Biochemical and genetic changes revealing the enhanced lipid accumulation in Desmodesmus sp. mutated by atmospheric and room temperature plasma

Li, Pengfei; Sun, Xin; Sun, Zhe; Huang, Feng; Wang, Wei; Liu, Xingshe; Liu, Yongjun; Deng, Linyu; Cheng, Zhiwen

doi:10.1016/j.renene.2021.03.048

Cited by 17 publications

(7 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After optimized fermentation, the enzyme activity of the mutant strain increased by 1.47 times. This also shows that ARTP mutagenesis can improve the function of organisms, which proves the reliability of the mutagenesis technology 86,101,119 …”

Section: Application Of Artp In the Field Of Mutagenesismentioning

confidence: 56%

“…The researchers have demonstrated that ARTP mutagenesis ensures stable inheritance of the target characteristics of the mutant strains, with excellent genetic stability over several generations. 22,23,85,86,118,140 For example, Akwagiobe et al, 153 obtained a mutant strain with high L-asparaginase production and stable inheritance for 15…”

Section: Artp Mutagenesis Of Edible Fungimentioning

confidence: 99%

“…These processes are easily repaired by eukaryotic organisms but not by bacteria. In addition, lipid peroxidation and structural changes in cell membrane surface binding proteins or enzymes will activate different intracellular signaling pathways 23,85–87 …”

Section: Artpmentioning

confidence: 99%

“…Real‐time quantitative PCR analysis showed that the mutant strain had higher lipid gene expression, further verifying that its lipid content was increased by ARTP mutagenesis. Li et al, 86 found that mutagenesis of Desmodesmus sp . by ARTP affected genes in the genetic information process (33.51%) and metabolic pathway (28.74%) of the KEGG pathway.…”

Section: Application Of Artp In the Field Of Mutagenesismentioning

confidence: 99%

“…It is necessary to ensure that the mutants with excellent characteristics will not undergo genotypic and phenotypic changes during subsequent passaging culture. The researchers have demonstrated that ARTP mutagenesis ensures stable inheritance of the target characteristics of the mutant strains, with excellent genetic stability over several generations 22,23,85,86,118,140 …”

Section: Genetic Stability Of Mutantsmentioning

confidence: 99%

See 4 more Smart Citations

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Li,

Shen,

Ding

et al. 2024

Cell Biochemistry & Function

View full text Add to dashboard Cite

At present, atmospheric and room‐temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment‐friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional mutagenesis strategies, ARTP is used mainly to change the structure of microbial DNA, enzymes, and proteins through a series of physical, chemical, and electromagnetic effects with the organisms, leading to nucleotide breakage, conversion or inversion, causing various DNA damages, so as to screen out the microbial mutants with better biological characteristics. As a result, in recent years, ARTP mutagenesis and the combination of ARTP with traditional mutagenesis have been widely used in microbiology, showing great potential for application. In this review, the recent progress of ARTP mutagenesis in different application fields and bottlenecks of this technology are systematically summarized, with a view to providing a theoretical basis and technical support for better application. Finally, the outlook of ARTP mutagenesis is presented, and we identify the challenges in the field of microbial mutagenesis by ARTP.

show abstract

Section: Application Of Artp In the Field Of Mutagenesismentioning

confidence: 56%