Biochemical and apoptotic changes in the nervous and ovotestis tissues of Biomphalaria alexandrina following infection with Schistosoma mansoni

Habib, Mohamed R.; Ghoname, Samah I.; Ali, Rasha E.M.; El-Karim, Rasha M. Gad; Youssef, Adel; Croll, Roger P.

doi:10.1016/j.exppara.2020.107887

Cited by 11 publications

(7 citation statements)

References 56 publications

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“…Fecundity at population level was also measured based on the parameter Mx (mean number of eggs / snail / week). Mx was computed according to Ragheb et al and Habib et al [ 63 , 64 ]. Egg hatching was also monitored every 2 days over 2 weeks.…”

Section: Methodsmentioning

confidence: 99%

Spatial variation of life-history traits in Bulinus truncatus, the intermediate host of schistosomes, in the context of field application of niclosamide in Côte d’Ivoire

Konan¹,

Tian-Bi²,

Diakité³

et al. 2022

BMC Zool

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Background Control of intermediate host snails using molluscicides for the control and/or elimination of schistosomiasis is a strategy in line with WHO recommendations. Niclosamide is the main chemical molluscicide recommended by WHO. However, except the immediate killing of the snail, the extent of the impact of the molluscicide application on the evolution of snail life-history traits, in relation to recolonization of treated sites is not well known. This study aimed to characterize the spatial variation of life-history traits of populations of the freshwater snail Bulinus truncatus, in relation to niclosamide spraying in the field. From 2016 to 2018, we conducted a trial, using niclosamide to control the intermediate host snails for interrupting the seasonal transmission of urinary schistosomiasis in northern and central Côte d’Ivoire. Five villages (sites) were considered, including three test and two control villages. In the test villages, the molluscicide was sprayed in habitats harboring B. truncatus snails three times a year (November, February–March and June). We sampled six B. truncatus populations: two populations from the control villages without any treatment; one collected before treatment and three sampled 2–3 months after treatment of the site with niclosamide. The snail populations were monitored for several life-history traits, including survival, growth, fecundity and hatchability, under laboratory conditions, over one generation (G1). We tested the population, region (North/Centre) and treatment status (treated/untreated) effects on the variation of the measured life-history traits and correlations between pairs of traits were estimated. Results On the whole, the traits varied among populations. The risk of death was lower in northern populations compared to central ones. The age at first reproduction was reached earlier with a smaller size of snails in northern populations. Values of first reproduction features (size and fecundity) were lower in treated snail populations. The overall growth of untreated populations was higher than that of treated ones. The late fecundity and egg hatching were higher in northern than in central snails. At first reproduction, age was negatively correlated with some fecundity parameters. However, growth was positively associated with fecundity. Conclusions Our study showed a spatial variation of life-history traits in B. truncatus snails. The mollusciciding seems to have led to the depression of some life-history traits in the snail populations. However, investigations should be carried out over several generations of snails to better clarify the impact of niclosamide on the evolution of the life-history traits.

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Section: Methodsmentioning

confidence: 99%

Spatial variation of life-history traits in Bulinus truncatus, the intermediate host of schistosomes, in the context of field application of niclosamide in Côte d’Ivoire

Konan¹,

Tian-Bi²,

Diakité³

et al. 2022

BMC Zool

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“…Furthermore, the relatively high expression of one of these proteins in the B. glabrata ovotestis may also be noteworthy because S. mansoni infections cause chemical castration in infected Biomphalaria , a phenomenon which has been well-characterised ( 66 ). Ovotestis are among the organs most affected by infection, as evidenced by the significantly decreased concentrations of sex hormones, such as estradiol and testosterone, post-exposure to S. mansoni miracidia ( 67 ). However, by 4 weeks PME, the concentrations of these hormones in the ovotestis were not significantly different compared to snails prior to infection.…”

Section: Discussionmentioning

confidence: 99%

Identification of Schistosoma mansoni miracidia attractant candidates in infected Biomphalaria glabrata using behaviour-guided comparative proteomics

et al. 2022

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Schistosomiasis, caused by infection with Schistosoma digenetic trematodes, is one of the deadliest neglected tropical diseases in the world. The Schistosoma lifecycle involves the miracidial infection of an intermediate freshwater snail host, such as Biomphalaria glabrata. Dispersing snail host-derived Schistosoma miracidia attractants has been considered a method of minimising intermediate host infections and, by extension, human schistosomiasis. The attractiveness of B. glabrata to miracidia is known to be reduced following infection; however, the relationship between duration of infection and attractiveness is unclear. Excretory-secretory proteins (ESPs) most abundant in attractive snail conditioned water (SCW) are key candidates to function as miracidia attractants. This study analysed SCW from B. glabrata that were naïve (uninfected) and at different time-points post-miracidia exposure (PME; 16h, 1-week, 2-weeks and 3-weeks PME) to identify candidate ESPs mediating Schistosoma mansoni miracidia behaviour change, including aggregation and chemoklinokinesis behaviour (random motion, including slowdown and increased turning rate and magnitude). Miracidia behaviour change was only observed post-addition of naïve and 3W-PME SCW, with other treatments inducing significantly weaker behaviour changes. Therefore, ESPs were considered attractant candidates if they were shared between naïve and 3W-PME SCW (or exclusive to the former), contained a predicted N-terminal signal peptide and displayed low identity (<50%) to known proteins outside of the Biomphalaria genus. Using these criteria, a total of 6 ESP attractant candidates were identified, including acetylcholine binding protein-like proteins and uncharacterised proteins. Tissue-specific RNA-seq analysis of the genes encoding these 6 ESPs indicated relatively high gene expression within various B. glabrata tissues, including the foot, mantle and kidney. Acetylcholine binding protein-like proteins were highly promising due to their high abundance in naïve and 3W-PME SCW, high specificity to B. glabrata and high expression in the ovotestis, from which attractants have been previously identified. In summary, this study used proteomics, guided by behavioural assays, to identify miracidia attractant candidates that should be further investigated as potential biocontrols to disrupt miracidia infection and minimise schistosomiasis.

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“…On the other hand, infected snails exposed to LED light had insufficient serotonin secretion, and so more uptake by miracidia had led to the depletion of serotonin levels in the surrounding tissue. One more explanation could be that LED light causes an increase in apoptosis to the sporocyst-surrounding tissue including nerve endings, which are responsible for the production and depletion of serotonin (Habib et al, 2020). Accordingly, this apoptosis might be less likely to increase serotonin levels.…”

Section: Discussionmentioning

confidence: 99%

Behavioral, Biochemical, and Histological Evaluation of Artificial Light on Infected Freshwater Snails Bimophalaria alexandrina by Schistosoma mansoni

al.¹

2022

Egypt. J. of Aquatic Biolo. and Fish.

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Schistosomiasis, commonly known as bilharzia, is an infection resulting from a parasitic worm that dwells in fresh water in subtropical and tropical areas. This parasitic worm (genus: schistosome) with its different life stages, including cercariae, miracidia, eggs, and adult worms, infects humans, some animals, as well as a specific intermediate host snail (McManus et al., 2018). Schistosoma spp. infects a minimum of 230 million

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Biochemical and apoptotic changes in the nervous and ovotestis tissues of Biomphalaria alexandrina following infection with Schistosoma mansoni

Cited by 11 publications

References 56 publications

Spatial variation of life-history traits in Bulinus truncatus, the intermediate host of schistosomes, in the context of field application of niclosamide in Côte d’Ivoire

Spatial variation of life-history traits in Bulinus truncatus, the intermediate host of schistosomes, in the context of field application of niclosamide in Côte d’Ivoire

Identification of Schistosoma mansoni miracidia attractant candidates in infected Biomphalaria glabrata using behaviour-guided comparative proteomics

Behavioral, Biochemical, and Histological Evaluation of Artificial Light on Infected Freshwater Snails Bimophalaria alexandrina by Schistosoma mansoni

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