Biochemical analysis of three putative KaiC clock proteins from Synechocystis sp. PCC 6803 suggests their functional divergence

Wiegard, Anika; Dörrich, Anja K.; Deinzer, Hans-Tobias; Beck, Christian; Wilde, Annegret; Holtzendorff, Julia; Axmann, Ilka M.

doi:10.1099/mic.0.065425-0

Cited by 48 publications

(70 citation statements)

References 59 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it has been proposed that the kaiA kaiB1 kaiC1 locus of Synechocystis sp. 6803 plays a critical role in establishing circadian rhythmicity, as has been demonstrated in S. elongatus PCC 7942, and that kaiB3 and kaiC3 may provide fine-tuning (93,94).…”

Section: Circadian Systems In Other Cyanobacteriamentioning

confidence: 99%

Circadian Rhythms in Cyanobacteria

Cohen

Golden

2015

Microbiol Mol Biol Rev

238

224

View full text Add to dashboard Cite

SUMMARYLife on earth is subject to daily and predictable fluctuations in light intensity, temperature, and humidity created by rotation of the earth. Circadian rhythms, generated by a circadian clock, control temporal programs of cellular physiology to facilitate adaptation to daily environmental changes. Circadian rhythms are nearly ubiquitous and are found in both prokaryotic and eukaryotic organisms. Here we introduce the molecular mechanism of the circadian clock in the model cyanobacteriumSynechococcus elongatusPCC 7942. We review the current understanding of the cyanobacterial clock, emphasizing recent work that has generated a more comprehensive understanding of how the circadian oscillator becomes synchronized with the external environment and how information from the oscillator is transmitted to generate rhythms of biological activity. These results have changed how we think about the clock, shifting away from a linear model to one in which the clock is viewed as an interactive network of multifunctional components that are integrated into the context of the cell in order to pace and reset the oscillator. We conclude with a discussion of how this basic timekeeping mechanism differs in other cyanobacterial species and how information gleaned from work in cyanobacteria can be translated to understanding rhythmic phenomena in other prokaryotic systems.

show abstract

Section: Circadian Systems In Other Cyanobacteriamentioning

confidence: 99%

Circadian Rhythms in Cyanobacteria

Cohen

Golden

2015

Microbiol Mol Biol Rev

238

224

View full text Add to dashboard Cite

show abstract

“…The pUR self-replicating vector was used to express SynEtr1 in the SynEtr1DTM2 background (Wiegard et al, 2013). The pUR vector confers both kanamycin and spectinomycin resistance.…”

Section: Generation and Confirmation Of Synetr1 Disruption Deletionmentioning

confidence: 99%

Ethylene Regulates the Physiology of the Cyanobacterium Synechocystis sp. PCC 6803 via an Ethylene Receptor

2016

View full text Add to dashboard Cite

Ethylene is a plant hormone that plays a crucial role in the growth and development of plants. The ethylene receptors in plants are well studied, and it is generally assumed that they are found only in plants. In a search of sequenced genomes, we found that many bacterial species contain putative ethylene receptors. Plants acquired many proteins from cyanobacteria as a result of the endosymbiotic event that led to chloroplasts. We provide data that the cyanobacterium Synechocystis (Synechocystis sp. PCC 6803) has a functional receptor for ethylene, Synechocystis Ethylene Response1 (SynEtr1). We first show that SynEtr1 directly binds ethylene. Second, we demonstrate that application of ethylene to Synechocystis cells or disruption of the SynEtr1 gene affects several processes, including phototaxis, type IV pilus biosynthesis, photosystem II levels, biofilm formation, and spontaneous cell sedimentation. Our data suggest a model where SynEtr1 inhibits downstream signaling and ethylene inhibits SynEtr1. This is similar to the inverse-agonist model of ethylene receptor signaling proposed for plants and suggests a conservation of structure and function that possibly originated over 1 billion years ago. Prior research showed that SynEtr1 also contains a light-responsive phytochrome-like domain. Thus, SynEtr1 is a bifunctional receptor that mediates responses to both light and ethylene. To our knowledge, this is the first demonstration of a functional ethylene receptor in a nonplant species and suggests that that the perception of ethylene is more widespread than previously thought.

show abstract

“…В дополнение к генам kai, объединенным в кластер, у некоторых цианобактерий встречаются гены kaiB и kaiC, рассеянные по геному. Будучи гомологичными своим «кластерным» аналогам, эти гены филогенетически и функционально дивергировали (Dvornyk, Knudsen, 2005) и, вероятно, более не связаны с циркадной системой (Wiegard et al, 2013).…”

Section: циркадная система цианобактерий: обзорunclassified

“…Такие копии этих генов по-прежнему присутствуют у некоторых цианобактерий (например, Synechocystis sp. РСС 6803), существенно отличаясь от своих истинных аналогов (Dvornyk et al, 2003;Dvornyk, Knudsen, 2005;Wiegard et al, 2013). Оперон kaiBC возник у цианобактерий приблизительно около 2,300 млрд лет назад и затем горизонтально был перенесен в другие прокариоты (Dvornyk et al, 2003).…”

Section: эволюционная история циркадной системы цианобактерийunclassified

“…Точно так же, по результатам анализа сдвига скорости эволюции, было высказано предположение, что разъединенные гены kaiB и kaiC, встречающиеся в геномах некоторых цианобактерий, не имеют циркадной функции (Dvornyk, Knudsen, 2005). Это недавно было продемонстрировано in vitro в экспериментах по фосфорилированию KaiC (Wiegard et al, 2013).…”

Section: исследования эволюции циркадной системы цианобактерий в эпохunclassified

See 1 more Smart Citation

Evolution of the circadian clock system in cyanobacteria: a genomic perspective

Dvornyk¹

2016

Algologia

View full text Add to dashboard Cite

ВведениеЦиркадные ритмы, или так называемые «внутренние часы», возникли в клетках живых организмов как основной инструмент адаптации к смене дня и ночи, вызванной вращением нашей планеты вокруг своей оси (Pittendrigh, 1993;Dunlap et al., 2004). Этот механизм контролирует свое-временную экспрессию значительной части генов в геноме.

show abstract

Biochemical analysis of three putative KaiC clock proteins from Synechocystis sp. PCC 6803 suggests their functional divergence

Cited by 48 publications

References 59 publications

Circadian Rhythms in Cyanobacteria

Circadian Rhythms in Cyanobacteria

Ethylene Regulates the Physiology of the Cyanobacterium Synechocystis sp. PCC 6803 via an Ethylene Receptor

Evolution of the circadian clock system in cyanobacteria: a genomic perspective

Contact Info

Product

Resources

About