roots. In a previous work we have demonstrated expression of the ACC deaminase gene (acdS) from Enterobacter cloacae UW4 under the control of the lac promoter in Azospirillum brasilense Cd. With the inference that a construct including the ACC deaminase gene under the control of a constitutive promoter weaker than the lac promoter might impose less metabolic load on Azospirillum and improve its fitness, it was decided to clone acdS under the control of a tetracycline resistance gene promoter. The ACC deaminase structural gene was fused to the Tet r gene promoter by overlap extension using PCR, cloned in pRK415, and transferred into A.brasilense Cd. The resulting transformants showed lower ACC deaminase activity than those with the lac promoter controlled acdS gene. However, acdS under the control of the Tet r gene promoter imposed lesser metabolic load on Azospirillum brasilense Cd. The result was significantly increased IAA synthesis and greater bacterial growth rate, as well as increased ability to survive on the surface of tomato leaves and to promote the growth of tomato seedlings.

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“…The bacteria accomplish this by breaking down the immediate precursor of ethylene, 1-aminocyclopropane 1-carboxylate (ACC), by ACC deaminase [17][18][19]22].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transformation of Azospirillum brasilense Cd with an ACC Deaminase Gene from Enterobacter cloacae UW4 Fused to the Tet r Gene Promoter Improves Its Fitness and Plant Growth Promoting Ability

Holguín¹,

Glick

2003

Microbial Ecology

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“…Araujo (2008) has verified that presence of B. subtilis in lots of maize, cotton, and soybean seeds has positively influenced the metabolism and physiology of plants, what is reflected on increase of emergence, growth, and nutrition of plants. According to Glick et al (1999), bacteria may promote vegetal growth by direct stimulus so much by providing plant hormones, such as auxins and cytokinins, as for lowering the levels of ethylene, by means of the ACC deaminase enzyme. For Bewley e Black (1978) the emergence of seedling can be stimulated by acidification of cell wall during germination process, in such case being essential the action of auxin.…”

Section: Resultsmentioning

confidence: 99%

“…These so called plant growth-promoting rhizobacteria (PGPR) can colonize different plant organs and exert beneficial effects on them; and are also able of promoting increase on seed germination rate under glasshouse conditions, as well as in the field (Kloepper and Schroth, 1978;Amorim and Melo, 2002;Dey et al, 2004;Suslow and Schroth, 1982). Such benefits can occur through production of hormones and/or enzymes by these bacteria (Glick et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Physiological potential of rice seeds treated with rhizobacteria or the insecticide thiamethoxam

Soares

Tillmann

Moura

et al. 2012

Rev. bras. sementes

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ABSTRACT-Development of new technologies, aiming at increasing productivity in different crops, involves constant research on the effectiveness and application of these techniques in seed treatment. In this study, it was aimed at evaluating physiological potential of rice seeds treated with plant growth-promoting rhizobacteria (PGPR) (strains DFs185, DFs223, DFs306, DFs416), or with two dosages of the insecticide thiamethoxam. The variables assessed were: germination (G); first count of germination (FCG); cold test (CT); length of seedlings aerial parts (LAP), root system (LRS), and total length (TL); emergence speed index (ESI); emergence (E), at 14 days; and dry phytomass (DP). Treatments have had a positive effect on percentage of G, FCG and E. The strain DFs185 has promoted increase in percentage emergence, for five of the six lots assessed. The variables: LAP; LRS; TL; ESI; and DP have undergone low or none influence of treatments; and there has been no toxic effect of rhizobacteria or insecticide thiamethoxam. In the cold test, a negative effect of treatments has been detected. Seed treatment with rhizobacteria, as well as with thiamethoxam, improve quality of low quality rice seeds. The strain DFs185 is promising for treating rice seeds, once it stimulates seed germination and emergence.

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“…A substantial increase in plant biomass due to P. fluorescens or T. harzianum inoculation, depicted microbes mediated improvement in plant nutrition (Clark and Zeto 2000). Furthermore, P. fluorescens, a known plant growth-promoting bacterium, has also ensured bioavailability of phosphorus for plant uptake, fixing nitrogen for plant use, sequestering essential trace elements like iron by siderophores and producing plant hormones like auxins, cytokinins and gibberellins, beside cleaving the plant ethylene precursor ACC and thus lowering the ethylene level in growing and/or stressed plants due to presence of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (Glick et al 1999). Naiman et al (2009) have also reported that inoculants of Azospirillum brasilense Az1 and Az2 and P. fluorescens Pf increased average aerial biomass by 12%, root biomass by 40% and grain yield of wheat by 16%.…”

Section: Discussionmentioning

confidence: 99%

Phytoextraction of soil cadmium and zinc by microbes-inoculated Indian mustard (Brassica juncea)

Chauhan

Rai

2009

Journal of Plant Interactions

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A pot experiment was carried out to evaluate the effect of Pseudomonas fluorescens and Trichoderma harzianum inoculation on the uptake of zinc (Zn) and cadmium (Cd) by Indian mustard (Brassica juncea) from the soil having three different concentrations of Zn (300, 600, 900 mg/kg) and Cd (5, 10, 15 mg/kg) separately. Microbial inoculation resulted in significantly better plant growth, available metal content and their uptake than control (without microbes). Available Zn was enhanced, ca.1.6-and 1.4-fold and Cd ca. 2.5-and 1.8-fold, by P. fluorescens and T. harzianum respectively. P. fluorescens resulted in an increase in Zn uptake by 113.9, 51.9 and 58.4% and T. harzianum by 42.6, 32.1 and 33.9% over control from soils having 300, 600 and 900 mg Zn, respectively, while of the corresponding results for Cd were 110.2, 48.9 and 58.1% with P. fluorescens and 42.6, 30.9 and 33.4% with T. harzianum from soil having 5, 10 and 15 mg Cd, respectively, after 90 days of treatment. In general the rate of metal uptake was higher during the initial 30 days and declined later.

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Biochemical and Genetic Mechanisms Used by Plant Growth Promoting Bacteria

Cited by 510 publications

References 0 publications

Transformation of Azospirillum brasilense Cd with an ACC Deaminase Gene from Enterobacter cloacae UW4 Fused to the Tet r Gene Promoter Improves Its Fitness and Plant Growth Promoting Ability

Transformation of Azospirillum brasilense Cd with an ACC Deaminase Gene from Enterobacter cloacae UW4 Fused to the Tet r Gene Promoter Improves Its Fitness and Plant Growth Promoting Ability

Physiological potential of rice seeds treated with rhizobacteria or the insecticide thiamethoxam

Phytoextraction of soil cadmium and zinc by microbes-inoculated Indian mustard (Brassica juncea)

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