Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method

Omoregie, Armstrong Ighodalo; Palombo, Enzo A.; Ong, Danny; Nissom, Peter Morin

doi:10.1016/j.conbuildmat.2019.116828

Cited by 86 publications

(54 citation statements)

References 56 publications

(102 reference statements)

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“…In general, their results have indicated that the rate of urea hydrolysis increases with increasing temperature, suggesting that the urease of most of the soil bacteria is not heat inactivated (up to the temperature ranging 45-60 °C). For an example, urease activity of Sporosarcina Pasteurii increased proportionally with temperature between 25 °C and 60 °C [24]. The similar tendency is reported for Pararhodobacter sp.…”

Section: Discussionsupporting

confidence: 78%

“…The enzymes of the bacteria are thermal sensitive and can readily be denatured by even a slight change of environmental temperature [22]. Most of the MICP studies have employed Sporosarcina pasteurrii for urea hydrolysis due to the highly active urease enzyme [5,6,11,13,[23][24][25]. Only a few MICP studies focused on introducing some other ureolytic strains including Bacillus sphaercus (MCP-11) [26], Pararhodobactor sp.…”

Section: Introductionmentioning

confidence: 99%

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Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

et al. 2019

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Microbial induced carbonate precipitation (MICP) is relatively an innovative soil improvement technique, learnt from the bio-mediated geochemical reactions that naturally occur in the earth surface. During the MICP, CaCO 3 is metabolically precipitated in soil pores, cement the particle contacts and improves the strength and stiffness of soil. Environment temperature is one of the most key factors that determines the efficiency MICP. The purpose of this study is to investigate the feasibility of stabilizing the slope soil of cold subarctic region (Hokkaido, Japan). The implication of MICP in cold subarctic zones remains as a major challenge, as the enzymatic performance of the bacteria typically declines during lower temperatures hence insufficient formation of CaCO 3 in soil matrix. Therefore, as a potential approach, this study attempted to investigate the feasibility of using the bacteria which have been adapted to native cold climatic conditions. The objectives of this paper are evaluating (1) the effect of temperature in bacterial response, and (2) the effect of grain size distribution in cementation mechanism. The observations suggest that the enzyme activity of the bacteria is negligible at and above 30 °C, whereas it is significant at relatively lower temperatures. The comparison of treated soils suggests that the fine content in slope soil increased number of particle contacts, facilitated effective packing, and promoted the effectiveness of MICP compared to that of uniformly graded sands. Finally, the technical feasibility in slope soil stabilization was well demonstrated using model solidification test. The limitations in stabilizing the slope are also discussed in detail.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

et al. 2019

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“…It can be observed that the calcite clusters that crystallized at particle contacts could enable the connectivity between soil particles, contributing as the primary source for strength and stability. Similar morphology of the calcium carbonate mineral could also be witnessed in other MICP-related works (Zhang et al 2017;Omoregie et al 2019). Moreover, there are crystals which were formed randomly on soil surfaces without subsidizing interparticle connection; by reducing the void spaces, however, those could secondarily contribute to the strength of the matrix (DeJong et al 2010).…”

Section: Effect Of Cementation Level On Durabilitysupporting

confidence: 73%

Durability analysis of bio-cemented slope soil under the exposure of acid rain

2021

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Purpose Instability of slope surface is a critical concern in Geotechnical and Environmental Engineering. MICP (Microbial-Induced Carbonate Precipitation), an innovative bio-cementation technique, has attracted the attention for slope surface protection. In this work, MICP was investigated to evaluate its durability under the exposure of acid rain and to advance the understanding on long-term performance of slope soil preserved by MICP. Methods MICP treatment was applied to a fine-grained slope soil using indigenous bacteria. Specimens treated to different cementation levels (% CaCO3) were exposed to acid rain (of varying pH) through two sorts of mechanisms: (i) infiltration and (ii) immersion. The evaluations were based on corrosion of CaCO3, mass loss, needle penetration tests, and scanning electron microscopy. Results The decrease in pH increased the corrosion of CaCO3, resulting in considerable loss in aggregate and unconfined compressive strength. However, increased cementation level showed high durability of specimens. The soils treated to 12.5% CaCO3 showed 19.9% soil loss, whereas it was reduced to 5.4% when cemented to 22.5% CaCO3. The results also revealed that the contact time of acid rain significantly governed the rate of corrosion, i.e., specimens subjected to lower infiltration rate (20 mm/h) showed higher loss of mass compared to that of higher rate (100 mm/h). Conclusion The long-term performance of MICP treatment is determined by (i) cementation level, (ii) pH, and (iii) infiltration rate of acid rain. High cementation level promotes the longevity of the treatment. Therefore, MICP to higher cementation level is recommended for long-term preservation of slope surface.

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“…Food or technical grade chemicals have successfully been tested for S. pasteurii cultivation. Their application would additionally reduce costs 21 , 42 . For large scale industrial applications, media preparation and sterilization must also be considered for cost calculations 20 .…”

Section: Resultsmentioning

confidence: 99%

Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media

Lapierre

Schmid

Ederer

et al. 2020

Sci Rep

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Microbial induced calcite precipitation (MICP) based on ureolysis has a high potential for many applications, e.g. restoration of construction materials. The gram-positive bacterium Sporosarcina pasteurii is the most commonly used microorganism for MICP due to its high ureolytic activity. However, Sporosarcina pasteurii is so far cultivated almost exclusively in complex media, which only results in moderate biomass concentrations at the best. Cultivation of Sporosarcina pasteurii must be strongly improved in order to make technological application of MICP economically feasible. The growth of Sporosarcina pasteurii DSM 33 was boosted by detecting auxotrophic deficiencies (L-methionine, L-cysteine, thiamine, nicotinic acid), nutritional requirements (phosphate, trace elements) and useful carbon sources (glucose, maltose, lactose, fructose, sucrose, acetate, L-proline, L-alanine). These were determined by microplate cultivations with online monitoring of biomass in a chemically defined medium and systematically omitting or substituting medium components. Persisting growth limitations were also detected, allowing further improvement of the chemically defined medium by the addition of glutamate group amino acids. Common complex media based on peptone and yeast extract were supplemented based on these findings. Optical density at the end of each cultivation of the improved peptone and yeast extract media roughly increased fivefold respectively. A maximum OD600 of 26.6 ± 0.7 (CDW: 17.1 ± 0.5 g/L) was reached with the improved yeast extract medium. Finally, culture performance and media improvement was analysed by measuring the oxygen transfer rate as well as the backscatter during shake flask cultivation.

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Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method

Abstract: Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method

Cited by 86 publications

References 56 publications

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

Durability analysis of bio-cemented slope soil under the exposure of acid rain

Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media

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