Biochemical activity and multiple locations of particulate guanylate cyclase in Rhyacophila dorsalis acutidens (Insecta: Trichoptera) provide insights into the cGMP signalling pathway in Malpighian tubules

Secca, Teresa; Sciaccaluga, Miriam; Marra, Andrea; Barberini, Lanfranco; Bicchierai, Maria Clara

doi:10.1016/j.jinsphys.2011.02.002

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…Effects of inhibitors of second messenger systems on the actions of cGMP and AedesCAPA-PVK-1 Intracellular levels of cGMP can be modulated by soluble guanylate cyclase which can be dependent on production of nitric oxide by nitric oxide synthase or by particulate quanylate cyclase which can be dependent on the activity of G proteins (see Derbyshire and Marletta, 2012;Secca et al, 2011). In other insect Malpighian tubules and adult A. aegypti, the NOS/NO pathway has been implicated in CAPA peptide function.…”

Section: Effect Of Cgmp On Basal Fluid Secretion Ratesmentioning

confidence: 99%

AedesCAPA-PVK-1 displays diuretic and dose dependent antidiuretic potential in the larval mosquito Aedes aegypti (Liverpool)

Ionescu

Donini

2012

Journal of Insect Physiology

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Section: Effect Of Cgmp On Basal Fluid Secretion Ratesmentioning

confidence: 99%

AedesCAPA-PVK-1 displays diuretic and dose dependent antidiuretic potential in the larval mosquito Aedes aegypti (Liverpool)

Ionescu

Donini

2012

Journal of Insect Physiology

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“…The tubule, as a major osmoregulatory tissue with a wide range of transporters in common with mammalian kidney [12], [21], [22], [23], responds to desiccation stress, salt stress [24], [25], oxidative stress [26], [27], [28], immune stress [29], and plays a key role in the defence against xenobiotics [30], [31], [32], [33], [34]. It is also a model for neuroendocrine control [35], [36], [37]. The Drosophila Malpighian tubule thus offers a particularly useful, and broadly relevant, system in which to address issues of asymmetry.…”

Section: Introductionmentioning

confidence: 99%

Functional Correlates of Positional and Gender-Specific Renal Asymmetry in Drosophila

et al. 2012

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BackgroundIn humans and other animals, the internal organs are positioned asymmetrically in the body cavity, and disruption of this body plan can be fatal in humans. The mechanisms by which internal asymmetry are established are presently the subject of intense study; however, the functional significance of internal asymmetry (outside the brain) is largely unexplored. Is internal asymmetry functionally significant, or merely an expedient way of packing organs into a cavity?Methodology/Principal FindingsLike humans, Drosophila shows internal asymmetry, with the gut thrown into stereotyped folds. There is also renal asymmetry, with the rightmost pair of renal (Malpighian) tubules always ramifying anteriorly, and the leftmost pair always sitting posteriorly in the body cavity. Accordingly, transcriptomes of anterior-directed (right-side) and posterior-directed (left-side) Malpighian (renal) tubules were compared in both adult male and female Drosophila. Although genes encoding the basic functions of the tubules (transport, signalling) were uniformly expressed, some functions (like innate immunity) showed positional or gender differences in emphasis; others, like calcium handling or the generation of potentially toxic ammonia, were reserved for just the right-side or left-side tubules, respectively. These findings correlated with the distinct locations of each tubule pair within the body cavity. Well known developmental genes (like dorsocross, dachshund and doublesex) showed continuing, patterned expression in adult tubules, implying that somatic tissues maintain both left-right and gender identities throughout life. Gender asymmetry was also noted, both in defence and in male-specific expression of receptors for neuropeptide F and sex-peptide: NPF elevated calcium only in male tubules.Conclusions/SignificanceAccordingly, the physical asymmetry of the tubules in the body cavity is directly adaptive. Now that the detailed machinery underlying internal asymmetry is starting to be delineated, our work invites the investigation, not just of tissues in isolation, but in the context of their unique physical locations and milieux.

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“…Consistent with this possibility, it is reported that stellate cells in the trichopteran insect Rhyacophila dorsalis acutidens, which has MTs that are morphologically similar to those of Drosophila, contain high levels of particulate guanylate cyclase activity (59). The hypothesized extracellular factor would act as a context-specific antidiuretic hormone, only reducing urine secretion in tubules that were stimulated by TA or kinin.…”

Section: Discussionmentioning

confidence: 76%

Inhibition of diuretic stimulation of an insect secretory epithelium by a cGMP-dependent protein kinase

Ruka

Miller

Blumenthal

2013

American Journal of Physiology-Renal Physiology

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The rate of urine secretion by insect Malpighian tubules (MTs) is regulated by multiple diuretic and antidiuretic hormones, often working either synergistically or antagonistically. In the Drosophila melanogaster MT, only diuretic factors have been reported. Two such agents are the biogenic amine tyramine (TA) and the peptide drosokinin (DK), both of which act on the stellate cells of the tubule to increase transepithelial chloride conductance. In the current study, TA and DK signaling was quantified by microelectrode recording of the transepithelial potential in isolated Drosophila MTs. Treatment of tubules with cGMP caused a significant reduction in the depolarizing responses to both TA and DK, while cAMP had no effect on these responses. To determine whether a specific cGMP-dependent protein kinase (PKG) was mediating this inhibition, PKG expression was knocked down by RNAi in either the principal cells or the stellate cells. Knockdown of Pkg21D in the stellate cells eliminated the modulation of TA and DK signaling. Knockdown of Pkg21D with a second RNAi construct also reduced the modulation of TA signaling. In contrast, knockdown of the expression of foraging or CG4839, which encodes a known and a putative PKG, respectively, had no effect. These data indicate that cGMP, acting through the Pkg21D gene product in the stellate cells, can inhibit signaling by the diuretic agents TA and DK. This represents a novel function for cGMP and PKG in the Drosophila MT and suggests the existence of an antidiuretic hormone in Drosophila.

show abstract

Biochemical activity and multiple locations of particulate guanylate cyclase in Rhyacophila dorsalis acutidens (Insecta: Trichoptera) provide insights into the cGMP signalling pathway in Malpighian tubules

Cited by 4 publications

References 58 publications

AedesCAPA-PVK-1 displays diuretic and dose dependent antidiuretic potential in the larval mosquito Aedes aegypti (Liverpool)

AedesCAPA-PVK-1 displays diuretic and dose dependent antidiuretic potential in the larval mosquito Aedes aegypti (Liverpool)

Functional Correlates of Positional and Gender-Specific Renal Asymmetry in Drosophila

Inhibition of diuretic stimulation of an insect secretory epithelium by a cGMP-dependent protein kinase

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