Biochemical Anatomy of Human Bone: Comparative Study of Compact and Spongy Bone in Femur, Rib and Iliac Crest

Mbuyi-Muamba, J. M.; Dequeker, Jan

doi:10.1159/000146337

Cited by 18 publications

(5 citation statements)

References 7 publications

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“…Overall, these results are in agreement with published biochemical analyses, (46) which reported a depth-dependent increase in proteoglycan and collagen content for both immature and mature bovine cartilage. Similarly for subchondral bone and in agreement with previous studies, (57,58) a mineral-and collagen-rich matrix with a relatively low proteoglycan content was found. In this study, mature samples measure more collagen in the surface and deep zone, as well as a greater amount of proteoglycan in the deep zone.…”

Section: Discussionsupporting

confidence: 92%

FTIR-I Compositional Mapping of the Cartilage-to-Bone Interface as a Function of Tissue Region and Age

Khanarian

Boushell

Spalazzi

et al. 2014

Journal of Bone and Mineral Research

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Soft tissue-to-bone transitions, such as the osteochondral interface, are complex junctions that connect multiple tissue types and are critical for musculoskeletal function. The osteochondral interface enables pressurization of articular cartilage, facilitates load transfer between cartilage and bone, and serves as a barrier between these two distinct tissues. Presently, there is a lack of quantitative understanding of the matrix and mineral distribution across this multitissue transition. Moreover, age-related changes at the interface with the onset of skeletal maturity are also not well understood. Therefore, the objective of this study is to characterize the cartilage-to-bone transition as a function of age, using Fourier transform infrared spectroscopic imaging (FTIR-I) analysis to map region-dependent changes in collagen, proteoglycan, and mineral distribution, as well as collagen organization. Both tissue-dependent and age-related changes were observed, underscoring the role of postnatal physiological loading in matrix remodeling. It was observed that the relative collagen content increased continuously from cartilage to bone, whereas proteoglycan peaked within the deep zone of cartilage. With age, collagen content across the interface increased, accompanied by a higher degree of collagen alignment in both the surface and deep zone cartilage. Interestingly, regardless of age, mineral content increased exponentially across the calcified cartilage interface. These observations reveal new insights into both region- and age-dependent changes across the cartilage-to-bone junction and will serve as critical benchmark parameters for current efforts in integrative cartilage repair.

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Section: Discussionsupporting

confidence: 92%

FTIR-I Compositional Mapping of the Cartilage-to-Bone Interface as a Function of Tissue Region and Age

Khanarian

Boushell

Spalazzi

et al. 2014

Journal of Bone and Mineral Research

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“…suggesting higher turnover. This is also in agreement with other findings of our labo ratory where an increased EDTA extractability was observed in spongy bone relative to compact bone [11] and also with the finding in the literature [ 10]. The analysis of the fractionated bone particles showed a decrease in extractability and matrix size according to the increase in mineralization.…”

Section: Discussionsupporting

confidence: 93%

Collagen and Non-Collagenous Proteins in Different Mineralization Stages of Human Femur

Mbuyi-Muamba

Dequeker

Gevers³

1989

Cells Tissues Organs

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Mineralized tissues exhibit varying degrees of mineralization in different areas within the same bone. Using the technique of density gradient fractionation, bone powder from the diaphysis of human femur has been separated in different fractions corresponding to the degree of mineralization. Isolated bone fractions were analysed for their content in collagen and non-collagenous proteins. The results showed marked differences between compact and spongy bones, this latter containing higher proportions of little mineralized bone particles than the former (p < 0.01).The ethylenediaminetetra-acetic acid (EDTA) extractability and the bone matrix size decreased relative to the decrease in specific gravity of bone particles. Among the matrix components of different fractions, sialoprotein consistently increased with the increase in specific gravity while proteoglycan decreased in reverse manner to the increase in collagen. However, in the most mineralized fraction (specific gravity: 2.33 g/cm³), the proteoglycan amount increased while collagen decreased. In conclusion this study of bone maturation in human femur confirms the suitability of the technique of density gradient fractionation in the studies of bone matrix-mineral interactions. Apart from the fraction with the highest specific gravity, the analytical results obtained in fractions are similar to those observed in age-related bone changes, suggesting that the increase in mineralization degree of bone particles may be related to their age.

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“…Alterations in biochemical composition of these bones may affect the latter properties and thus the mechanical behavior of the bones (Burstein et al 1977). Earlier studies have shown marked differences between cortical and trabecular bone composition (Mbuyi-Muamba & Dequeker 1987, Ninomiya et al 1990). This is not really surprising, seeing the gross morphologic differences between these two bone types.…”

Section: Discussionmentioning

confidence: 99%

Variations in trabecular bone composition with anatomical site and age: potential implications for bone quality assessment

Aerssens¹,

Boonen²,

Joly³

et al. 1997

Journal of Endocrinology

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Skeletal site-related differences in trabecular bone composition have been studied in autopsy samples from 63 individuals (age range 23-92 years). From each individual, bone samples were excised from the iliac crest, lumbar spine, femoral neck, and calcaneus. Samples were analyzed for their content of ash, calcium, collagen, extractable proteins, osteocalcin, and IGF-I. Significant differences were found between the skeletal sites, the lumbar spine being the least mineralized site and the femur the most. The femur and lumbar spine had a higher osteocalcin and IGF-I content compared with the other skeletal sites, suggesting a higher bone turnover rate. The intercorrelations between the anatomical sites were low for minerals and collagen but high for osteocalcin and IGF-I. The latter might indicate that the presence of these proteins in the bone matrix is mainly controlled by endocrine mechanisms which may influence the osteoblast function. Finally, regression analysis showed a significant age-related decrease of skeletal IGF-I at all sites examined. This finding supports the hypothesis of an IGF-I-mediated pathogenesis of senile osteoporosis. In summary, our data imply that a global assessment of skeletal function and bone quality, based upon analyses at one anatomical site, should be applied with caution.

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Biochemical Anatomy of Human Bone: Comparative Study of Compact and Spongy Bone in Femur, Rib and Iliac Crest

Cited by 18 publications

References 7 publications

FTIR-I Compositional Mapping of the Cartilage-to-Bone Interface as a Function of Tissue Region and Age

FTIR-I Compositional Mapping of the Cartilage-to-Bone Interface as a Function of Tissue Region and Age

Collagen and Non-Collagenous Proteins in Different Mineralization Stages of Human Femur

Variations in trabecular bone composition with anatomical site and age: potential implications for bone quality assessment

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