Biochemical and biophysical properties of purified phospholipid vesicles containing bovine heart cytochrome c oxidase

Nguyen, Xuan-Thao; Pabarue, Hugh A; Geyer, R. Ryan; Shroyer, Lois A.; Estey, Lisa A.; Parilo, M.; Wilson, Kevin S.; Prochaska, Lawrence J.

doi:10.1016/s1046-5928(02)00503-x

Cited by 10 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of cytochrome oxidase decreases when the level of subunit III is reduced. However, the specific mechanism is unclear ( 15 – 17 ). In the present study, the stop codon of human ELCOX3 was shown to be T, as in Anopheles quadrimaculatus , Anopheles gambiae and Penaeus monodon (tiger prawns), and a polyA tail is required to be added to codon T as the final stop codon ( 18 – 20 ).…”

Section: Discussionmentioning

confidence: 99%

Cloning and expression analysis of the gastric carcinoma-related gene, ELCOX3

Gan¹,

Liu²,

Zhou³

et al. 2013

Oncology Letters

View full text Add to dashboard Cite

Gastric cancer is a pathological process of an accumulation of multigene and multistage mutations. A new gene segment, MDSCBC11, has been previously obtained using a gene chip and is negatively associated with gastric cancer. The present study aimed to clone the full cDNA sequence of the MDSCBC11 segment and to detect its expression in gastric carcinomas and normal gastric mucosa. Multiple-tissue northern blots revealed that the new MDSCBC11-represented gene was expressed as two transcripts that were 0.8 kb and 1.5 kb in size. The cDNA sequence of the smaller transcript was 822 bp, created by 5′ rapid amplification of cDNA ends (RACE) and 3′ RACE methods. A bioinformatics analysis indicated that the deduced amino acid sequence of MDSCBC11 had a 99% homology with the cytochrome c oxidase III (COX3) gene in the mitochondria. A total of 46 cases of gastric carcinomas, adjacent gastric mucosa and normal gastric mucosa were individually collected, and the mRNA expression of the ELCOX3 gene was detected by RT-PCR. ELCOX3 mRNA was expressed in all 46 cases of the normal gastric mucosa. The expression levels of ELCOX3 mRNA in the gastric carcinomas were lower compared with that of the adjacent and normal gastric mucosa (P<0.05), with the percent of downregulation at 23.91% (11/46 cases). The downregulation of ELCOX3 gene expression was associated with the development of human gastric carcinomas.

show abstract

Section: Discussionmentioning

confidence: 99%

Cloning and expression analysis of the gastric carcinoma-related gene, ELCOX3

Gan¹,

Liu²,

Zhou³

et al. 2013

Oncology Letters

View full text Add to dashboard Cite

show abstract

“… 9 Mitochondrial fractions were incubated with 0.38 mg, 2 mg, 2 mg and 5 mg Triton X-100/mg of protein in pig liver, yeast, cell lines and beef heart, respectively. 10 , 11 , 12 , 13 A suitable concentration of Triton X-100 applied to different samples needs to be tested.…”

Section: Limitationsmentioning

confidence: 99%

Protocol for mitochondrial isolation and sub-cellular localization assay for mitochondrial proteins

Zhou

Han

et al. 2023

STAR Protocols

View full text Add to dashboard Cite

Structure Determination of Functional Membrane Proteins using Small-Angle Neutron Scattering (SANS) with Small, Mixed-Lipid Liposomes: Native Beef Heart Mitochondrial Cytochrome c Oxidase Forms Dimers

et al. 2012

View full text Add to dashboard Cite

The low-resolution three-dimensional structure of purified native beef heart mitochondrial cytochrome c oxidase (COX) in asolectin unilamellar liposomes has been measured by small-angle neutron scattering under the conditions where the protein remains fully functional. From a neutron scattering perspective, the use of mixed-lipid liposomes provided for a more homogeneous matrix than can be achieved using a single lipid. As a result, the measurements were able to be performed under conditions where the liposome scattering was essentially eliminated (contrast-matched conditions). The protein structure in the membrane was modeled as a simple parallelepiped with side lengths of (59 × 70 × 120) Å with uncertainties, respectively, (11, 12, 20 Å). The molecular mass calculated for a typical protein with this volume is estimated to be (410 ± 124) kDa, which indicates the mass of a COX dimer. The longest dimension has some uncertainty due to intermolecular scattering contributing to the data. Nevertheless, that length was estimated using an average protein density and the known dimer molecular mass. Using the same cross sectional dimensions for the structure, the length is estimated to be 120 Å. However, the measured scattering curve of the dimer in the liposome differs significantly from that calculated from the X-ray structure of the dimer in a crystal of mixed micelles (PDB 3AG1). The calculated SANS scattering from the crystal structure was fit with a parallelepiped, measuring (59 × 101 × 129) Å with fitting uncertainties, respectively, (2, 3, 3 Å). Our results suggest that COX is a functional dimer when reconstituted into mixed-lipid liposomes.

show abstract

Biochemical and biophysical properties of purified phospholipid vesicles containing bovine heart cytochrome c oxidase

Cited by 10 publications

References 30 publications

Cloning and expression analysis of the gastric carcinoma-related gene, ELCOX3

Cloning and expression analysis of the gastric carcinoma-related gene, ELCOX3

Protocol for mitochondrial isolation and sub-cellular localization assay for mitochondrial proteins

Structure Determination of Functional Membrane Proteins using Small-Angle Neutron Scattering (SANS) with Small, Mixed-Lipid Liposomes: Native Beef Heart Mitochondrial Cytochrome c Oxidase Forms Dimers

Contact Info

Product

Resources

About