Biocatalytic Reactions in Ionic Liquids

Klembt, Sandra; Dreyer, Susanne; Eckstein, Marrit; Kragl, Udo

doi:10.1002/9783527621194.ch8

Cited by 9 publications

(15 citation statements)

References 141 publications

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“…During the last few years, ILs have been applied for biopurification and bio-extraction processes, and advantages, such as the improvement of enzyme stability, substrate and/or product selectivity, and suppression of side reactions were observed [20][21][22][23][24][25]. Moreover, IL-based ATPS are substantially less viscous than typical polymer-based ATPS [26,27].…”

Section: Introductionmentioning

confidence: 98%

Design of ionic liquids for lipase purification

Ventura

Sousa

Freire

et al. 2011

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 98%

Design of ionic liquids for lipase purification

Ventura

Sousa

Freire

et al. 2011

Journal of Chromatography B

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“…Indeed, during the last years, several ILs have been applied for biotransformation processes and advantages such as improvement of enzyme stability, substrate and/or product selectivity and suppression of side reactions could be observed (Klembt et al, 2007;Kragl et al, 2002;Park and Kazlauskas, 2003;van Rantwijk and Sheldon, 2007;van Rantwijk et al, 2003;Welton, 1999). The use of ILs as solvents for chemical reactions also revealed their excellent physicochemical properties: they dissolve both polar and non-polar organic, inorganic and polymeric materials (Dupont et al, 2002;Earle and Seddon, 2000;Holbrey and Seddon, 1999;OlivierBourbigou and Magna, 2002;Sheldon, 2001;Wasserscheid and Keim, 2000;Welton, 1999 6 ] have successfully been applied to increase the low solubility of sugars and to maintain the enzymatic activity in lipase-catalyzed synthesis of fatty acid sugar esters (Ganske and Bornscheuer, 2005a,b).…”

Section: Introductionmentioning

confidence: 99%

Ionic liquids for aqueous two‐phase extraction and stabilization of enzymes

Dreyer

Kragl

2007

Biotech & Bioengineering

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204

134

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The ionic liquid (IL) Ammoeng110 contains cations with oligoethyleneglycol units and was found to be highly effective for the formation of aqueous two-phase systems (ATPS) that can be used for the biocompatible purification of active enzymes. Above critical concentrations of the IL and an inorganic salt in aqueous solution, phase separation takes place resulting in the formation of an IL-enriched upper and a salt-enriched lower phase. For the optimization of the composition of IL-based ATPS with regard to the extraction of catalytically active enzymes, the Box-Wilson method of experimental design was successfully applied; IL-based ATPS proved to be suitable for the purification and stabilization of two different alcohol dehydrogenases (from Lactobacillus brevis and a thermophilic bacterium). Both enzymes were enriched in the IL-containing upper phase resulting in an increase of specific activity by a factor of 2 and 4 respectively. Furthermore, the presence of IL within the system provided the opportunity to combine the extraction process with the performance of enzyme-catalyzed reactions. The IL was found to exhibit a stability improving effect on both enzymes and a solubility enhancing effect on hydrophobic substrates. Thus the conversion and volumetric productivity of ADH catalyzed reduction of acetophenone could be increased significantly.

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“…When enzymes are used in either pure organic solvents or ionic liquids, the minimum amount of water, which is best described by the water activity, is of crucial importance to maintain the enzyme activity. For ionic liquids, the same methods can be used to maintain a constant water activity as those established for organic solvents [33]. However, in some ionic liquids (e.g.…”

Section: Effect Of Physicochemical Properties Of Ionic Liquids On Enzmentioning

confidence: 97%

“…esterification, kinetic resolution, reductions, oxidations hydrolysis, etc.) have been tested in monophasic system based on ionic liquid [14,15,33] 6 ], etc.) act as suitable reaction media for enzymatic catalysis in the same conditions.…”

Section: Enzymes In Ionic Liquidsmentioning

confidence: 99%

Compatibility of Ionic Liquids with Enzymes

Lan

Koo

2013

Production of Biofuels and Chemicals With Ionic Liquids

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The potential of ionic liquids as a green alternative to environmentally harmful volatile organic solvents has been well recognized. Being considered as "designer solvents", ionic liquids have been used extensively in a wide range of applications including biotransformations. As compared to those in traditional organic solvents, enzyme performance in ionic liquids is showed enhance in their activity, enantioselectivity, stability, as well as their recoverability and recyclability. This chapter will cover the biocompatibility issue of ionic liquids with enzymes. The effects of ionic liquid properties on the enzymatic reactions and conformation of enzyme as well as methods for activation and stabilization of enzymes in ionic liquids will be described. In addition, the current attempts for rational design of biocompatible ionic liquids will be also discussed.

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Biocatalytic Reactions in Ionic Liquids

Cited by 9 publications

References 141 publications

Design of ionic liquids for lipase purification

Design of ionic liquids for lipase purification

Ionic liquids for aqueous two‐phase extraction and stabilization of enzymes

Compatibility of Ionic Liquids with Enzymes

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