Biochar enhanced high-solid mesophilic anaerobic digestion of food waste: Cell viability and methanogenic pathways

Cui, Yuxuan; Mao, Feijian; Zhang, Jingxin; He, Yiliang; Tong, Yen Wah; Peng, Yinghong

doi:10.1016/j.chemosphere.2021.129863

Cited by 61 publications

(24 citation statements)

References 49 publications

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“…Other management options based on this principle have proven useful in achieving the full degradation of complex organic compounds, as it is the addition of biochar or the presence of bioanodes that favor different degradation pathways [106][107][108]. Cui et al [109] demonstrated the successful performance of the HSAD reactor when adding biochar, indicating that the improvement was mainly due to the presence of biochar in the media. This material could enhance food waste hydrolysis by promoting butyric acid degradation thanks to changes in community structures.…”

Section: Increasing Reactor Productivity By the Increase In Solid Content And Temperaturementioning

confidence: 99%

Biogas Production from Organic Wastes: Integrating Concepts of Circular Economy

Ellacuriaga

García-Cascallana

Gómez

2021

Fuels

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Anaerobic digestion is traditionally used for treating organic materials. This allows the valorization of biogas and recycling of nutrients thanks to the land application of digestates. However, although this technology offers a multitude of advantages, it is still far from playing a relevant role in the energy market and from having significant participation in decarbonizing the economy. Biogas can be submitted to upgrading processes to reach methane content close to that of natural gas and therefore be compatible with many of its industrial applications. However, the high installation and operating costs of these treatment plants are the main constraints for the application of this technology in many countries. There is an urgent need of increasing reactor productivity, biogas yields, and operating at greater throughput without compromising digestion stability. Working at organic solid contents greater than 20% and enhancing hydrolysis and biogas yields to allow retention times to be around 15 days would lead to a significant decrease in reactor volume and therefore in initial capital investments. Anaerobic digestion should be considered as one of the key components in a new economy model characterized by an increase in the degree of circularity. The present manuscript reviews the digestion process analyzing the main parameters associated with digestion performance. The novelty of this manuscript is based on the link established between operating reactor conditions, optimizing treatment capacity, and reducing operating costs that would lead to unlocking the potential of biogas to promote bioenergy production, sustainable agronomic practices, and the integration of this technology into the energy grid.

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Section: Increasing Reactor Productivity By the Increase In Solid Content And Temperaturementioning

confidence: 99%

Biogas Production from Organic Wastes: Integrating Concepts of Circular Economy

Ellacuriaga

García-Cascallana

Gómez

2021

Fuels

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“…3c shows the evolution of coenzyme F420 absorbance. Indeed, coenzyme F420 is a unique electron transport enzyme in the methane production pathway, and therefore coenzyme F420 is used to monitor methanogen activity to a certain extent [52]. Our results show that at the start of digestion, the coenzyme F420 activity of biochar-added runs are higher that the control.…”

Section: Evolution Of Ph Ammonia Nitrogen F420 Absorbance and Vfasmentioning

confidence: 72%

Enhancing thermophilic anaerobic co-digestion of sewage sludge and food waste with biogas residue biochar

Liu

Wang

Fang³

et al. 2022

Renewable Energy

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“…For sorting the live and dead microorganisms, the 2 mL treated samples were stained with 200 μL of 7-AAD (Thermo Fisher Scientific Inc., USA) for 5 min and were sorted on FCM according to the previous study. 19 Sorted dead and live bacterial solution samples were centrifuged at 5000g for 10 min and stored at −20 °C for microbial community analysis.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…In addition, flow cytometry (FCM) combined with fluorescence cytograms analysis divided the viable cells with complete membrane structures into bacteria with high DNA content 1 and low DNA content (LNA), which were considered active and inactive cells, respectively. 19,20 It was reported that a part of bacteria might be present in a viable but nonculturable state, 21 and the functional metabolic activities could be inaccurate to be analyzed based on total microorganisms at certain times. As impacted by membrane integrity on cell viability, the correlation between the viable bacteria and the efficiency of DIET via membrane-bound electron transport proteins remains unclear.…”

Section: ■ Introductionmentioning

confidence: 99%

Micro–Nano Magnetite-Loaded Biochar Enhances Interspecies Electron Transfer and Viability of Functional Microorganisms in Anaerobic Digestion

Chen

Zhang

et al. 2022

ACS Sustainable Chem. Eng.

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Magnetite-loaded biochar generated by gasification serves as a potential additive to facilitate electron transfer and boost methane production. In this study, the focus was placed on the mechanism of magnetite-loaded biochar-enhanced methanogens with live bacteria in real habitats, and the connection between live microorganisms and the methanogenic pathway was revealed. Magnetite-loaded biochar produced at a FeCl3-to-woodchip ratio (w/w) of 15/100 presented maximal methane production, where the daily methane yield was improved by 157% compared with the control. Magnetite accelerated the consumption of short-chain fatty acids through dissimilatory iron reduction, and it was converted into siderite and goethite after anaerobic digestion. The iron cycle coupling with the organic removal enhanced electron transfer efficiency and further transmitted electrons to promote methanogenesis. As indicated from the results, live high DNA (Atelge, M. R.; Atabani, A. E.; Banu, J. R.; Krisa, D.; Kaya, M.; Eskicioglu, C.; Kumar, G.; Lee, C.; Yildiz, Y. Ş.; Unalan, S.; et al. Fuel 2020, 270, 117494.) cells with high viability increased from 21 to 44%, which probably facilitated the electron transfer and direct interspecies electron transfer via membrane proteins. Moreover, the activities of the functional enzyme participating in the acetoclastic and hydrogenotrophic methanogenic pathways were enhanced. As revealed from the gene abundance from live microorganisms of the acetoclastic/hydrogenotrophic methanogenic pathway, the magnetite-loaded biochar facilitated the methanogenesis process, probably due to the DIET formed between the iron reduction bacteria Ruminococcaceae and methanogenesis Methanothrix and Methanosarcina by the magnetite-loaded biochar.

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Biochar enhanced high-solid mesophilic anaerobic digestion of food waste: Cell viability and methanogenic pathways

Cited by 61 publications

References 49 publications

Biogas Production from Organic Wastes: Integrating Concepts of Circular Economy

Biogas Production from Organic Wastes: Integrating Concepts of Circular Economy

Enhancing thermophilic anaerobic co-digestion of sewage sludge and food waste with biogas residue biochar

Micro–Nano Magnetite-Loaded Biochar Enhances Interspecies Electron Transfer and Viability of Functional Microorganisms in Anaerobic Digestion

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