Biochemical alterations of<i>Candida albicans</i>during the phenotypic transition from yeast to hyphae captured by Fourier transform mid-infrared-attenuated reflectance spectroscopy

Shi, Qin Yin; Câmara, Cristiane Rodrigues Silva; Schlegel, Vicki

doi:10.1039/c8an01452c

Cited by 6 publications

(2 citation statements)

References 57 publications

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“…C. albicans reversibly switches among unicellular ovoid yeast and multicellular ellipsoid pseudohyphal and elongated hyphal morphologies (80). All three stages are capable of producing an extracellular matrix and adhering to host tissues.…”

Section: Discussionmentioning

confidence: 99%

Dectin-2-Targeted Antifungal Liposomes Exhibit Enhanced Efficacy

Ambati

Ellis

Lin

et al. 2019

mSphere

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Invasive fungal diseases caused by Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus have mortality rates ranging from 10 to 95%. Individual patient costs may exceed $100,000 in the United States. All antifungals in current use have serious limitations due to host toxicity and/or insufficient fungal cell killing that results in recurrent infections. Few new antifungal drugs have been introduced in the last 2 decades. Hence, there is a critical need for improved antifungal therapeutics. By targeting antifungal-loaded liposomes to α-mannans in the extracellular matrices secreted by these fungi, we dramatically reduced the effective dose of drug. Dectin-2-coated liposomes loaded with amphotericin B bound 50- to 150-fold more strongly to C. albicans, C. neoformans, and A. fumigatus than untargeted liposomes and killed these fungi more than an order of magnitude more efficiently. Targeting drug-loaded liposomes specifically to fungal cells has the potential to greatly enhance the efficacy of most antifungal drugs.

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Section: Discussionmentioning

confidence: 99%

Dectin-2-Targeted Antifungal Liposomes Exhibit Enhanced Efficacy

Ambati

Ellis

Lin

et al. 2019

mSphere

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“…The parameters used in this work are the same as those used formerly in [21] (frequency of 32 kHz and voltage The second derivative of the spectra in the mixed region revealed bands related to the chitin (1348 cm −1 , 1381 cm −1 , 1392 cm −1 and 1412 cm −1 ) [45] with greater intensities in the CAP and caspofungin groups, when compared to the negative control (Figure 6a). The bands at 1404 cm −1 (δ sym C(CH 3 ) 2 ) are related to proteins, carbohydrates, and lipids, whereas the band at 1455 cm −1 (δ CH 2 ) corresponds to lipids and proteins [46]. These bands' intensities were higher after treatment with CAP when compared to the caspofungin and the negative control.…”

Section: Plasma Sourcementioning

confidence: 95%

Helium Cold Atmospheric Plasma Causes Morphological and Biochemical Alterations in Candida albicans Cells

Rovetta-Nogueira,

Borges,

Oliveira Filho

et al. 2023

Molecules

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(1) Background: Previous studies reported the promising inhibitory effect of cold atmospheric plasma (CAP) on Candida albicans. However, the exact mechanisms of CAP’s action on the fungal cell are still poorly understood. This study aims to elucidate the CAP effect on C. albicans cell wall, by evaluating the alterations on its structure and biochemical composition; (2) Methods: C. albicans cells treated with Helium-CAP were analyzed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in order to detect morphological, topographic and biochemical changes in the fungal cell wall. Cells treated with caspofungin were also analyzed for comparative purposes; (3) Results: Expressive morphological and topographic changes, such as increased roughness and shape modification, were observed in the cells after CAP exposure. The alterations detected were similar to those observed after the treatment with caspofungin. The main biochemical changes occurred in polysaccharides content, and an overall decrease in glucans and an increase in chitin synthesis were detected; (4) Conclusions: Helium-CAP caused morphological and topographic alterations in C. albicans cells and affected the cell wall polysaccharide content.

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Isotope labeled 3D-Raman confocal imaging and atomic force microscopy study on epithelial cells interacting with the fungus Candida albicans

Stanca,

Mogavero,

Fritzsche

et al. 2024

Nanomedicine: Nanotechnology, Biology and Medicine

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Biochemical alterations ofCandida albicansduring the phenotypic transition from yeast to hyphae captured by Fourier transform mid-infrared-attenuated reflectance spectroscopy

Abstract: The first study that applies FTIR spectroscopy to monitor the biochemical modification of Candida albicans during the transition from a benign yeast to virulent hyphae using multiple spectral analyses.

Cited by 6 publications

References 57 publications

Dectin-2-Targeted Antifungal Liposomes Exhibit Enhanced Efficacy

Dectin-2-Targeted Antifungal Liposomes Exhibit Enhanced Efficacy

Helium Cold Atmospheric Plasma Causes Morphological and Biochemical Alterations in Candida albicans Cells

Isotope labeled 3D-Raman confocal imaging and atomic force microscopy study on epithelial cells interacting with the fungus Candida albicans

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