Biochemical analyses of the crustacean hyperglycemic hormone of the crayfish Astacus leptodactylus

Kallen, Janine L.; Reijntjens, F.M.J.; Peters, Dorien J.M.; Herp, F. Van

doi:10.1016/0016-6480(86)90202-9

Cited by 14 publications

(3 citation statements)

References 28 publications

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“…The purified fractions were tested in a bioassay (according to Leuven et ul.. 1982), and immunoreactivity was tested by a direct ELISA using the anti-Astacus-CHH serum mentioned above. The resulting purified material was a biologically and immunologically active peptide with a MW of around 6500 Da, which is in agreement with former results (e.g., Keller, 1977;Kallen et at., 1986). Female Swiss mice received a first intraperitoneal injection comprising 5 ^g purified CHH material buffered with 0.03 M ammonium acetate, pH 8.5, and emulsified in Freund's complete ad-1 Results were presented at the 14th Conference of European Comparative Endocrinologists.…”

Section: Antiserasupporting

confidence: 92%

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Circadian Rhythmicity of the Crustacean Hyperglycemic Hormone (CHH) in the Hemolymph of the Crayfish

Kallen¹,

Abrahamse²,

Herp³

1990

The Biological Bulletin

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The crustacean hyperglycemic hormone (CHH) is involved in the regulation of endogenous blood glucose metabolism. In this paper we describe the daily rhythmicity in the blood glucose and the blood CHH content of the crayfish Orconectes limosus. Both blood CHH and blood glucose levels increase during the first hours after the beginning of darkness. The bioactivity of released CHH is far higher than that of CHH stored in the sinus gland. Moreover, the released hyperglycemic material shows an affinity for high molecular weight proteins in the hemolymph. Preliminary results suggest that subunits of hemocyanin may act as potential carrier-proteins for bioactive CHH.

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Section: Antiserasupporting

confidence: 92%

“…Furthermore, limited research on the chemical nature of newly synthesized CHH points to the presence of a prohormone or precursor in the perikarya (Stuenkel, 1983: Van Wormhoudt et al. 1984aKallen et al, 1986). Weidemann el al.…”

Section: Discussionmentioning

confidence: 99%

Circadian Rhythmicity of the Crustacean Hyperglycemic Hormone (CHH) in the Hemolymph of the Crayfish

Kallen¹,

Abrahamse²,

Herp³

1990

The Biological Bulletin

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“…Biochemical characterization of CHH family neuropeptides began in the 1980s using chromatographic techniques, and CHH family members were restricted to CHH, MIH and GIH [ 34 , 35 , 36 ]. The hyperglycemic functions of the CHH have been reported in many decapods.…”

Section: Discussionmentioning

confidence: 99%

Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis

Shi

Zhou

et al. 2018

PLoS ONE

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The recent use of RNA-Seq to study the transcriptomes of different species has helped identify a large number of new genes from different non-model organisms. In this study, five distinctive transcripts encoding for neuropeptide members of the CHH/MIH/GIH family have been identified from the spermatophore transcriptome of the shrimp Fenneropenaeus merguiensis. The size of these transcripts ranged from 531 bp to 1771 bp. Four transcripts encoded different CHH-family subtype I members, and one transcript encoded a subtype II member. RT-PCR and RACE approaches have confirmed the expression of these genes in males. The low degree of amino acid sequence identity among these neuropeptides suggests that they may have different specific function(s). Results from a phylogenetic tree analysis indicated that these neuropeptides were likely derived from a common ancestor gene resulting from mutation and gene duplication. These CHH-family members could be grouped into distinct clusters, indicating a strong structural/functional relationship among these neuropeptides. Eyestalk removal caused a significant increase in the expression of transcript 32710 but decreases in expression for transcript 28020. These findings suggest the possible regulation of these genes by eyestalk factor(s). In summary, the results of this study would justify a re-evaluation of the more generalized and pleiotropic functions of these neuropeptides. This study also represents the first report on the cloning/identification of five CHH family neuropeptides in a non-neuronal tissue from a single crustacean species.

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Invertebrate Endocrinology

Landau

Biggers

Laufer

1997

Comprehensive Physiology

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The sections in this article are: Growth and Morphogenetic Factors Insects Crustaceans Molluscs Other Invertebrates Regenerative Factors Crustaceans Insects Cnidarians Platyhelminths Annelids Echinoderms Others Pigmentary Factors Crustaceans Insects Osmoregulatory Factors Factors that May Increase Osmotic Pressure Factors that May Decrease Osmotic Pressure Metabolic Factors Biogenic Amines JHs and Farnesylacetone Adipokinetic Hormones and RPCH Crustacean Hyperglycemic Hormones Other Metabolic Factors Reproductive Factors Flatworms, Nemertines, and Nematodes Molluscs Crustaceans Insects Other Arthropods Annelids Echinoderms Behavioral Factors In Vitro Studies In Vivo Studies Pheromones Summary

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Biochemical analyses of the crustacean hyperglycemic hormone of the crayfish Astacus leptodactylus

Cited by 14 publications

References 28 publications

Circadian Rhythmicity of the Crustacean Hyperglycemic Hormone (CHH) in the Hemolymph of the Crayfish

Circadian Rhythmicity of the Crustacean Hyperglycemic Hormone (CHH) in the Hemolymph of the Crayfish

Functional and evolutionary implications from the molecular characterization of five spermatophore CHH/MIH/GIH genes in the shrimp Fenneropenaeus merguiensis

Invertebrate Endocrinology

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