Biochemical analysis of respiratory metabolism in the heterofermentative<i>Lactobacillus spicheri</i>and<i>Lactobacillus reuteri</i>

Ianniello, Rocco Gerardo; Zheng, Jinshui; Zotta, Teresa; Ricciardi, Annamaria; Gänzle, Michael G.

doi:10.1111/jam.12853

Cited by 30 publications

(61 citation statements)

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“…Ianniello et al . () demonstrated the over‐expression of pox and the repression of pdh in aerobic and respiratory cells of Lactobacillus rhamnosus , confirming that POX primarily mediates the acetate production in the presence of oxygen, while PDH, being expressed in anaerobic condition, may contribute to the acetate formation in anaerobic cultures. More recently, Ianniello et al .…”

Section: Discussionmentioning

confidence: 70%

“…, ; Ianniello et al . ), not properly suitable for metabolic investigations, or in CDM that poorly supported the growth in the presence of oxygen (Teusink et al . ; Saguir and de Nadra ; Wegkamp et al .…”

Section: Discussionmentioning

confidence: 99%

“…; Ianniello et al . ), shifting the pyruvate metabolism from lactate to acetate production because of pyruvate oxidase (POX) and acetate kinase (ACK) activities. The substrate level phosphorylation (POX/ACK) in aerobic condition and the synthesis of components of a respiratory chain, including cytochrome bd oxidase, moreover, contribute to energy gain (through extra ATP generation) and depletion of intracellular oxygen (lower oxidative stress), promoting higher growth rates and final cell densities (Lechardeur et al .…”

Section: Introductionmentioning

confidence: 99%

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Tween 80 and respiratory growth affect metabolite production and membrane fatty acids inLactobacillus caseiN87

et al. 2017

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tween 80 and respiratory growth affect metabolite production and membrane fatty acids inLactobacillus caseiN87

et al. 2017

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“…Both species show the typical heterofermentative pattern of metabolic genes, i.e., the absence of aldolase and phosphofructokinase and the presence of aad2 and mdh. Metabolite analysis confirmed that L. spicheri is a heterofermentative organism (79).…”

Section: Figmentioning

confidence: 69%

A Genomic View of Lactobacilli and Pediococci Demonstrates that Phylogeny Matches Ecology and Physiology

Zheng

Ruan

Sun

et al. 2015

Appl Environ Microbiol

204

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cLactobacilli are used widely in food, feed, and health applications. The taxonomy of the genus Lactobacillus, however, is confounded by the apparent lack of physiological markers for phylogenetic groups of lactobacilli and the unclear relationships between the diverse phylogenetic groups. This study used the core and pan-genomes of 174 type strains of Lactobacillus and Pediococcus to establish phylogenetic relationships and to identify metabolic properties differentiating phylogenetic groups. The core genome phylogenetic tree separated homofermentative lactobacilli and pediococci from heterofermentative lactobacilli. Aldolase and phosphofructokinase were generally present in homofermentative but not in heterofermentative lactobacilli; a twodomain alcohol dehydrogenase and mannitol dehydrogenase were present in most heterofermentative lactobacilli but absent in most homofermentative organisms. Other genes were predominantly present in homofermentative lactobacilli (pyruvate formate lyase) or heterofermentative lactobacilli (lactaldehyde dehydrogenase and glycerol dehydratase). Cluster analysis of the phylogenomic tree and the average nucleotide identity grouped the genus Lactobacillus sensu lato into 24 phylogenetic groups, including pediococci, with stable intra-and intergroup relationships. Individual groups may be differentiated by characteristic metabolic properties. The link between phylogeny and physiology that is proposed in this study facilitates future studies on the ecology, physiology, and industrial applications of lactobacilli. Lactobacilli are significant members of animal and human microbiota and of the plant phyllosphere. Owing to their stable association with humans as well as raw material for food production, lactobacilli also occur in many or most food fermentations. Lactobacilli are at the interface of aerobic and anaerobic life. Many lactobacilli retain the conditional capacity for respiration (1), but their ecology and physiology are mainly related to the fermentative conversion of sugars to organic acids (2, 3). Lactobacilli employ the Embden-Meyerhof pathway (glycolysis) and/or the phosphoketolase pathway for conversion of hexoses (2). These pathways have a low energetic efficiency; lactobacilli compensate for this disadvantage by rapid depletion of carbon sources and by accumulation of organic acids to inhibit competitors. The evolution and ecology of lactobacilli are shaped by niche adaptation and reduction of genome size (4). Many species, e.g., Lactobacillus plantarum, maintain a genetic diversity that enables occupation of diverse ecological niches (5). Other species are highly specialized and reduce their genomes more extensively. Examples include the insect-associated Lactobacillus fructivorans (6); Lactobacillus iners, a species specialized for the female human urogenital tract (7); and Lactobacillus reuteri, a host-specific intestinal symbiont of vertebrate animals (8). Lactobacillus delbrueckii has undergone a very recent reduction of genome size to adapt to dairy fermentations ...

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“…cydA (encoding for the subunit I of cytochrome bd oxidase) expression has been studied by Duwat et al ([27], Northern blot analysis) and by Arioli et al ([28], qRT-PCR analysis) in Lc . lactis , and by Ianniello et al ([29], qRT-PCR analysis) in L . rhamnosus and L .…”

Section: Discussionmentioning

confidence: 99%

Investigation of Factors Affecting Aerobic and Respiratory Growth in the Oxygen-Tolerant Strain Lactobacillus casei N87

et al. 2016

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Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction. Respiration increased biomass production compared to anaerobiosis and unsupplemented aerobiosis, and altered the central metabolism rerouting pyruvate away from lactate accumulation. All enzymatic activities, except lactate dehydrogenase, were higher in respiratory cultures, while unsupplemented aerobiosis with 60% of DO promoted H2O2 and free radical accumulation. Respiration improved the survival to oxidative and freeze-drying stresses, while significant numbers of dead, damaged and viable but not cultivable cells were found in unsupplemented aerobic cultures (60% DO). Analysis of gene expression suggested that the activation of aerobic and respiratory pathways occurred during the exponential growth phase, and that O2 and hemin induced, respectively, the transcription of pox and cydABCD genes. Respiratory cultivation might be a natural strategy to improve functional and technological properties of L. casei.

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Biochemical analysis of respiratory metabolism in the heterofermentativeLactobacillus spicheriandLactobacillus reuteri

Abstract: The exploitation of oxygen-tolerant phenotypes of Lact. spicheri may be useful for the development of improved starter cultures.

Cited by 30 publications

References 51 publications

Tween 80 and respiratory growth affect metabolite production and membrane fatty acids inLactobacillus caseiN87

Tween 80 and respiratory growth affect metabolite production and membrane fatty acids inLactobacillus caseiN87

A Genomic View of Lactobacilli and Pediococci Demonstrates that Phylogeny Matches Ecology and Physiology

Investigation of Factors Affecting Aerobic and Respiratory Growth in the Oxygen-Tolerant Strain Lactobacillus casei N87

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