Biochemical and biophysical characterization of water-soluble pectin from Opuntia ficus-indica and its potential cytotoxic activity

Lefsih, Khalef; Giacomazza, Daniela; Passantino, Rosa; Costa, Maria Assunta; Bulone, Donatella; Mangione, Maria Rosalia; Guarrasi, Valeria; Mingoia, Francesco; Biagio, Pier Luigi San; Madani, Khodir

doi:10.1016/j.phytochem.2018.06.015

Cited by 16 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pectins are complex polysaccharides formed by homogalacturonan (α-1,4-linked d-galacturonic acid) polymer backbones with different decorations [61]. Rhamnogalacturonan I is a complex polysaccharide from plant cell walls and mucilage, which contains a linear backbone composed of diglycosyl repeating unit -4)-α- d -Galactopyranose-(1-2)-α- l -Rhamnopyranose [62,63], and has been identified in the prickly pear cactus O. ficus-indica [64]. Degradation of rhamnogalacturonan relies on multiple extracellular PL and GH enzymes [65].…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomic Analysis of the Bacterial Symbiont Dactylopiibacterium carminicum from the Carmine Cochineal Dactylopius coccus (Hemiptera: Coccoidea: Dactylopiidae)

Bustamante-Brito

León

Rosenblueth

et al. 2019

Life

View full text Add to dashboard Cite

The scale insect Dactylopius coccus produces high amounts of carminic acid, which has historically been used as a pigment by pre-Hispanic American cultures. Nowadays carmine is found in food, cosmetics, and textiles. Metagenomic approaches revealed that Dactylopius spp. cochineals contain two Wolbachia strains, a betaproteobacterium named Candidatus Dactylopiibacterium carminicum and Spiroplasma, in addition to different fungi. We describe here a transcriptomic analysis indicating that Dactylopiibacterium is metabolically active inside the insect host, and estimate that there are over twice as many Dactylopiibacterium cells in the hemolymph than in the gut, with even fewer in the ovary. Albeit scarce, the transcripts in the ovaries support the presence of Dactylopiibacterium in this tissue and a vertical mode of transmission. In the cochineal, Dactylopiibacterium may catabolize plant polysaccharides, and be active in carbon and nitrogen provisioning through its degradative activity and by fixing nitrogen. In most insects, nitrogen-fixing bacteria are found in the gut, but in this study they are shown to occur in the hemolymph, probably delivering essential amino acids and riboflavin to the host from nitrogen substrates derived from nitrogen fixation.

show abstract

Section: Discussionmentioning

confidence: 99%

Metatranscriptomic Analysis of the Bacterial Symbiont Dactylopiibacterium carminicum from the Carmine Cochineal Dactylopius coccus (Hemiptera: Coccoidea: Dactylopiidae)

Bustamante-Brito

León

Rosenblueth

et al. 2019

Life

View full text Add to dashboard Cite

show abstract

“…Pectin, a polysaccharide present in large amount in N. cochenillifera (Table 1) and in others such as cactus mucilage [30], is composed of acid D-galacturonic units joined in chains by α-(1→4) glycosidic bonds [31]. Pectin-free carboxylic groups with positive and negative partial charges may have adsorbed and aggregated the anionic and cationic polymers dissolved in the aqueous solution by the hydrogen bonds formation [32][33][34] or dipole and ion-dipole interactions [18], resulting in particle coagulation and, consequently, reduction in aqueous solution turbidity. The presence of numerous hydroxyl groups along the polysaccharide chain increases the intermolecular interaction points.…”

Section: Discussionmentioning

confidence: 99%

Nopalea cochenillifera Biomass as Bioadsorbent in Water Purification

Lima¹,

Silva²,

Cruz³

et al. 2021

Water

View full text Add to dashboard Cite

Contaminated water consumption is one of the greatest risks to human health, especially in underdeveloped and developing countries. Water is a universal right, but millions of people worldwide consume untreated surface water. The objective in this study is to evaluate water purification with Nopalea cochenillifera var. Miúda biomass. Fragments (1, 2, and 3 g) of N. cochenillifera were added to the aqueous solution containing red-yellow Chromic and Podzolic Luvisol simulating turbid water sources in Northeastern Brazil. The total, non-structural (i.e., reducing and non-reducing sugars, alcohol insoluble solids), and structural (i.e., pectin) carbohydrates, adsorption kinetics, turbidity, electrical conductivity, pH, zeta potential, and total coliforms presence were evaluated. Findings show that the Nopalea cochenillifera biomass adsorbed the suspended particles in the aqueous solution, making it more translucent due to the complex and heterogeneous adsorbents’ ion exchange matrix, but the biomass addition did not eliminate total coliforms from the aqueous solution. We concluded that the Nopalea cochenillifera biomass water treatment reduces suspended dissolved particles and turbidity, but it needs to be associated with other treatments to eliminate total coliforms and ensure water safety for human consumption.

show abstract

“…Cactus derivatives like pad juice and cladode mucilage are very effective components for water purification solely or in combination with wastewater treatment processes (Adjeroud et al, 2018(Adjeroud et al, , 2015Djerroud et al, 2018). Some other studies have been performed in Algeria on the extraction and characterization of mucilage from cladodes of O. ficus-indica (Adjeroud-Abdellatif et al, 2020;Adjeroud et al, 2018Adjeroud et al, , 2015Djerroud et al, 2018;Felkai-Haddache et al, 2016;Lefsih et al, 2018). Another study reported the phytochemical content and physical characteristics as well as the antioxidant activity of Algerian cactus pear fruits grown in northeastern region of the country (Mazari et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification of some Opuntia spp. from two Algerian regions and ultrasound-assisted extraction of their phenolic compounds

Mahdeb

Adjeroud‐Abdellatif

Mazari

et al. 2021

J PROF ASSOC CACTUS

Self Cite

View full text Add to dashboard Cite

Much attention has been paid to cacti for their distinctive characteristics. The nopal Opuntia ficus-indica and related species are found in arid and semi-arid regions around the world. O. ficus-indica shows considerable variability in response to environmental conditions through variation of areola and spine attributes, among other traits. Therefore, the present study sought to morphologically identify some cactus pear plants in two areas of Algeria (Bejaia and Souk-Ahras) in order to evaluate the cactus pears by optimizing the extraction conditions of the phenolic compounds of the cladode, to evaluate the antioxidant activity of these compounds, to measure the total flavonoid content, and to compare the yields among three Opuntia spp. (O. ficus-indica, O. megacantha and O. amyclae) from the sites within the study regions. These three Opuntia spp. were subjected to extraction of polyphenols using a conventional method, and an ecofriendly process using ultrasound-assisted extraction (UAE) technique. Once the extractions was completed, the total phenolic content and flavonoids of the extracts were characterized. In addition, the antioxidant capacity of the total phenolic compounds (TPC) was determined. The UAE was better than conventional extraction for the recovery of TPC. The use of a solvent mixture (50% water/ethanol, v/v) improved the TPC extraction efficiency. These results show that UAE was a useful tool for the extraction of phytochemicals from cactus pear cladodes. Other factors and conditions that influence the optimization of TPC extraction should also be studied, such as pH, ultrasound frequency as well as ecological factors that could possibly influence extraction efficiency.

show abstract

Biochemical and biophysical characterization of water-soluble pectin from Opuntia ficus-indica and its potential cytotoxic activity

Cited by 16 publications

References 39 publications

Metatranscriptomic Analysis of the Bacterial Symbiont Dactylopiibacterium carminicum from the Carmine Cochineal Dactylopius coccus (Hemiptera: Coccoidea: Dactylopiidae)

Metatranscriptomic Analysis of the Bacterial Symbiont Dactylopiibacterium carminicum from the Carmine Cochineal Dactylopius coccus (Hemiptera: Coccoidea: Dactylopiidae)

Nopalea cochenillifera Biomass as Bioadsorbent in Water Purification

Identification of some Opuntia spp. from two Algerian regions and ultrasound-assisted extraction of their phenolic compounds

Contact Info

Product

Resources

About