Biocatalytic portfolio of Basidiomycota

Schmidt‐Dannert, Claudia

doi:10.1016/j.cbpa.2016.01.002

Cited by 57 publications

(40 citation statements)

References 80 publications

(109 reference statements)

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“…The bias towards the Basidiomycota phylum is a reflection of the available genomic and transcriptomic data as Ascomycota species are comparatively understudied32. A total of 1046 canonical sequences were identified as predicted or confirmed hydrophobins.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision

Gandier

Langelaan

Won

et al. 2017

Sci Rep

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Class I hydrophobins are functional amyloids secreted by fungi. They self-assemble into organized films at interfaces producing structures that include cellular adhesion points and hydrophobic coatings. Here, we present the first structure and solution properties of a unique Class I protein sequence of Basidiomycota origin: the Schizophyllum commune hydrophobin SC16 (hyd1). While the core β-barrel structure and disulphide bridging characteristic of the hydrophobin family are conserved, its surface properties and secondary structure elements are reminiscent of both Class I and II hydrophobins. Sequence analyses of hydrophobins from 215 fungal species suggest this structure is largely applicable to a high-identity Basidiomycota Class I subdivision (IB). To validate this prediction, structural analysis of a comparatively distinct Class IB sequence from a different fungal order, namely the Phanerochaete carnosa PcaHyd1, indicates secondary structure properties similar to that of SC16. Together, these results form an experimental basis for a high-identity Class I subdivision and contribute to our understanding of functional amyloid formation.

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Section: Resultsmentioning

confidence: 99%

Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision

Gandier

Langelaan

Won

et al. 2017

Sci Rep

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“…Studying multipartner interactions provides new insight into chemical ecology and the natural products that mediate chemical communication or competition . In our previous work, induction of bioactive pigments in basidiomycetes by other fungi or bacteria appeared ubiquitous which incited further interests into this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, they are critical for global carbon cycling and to maintain forest ecosystems. To exert their ecological role, basidiomycetes have the capacity to produce diverse natural products [1,2]. Historically, research into these natural products has primarily been driven by drug development, crop plant protection purposes or biopolymer degradation [3].…”

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confidence: 99%

“…Following suit, generally multimodular and iteratively-acting enzymes catalyze these compounds, namely terpene synthases, polyketides synthases (PKSs) or nonribosomal peptide synthetases (NRPSs), respectively. Furthermore, it is a hallmark feature of natural products that they are often highly modified and functionalized [1]. Of particular note, many natural products feature chromophores [5] and some have been recognized as inducible by cocultures [6] or by physical injury [7]; to protect from ultraviolet (UV) light during symbiosis [8]; to deter insects [9]; and as iron reductants during lignocellulose breakdown [10], reviewed in refs [11][12][13].…”

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confidence: 99%

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Analysis of basidiomycete pigments in situ by Raman spectroscopy

Tauber

Matthäus

Lenz

et al. 2018

Journal of Biophotonics

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Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment production is triggered and pigment secretion is generated at the interaction zone. The identification and analysis of these pigments is important to understand the defense mechanism of fungi, which is essential to counteract an uncontrolled spread of harmful species. Usually, a detailed analysis of the pigments is time consuming as it depends on laborious sample preparation and isolation procedures. Furthermore, the applied protocols often influence the chemical integrity of the compound of interest. A possibility to noninvasively investigate the pigmentation is Raman microspectroscopy. The methodology has the potential to analyze the chemical composition of the sample spatially resolved at the interaction zone. After the acquisition of a representative spectroscopic library, the pigment production by basidiomycetes was monitored for during response to different fungi and bacteria. The presented results describe a very efficient noninvasive way of pigment analysis which can be applied with minimal sample preparation.

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“…One such source for the identification of enzymes with new and unusual activities are fungi. The increasing availability of fungal genome sequences, especially of Basidiomycota, gives access to a diverse, yet largely unexplored repertoire of oxidoreductases, oxygenases and hydrolases useful for biocatalysis (Schmidt‐Dannert, ). Other sources for new biocatalysts are microorganisms from extreme environments which have evolved robust enzymes that are active under harsh industrial process conditions (Ferrer et al ., ).…”

Section: Biocatalyst Developmentmentioning

confidence: 99%

A roadmap for biocatalysis – functional and spatial orchestration of enzyme cascades

Schmidt‐Dannert

López‐Gallego

2016

Microbial Biotechnology

116

100

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SummaryAdvances in biological engineering and systems biology have provided new approaches and tools for the industrialization of biology. In the next decade, advanced biocatalytic systems will increasingly be used for the production of chemicals that cannot be made by current processes and/or where the use of enzyme catalysts is more resource efficient with a much reduced environmental impact. We expect that in the future, manufacture of chemicals and materials will utilize both biocatalytic and chemical synthesis synergistically. The realization of such advanced biomanufacturing processes currently faces a number of major challenges. Ready‐to‐deploy portfolios of biocatalysts for design to production must be created from biological diverse sources and through protein engineering. Robust and efficient multi‐step enzymatic reaction cascades must be developed that can operate simultaneously in one‐pot. For this to happen, bio‐orthogonal strategies for spatial and temporal control of biocatalyst activities must be developed. Promising approaches and technologies are emerging that will eventually lead to the design of in vitro biocatalytic systems that mimic the metabolic pathways and networks of cellular systems which will be discussed in this roadmap.

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Biocatalytic portfolio of Basidiomycota

Abstract: Graphical abstract

Cited by 57 publications

References 80 publications

Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision

Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision

Analysis of basidiomycete pigments in situ by Raman spectroscopy

A roadmap for biocatalysis – functional and spatial orchestration of enzyme cascades

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