Biochemical and Clinicopathological Changes Induced by Bungarus coeruleus Venom in a Rat Model

Kiran, Karthika; More, Sunil S.; Gadag, J. R.

doi:10.1515/jbcpp.2004.15.3-4.277

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…A previous study in India using B. caeruleus venom, has demonstrated haemorrhage and necrosis of proximal tubules of the kidney and petechial haemorrhages in the myocardium (Kiran et al, 2004) in addition to findings similar to the present study.…”

Section: Bungarussupporting

confidence: 92%

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Histopathological Changes in Brain, Kidney and Liver of Mice following Intramuscular Administration of Krait Venom

Nanayakkara

Ranasinghe

2009

Ceylon J. Sci. (Biol. Sci.)

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Histopathological changes following krait (Bungarus) bites are not widely documented compared to other species of snakes. In this study, the histopathological changes in liver, kidney and brain tissues following intramuscular administration of venom of Bungarus ceylonicus and Bungarus caeruleus were studied. Serial dilutions of venom of the two species of snakes were injected to seven groups of mice after determination of protein concentrations. There were no macroscopically identifiable changes in any of the organs. Tissues were obtained for histological studies at 1 h, 3 h, 6 h, 12 h and 24 h time intervals following injection of venom. Histopathological changes namely congestion, inflammation and necrosis were observed microscopically in the tissues of liver, brain and kidney. These changes were proportionate to the dose of venom and were maximum in the liver at the dose of 1.0 µg/mouse for B. caeruleus venom and at 2.0 µg/mouse for B. ceylonicus venom. Histopathological changes observed due to B. caeruleus and B. ceylonicus venom appeared within 1 h in all tissues. Congestion was moderate in the liver and kidney but was less marked in brain. Inflammatory infiltration was seen in the perivascular regions in liver and kidney and was mainly lymphocytic. Venom of B. ceylonicus produced histopathological changes in the brain tissue, but not B. caeruleus venom. Histopathological changes were intense with B. ceylonicus venom than with B. caeruleus venom. Necrosis was seen in the liver and brain only following administration of B. ceylonicus venom. No histopathological changes were observed in skeletal and cardiac muscle with either venom.

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Section: Bungarussupporting

confidence: 92%

“…Degeneration, necrosis and regeneration of tubular epithelial cells are seen in renal tubules. Interstitial oedema, cellular infiltration, arteritis, thrombophlebitis, congestion, infarction and cortical necrosis have also been observed (Sitprija, 2006;Kiran et al, 2004).…”

Section: Introductionmentioning

confidence: 98%

Histopathological Changes in Brain, Kidney and Liver of Mice following Intramuscular Administration of Krait Venom

Nanayakkara

Ranasinghe

2009

Ceylon J. Sci. (Biol. Sci.)

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“…Cytoplasmic vacuolization, infiltration and congestion of blood vessels were major changes in cardiac tissue. The findings of current study are almost consistent with previous observations ( Al-Mamun et al, 2015a ; Kiran et al, 2004 ; Nanayakkara et al, 2009 ). However, in this study, clear and broad range images are presented for histopathological tissue sections of B. caeruleus envenomed mice.…”

Section: Discussionsupporting

confidence: 94%

Evaluation of the properties of Bungarus caeruleus venom and checking the efficacy of antivenom used in Bangladesh for its bite treatment

et al. 2023

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“…8–10 The morphological changes in the kidney in fatal snakebite cases are vividly elaborated in texts of forensic pathology. Studies have shown renal histological changes 13,14 induced by krait (neurotoxic) venom in animal model. We devised a scoring system on the basis of the renal pathology found at autopsy in fatal snakebite cases.…”

Section: Discussionmentioning

confidence: 99%

Forensic diagnosis and classification of snakebite (neurotoxic and haemotoxic) from renal changes at autopsy: morphological and histological determinants using a semi-quantitative method

Mukhopadhyay¹,

Ghosh

2010

Med Sci Law

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Mortality due to poisonous snakebite is an emerging public health concern. Of late, snakebite has drawn global attention. Snakebite is a well-known cause of renal pathology especially in the haemotoxic group. Deaths due to renal failure are very common in envenomation due to poisonous snakebite. In this investigation our aim was to devise autopsy-proven diagnostic criteria of snakebite from the renal changes to confirm and classify the type of snakebite in forensic casework. That would be of practical help to the forensic pathologist with access to limited resources. An arbitrary scoring system from histology was adopted for the present semi-quantitative analysis. The scoring system (with scores from 0 to 5) developed by the present study was effective, valid and was able to distinguish between snakebite and control. The score obtained at histology in cases of snakebite was significantly different from the control group (Mann-Whitney U = 71.5; P = 0.011). Using stepwise linear discriminant function (DF) analysis, the score (Mukhopadhyay's score [MS]) was seen to be the only predictor of the type of snakebite. This model (DF = 1.05 x MS-1.78) could correctly classify 93.3% of the cases into neurotoxic and haemotoxic variety of offending snake. This is an effective, cheap and easy semi-quantitative method of distinguishing neurotoxic and haematoxic snakes from the renal changes at autopsy. It would be useful for practical application in centres where sophisticated biochemical or immunological analyses are not available due to limited resources.

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Biochemical and Clinicopathological Changes Induced by Bungarus coeruleus Venom in a Rat Model

Cited by 6 publications

References 15 publications

Histopathological Changes in Brain, Kidney and Liver of Mice following Intramuscular Administration of Krait Venom

Histopathological Changes in Brain, Kidney and Liver of Mice following Intramuscular Administration of Krait Venom

Evaluation of the properties of Bungarus caeruleus venom and checking the efficacy of antivenom used in Bangladesh for its bite treatment

Forensic diagnosis and classification of snakebite (neurotoxic and haemotoxic) from renal changes at autopsy: morphological and histological determinants using a semi-quantitative method

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