Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion's surface

The Use of 3' Non-coding Nucleotide Sequences in the Taxonomy of Potyviruses: Application to Watermelon Mosaic Virus 2 and Soybean Mosaic Virus-N

“…We have previously commented (Shukla et al, 1986(Shukla et al, , 1988cShukla & Ward, 1988) on the close structural relationship between the coat proteins of PeMV and strains of PVY, and the possibility that PeMV is a strain of PVY. Comparison of the 3' untranslated region of PeMV (Dougherty et al, 1985) with that published for PVY-I (Rosner & Raccah, 1988) shows that the 3' untranslated sequences are 92% homologous (Table 1), supporting our contention that they are closely related genetically and should be considered strains of one virus. In a more recent paper, van der Vlugt et al (1989) have determined the nucleic acid sequence of the coat proteincoding and 3' untranslated region of PVY-N and have reached the same conclusion.…”

A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication

“…Conformational changes are anticipated when the socalled neutral Gly is replaced by charged, hydrophilic or hydrophobic amino acids. In the NAT isolates, Gly is replaced by a charged aa [Gly to Glu in TVMV (Atreya et al, 1990); Gly to Asp in soybean mosaic virus (Jayaram et al, 1991)], by a polar aa [Gly to Ser in tobacco etch virus (TEV) (Allison et al, 1985)] or by a hydrophobic aa [Gly to Leu in plum pox virus (Maiss et al, 1989)]. The two explanations suggested above may also hold true for ZYMV, but testing them will require the insertion of additional mutations and deletions in CP and a better understanding of the relationships between CP and HC.…”

cDNA cloning and sequence analysis of the 3'-terminal region of zucchini yellow mosaic virus RNA

“…This problem has been observed for several other potyviral coat proteins (Shukla et al, 1988; Allison et al, 1985a;Domier et al, 1986), particularly those beginning with S rather than G or A (Shukla et al, 1988). The predicted Mr (approx.…”

“…The first region is potyvirus-specific (e.g. the N-terminal region of ZYMV shares less than 12~ homology with other potyviral N-terminal regions), is subject to removal by limited prote01ysis, is externally located, and is the primary antigenic determinant Dougherty et al, 1985;Allison et al, 1985a). Removal of this region does not interfere with the apparent structure or infectivity, and so presumably the RNA binding capacity and coat proteincoat protein interactions involved in viral assembly lie downstream within the highly conserved central and Cterminal regions characteristic of potyviral coat proteins (Dougherty et al, 1985;.…”