Biochar reduces containerized pepper blight caused by Phytophthora capsici

Abstract

Phytophthora blight caused by Phytophthora capsici is a serious disease affecting a wide range of plants. Biochar as a soil amendment could partially replace peat moss and has the potential to suppress plant diseases, but its effects on controlling phytophthora blight of container-grown peppers have less been explored, especially in combination of biological control using Trichoderma. In vitro (petri dish) and in vivo (greenhouse) studies were conducted to test sugarcane bagasse biochar (SBB) and mixed hardwood biochar (HB) controlling effects on pepper phytophthora blight disease with and without Trichoderma. Sugarcane bagasse biochar and HB were blended with the commercial substrate (CS, peat-based) at 10% (SBB10, by volume), and 10%, 30%, 50%, 70% (HB10, HB30, HB50, and HB70, by volume), respectively, and CS (CS100) was used as the control. Both in vitro and in vivo studies used randomized complete block design with three treatment factors: pathogen (without or with inoculation of P. capsici), biochar (different biochar treatments), and Trichoderma (without or with inoculation). In vitro results showed that Trichoderma inhibited P. capsici growth while biochar did not have significant beneficial effects. In vivo results showed that plants grown in HB30 and HB50 had similar or higher plant growth index and shoot dry weight than the control regardless of pathogen presence. In the presence of the pathogen, plants grown in HB30, HB50, and HB70 had significantly lower disease severity, and disease incidence ratings than the control, while Trichoderma did not show beneficial effects on controlling the disease. In conclusion, HB replacing 30% and 50% peat moss in substrate could reduce pepper blight disease caused by P. capsici without negatively affecting plant growth.