Biocatalytic production of useful esters by two forms of lipase from Candida rugosa

Linko, Yu‐Yen; Wu, Xiao Y.

doi:10.1002/(sici)1097-4660(199602)65:2<163::aid-jctb416>3.0.co;2-c

Cited by 48 publications

(23 citation statements)

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“…A lipase from Can. rugosa can catalyse ester synthesis by both esterification and transesterification (alcoholysis) under low-water conditions (Linko & Wu, 1996). Thus, the possibility of ester biosynthesis by lipases from dairy yeasts cannot be ruled out, given that many yeasts, including dairy yeasts, possess lipases (Hou, 1997;van den Tempel & Jakobsen, 2000;Addis et al, 2001;Guerzoni et al, 2001;Suzzi et al, 2001).…”

Section: Ester Biosynthesis By Dairy Yeastsmentioning

confidence: 99%

Esters and their biosynthesis in fermented dairy products: a review

Liu

Holland

Crow

2004

International Dairy Journal

329

269

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Section: Ester Biosynthesis By Dairy Yeastsmentioning

confidence: 99%

Esters and their biosynthesis in fermented dairy products: a review

Liu

Holland

Crow

2004

International Dairy Journal

329

269

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“…Many yeasts, such as Sporidiobolus pararoseus (Mase et al, 2011), Geotrichum sp. (Monfort et al, 1999), and Candida rugosa (Linko and Wu, 1996), have been reported to produce lipase. Of the many lipase-producing yeasts, C. rugosa is the most frequently used for commercial production of lipase.…”

Section: Fig 2 Enzymatic Properties Of Purified Lipase From Strain mentioning

confidence: 99%

Characterization and Application of Lipase 39-A from Cryptococcus flavescens for Cheese Flavoring

Mase

Asano

Ikeda

et al. 2013

FSTR

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“…In this sense, the genes for seven isoenzymes (Lip1-Lip7, 66% aminoacid identity and 84% similarity between them) were described in the early 90s [14]. Different purification methods have been described for the production of three isoenzymes, Lip1, Lip2 and Lip3 [10,[16][17][18][19][20][21][22][23]. Nevertheless, usually the commercial lipases from C. rugosa are composed by a mixture of Lip1 (PDB Code 1CRL [24,25]) and Lip3 (PDB Code 1LLF [26,27]) in different quantities and proportions among them [11,14,15,[28][29][30][31].…”

Section: Production Of Lipases In Fermentation Processes Induction Amentioning

confidence: 99%

Candida rugosa Lipase: A Traditional and Complex Biocatalyst

Marı́a¹,

Alcántara²,

Carballeira

et al. 2006

COC

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Different commercial preparations from Candida rugosa lipase lead often to an irreproducible behaviour when employed in slightly hydrated media, even when different samples from the same supplier are used. This conduct is triggered by several causes, such as the different concentration of the "real" catalyst in the different crude samples (quantity), or the inherent problems related to heterogeneous biocatalysts (i. e., different amount of water, diffusional problems, etc); furthermore, for C. rugosa lipase, the diverse percentage of different isoenzymes (quality) is another irreproducibility-inducing factor. In this sense, for a rational understanding of all the experimental data described for this complex biocatalyst, topics like the description of the role of the inducer on the fermentation, the biochemical characterisation of the crude biocatalysts and the establishment of synthetical strategies to overcome the irreproducibility problems must be covered. Once these tasks are fulfilled, we will be able to understand the relative catalytic activity of different samples from different origins, showing that enzymes should not merely be considered as a "white magic powder" with synthetical utility. Different techniques to evaluate the importance of the amount of water are discussed: 1 H-NMR for comparing the hydrolytic activity of isoenzymes, and sorption isotherms and Thermogravimetric and Differential Thermal Analysis (TGA/DTA) for understanding the esterification in organic media. Furthermore, different characterisation reactions useful for quantifying the lipase loading (heptyl oleate synthesis and transesterification of vinyl acetate with 1-heptanol) are proposed. Finally, a synthetical strategy based on the acylation, via vinyl acetate, of 1-heptanol, geraniol, nerol and cyclohexanol, for comparing the catalytical results and the isoenzymatic profile is discussed.

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Biocatalytic production of useful esters by two forms of lipase from Candida rugosa

Cited by 48 publications

References 0 publications

Esters and their biosynthesis in fermented dairy products: a review

Esters and their biosynthesis in fermented dairy products: a review

Characterization and Application of Lipase 39-A from Cryptococcus flavescens for Cheese Flavoring

Candida rugosa Lipase: A Traditional and Complex Biocatalyst

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