Biochemical analysis of a recombinant glutathione transferase from the cestode Echinococcus granulosus

Auranofin-induced oxidative stress causes redistribution of the glutathione pool in Taenia crassiceps cysticerci

“…The first defensive line of these parasites is represent by glutathione peroxidase (GPx) [57][58][59] and peroxiredoxins (Prx) [60][61][62][63][64], which acts as ROS scavengers, as well as glutathione S-transferase (GST) [65][66][67], involved in the elimination of xenobiotic compounds. Both of these protective systems are dependent of the glutathione-reductase activity of the multifunctional TGR.…”

Purification of a recombinant glutathione transferase from the causative agent of hydatidosis, Echinococcus granulosus

“…Why is the purification factor greater than 1 and what does it mean? Under more controlled conditions, the specific activity (A s ) of the purified EgGST was 2.5 U/mg [13], how would you explain the differences with the experimental results?…”

“…The plasmid containing EgGST1 (O16058_ECHGR) [13] was kindly provided by Veronica Fernandez (Instituto de Higiene, Universidad de la Republica, vfernan@fq.edu.uy) who authorized us to distribute it upon request for teaching purposes. Protein expression was performed as reported in Harispe et al, 2010 [13] with slight modifications.…”

“…Protein expression was performed as reported in Harispe et al, 2010 [13] with slight modifications. The transformed bacteria from BL21(DE3) strain were grown overnight in LB (Luria Bertani) medium with kanamycin 50 mg/mL at 378C and shaking at 200 rpm.…”

“…In helminthes, glutathione transferases (GST) are thought to be the most important xenobiotic detoxification system and may contribute to resilience factors in parasites such as E. granulosus [12][13][14]. GST catalyze the nucleophilic attack of glutathione (GSH) on an electrophile [Eq.…”