Biocatalytic living materials built by compartmentalized microorganisms in annealable granular hydrogels

Li, Yuan; Di, Zhengao; Yan, Xiaoqian; Wen, Hu; Cheng, Wei; Zhang, Jiang; Liu, Ji

doi:10.1016/j.cej.2022.136822

Cited by 17 publications

(15 citation statements)

References 43 publications

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“…The ink ingredients that are frequently used for extrusion-based 3D printing of living materials are soft, biocompatible hydrogels such as HA, silk fibroin, thermal-cross-linking collagen, gelatin, Pluronic F-127, ionic-cross-linking alginate, and photo-cross-linked poly (ethylene glycol) diacrylate (PEGDA) and gelatin methacrylamide (gelMA), which can be cured under mild conditions. The living components incorporated into the printed structure range from spores, bacteria, and yeast to algae and mammalian cells that exhibit unique biological functions (e.g., bioremediation, metabolite synthesis, biosensing, and bioenergy production). ,− In an early example, Schaffner et al developed a functional living ink (Flink) by combining HA and κ-carrageenan as natural viscoelastic gel components with fumed silica as a shear-thinning component (Figure a) . The ink was then blended with Pseudomonas putida or Acetobacter xylinum to print two types of “living materials” capable of degrading pollutants and producing medically relevant BC (Figure b) .…”

Section: Engineering Living Materials From a Materials Science Perspe...mentioning

confidence: 99%

Engineered Living Materials For Sustainability

Wang

Huang

et al. 2022

Chem. Rev.

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Recent advances in synthetic biology and materials science have given rise to a new form of materials, namely engineered living materials (ELMs), which are composed of living matter or cell communities embedded in self-regenerating matrices of their own or artificial scaffolds. Like natural materials such as bone, wood, and skin, ELMs, which possess the functional capabilities of living organisms, can grow, self-organize, and self-repair when needed. They also spontaneously perform programmed biological functions upon sensing external cues. Currently, ELMs show promise for green energy production, bioremediation, disease treatment, and fabricating advanced smart materials. This review first introduces the dynamic features of natural living systems and their potential for developing novel materials. We then summarize the recent research progress on living materials and emerging design strategies from both synthetic biology and materials science perspectives. Finally, we discuss the positive impacts of living materials on promoting sustainability and key future research directions.

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Section: Engineering Living Materials From a Materials Science Perspe...mentioning

confidence: 99%

Engineered Living Materials For Sustainability

Wang

Huang

et al. 2022

Chem. Rev.

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“…[4,5] A key component of this technology is the droplet-based microfluidic system, which utilizes passive microfluidic structures to quickly produce and control subnanometer-sized droplets in microchannel environments. [6,7] Droplets are formed continuously and robustly through the extrusion and shearing of two mutually immiscible phases in a microchannel. The volumes of these droplets are precisely controlled through variations of the flow rate ratios and channel dimensions.…”

Section: Introductionmentioning

confidence: 99%

Homogeneous, heterogeneous, and enzyme catalysis in microfluidics droplets

Mei

Lin

et al. 2023

Smart Molecules

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Microfluidics has received extensive attention due to its ability to rapidly prepare a large number of microdroplets with controlled sizes and defined morphologies. In addition to having large surface areas and controllable confinement environments, these prepared microdroplets can be used as analytical detection devices to screen and optimize various kinetic parameters. This review summarizes recent advances in the microfluidic control of droplet‐based catalytic reactions and discusses the role of these droplets in both homogeneous and heterogeneous catalyzes and in the catalysis of macromolecular biological enzymes in water‐in‐oil and oil‐in‐oil environments. Additionally, the existing problems and future development directions of droplets in catalysis are highlighted to promote the development of catalytic reactions in droplet media and provide guidance for the high‐throughput screening of catalysts and the directed evolution of biological enzymes.

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“…They allow for the diffusion of small molecules to maintain cell activity and growth while keeping cells in a stable network [19,20,21]. When a viscoelastic and shear-thinning hydrogel is utilized, it enables the bioprinting of cell-laden hydrogels, leading to precise control over the spatial distribution and concentration of microorganisms [22,23]. Although recent research has demonstrated the feasibility of cultivating multiple heterotrophs in hydrogels, the light-driven consortia, which have the potential to enable the reexamination of the global carbon cycle and other biogeochemical processes, have not been thoroughly investigated.…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic living fibers fabrication from algal-bacterial consortia with controlled spatial distribution

Sun¹,

Di²,

Zhang³

et al. 2023

Preprint

Self Cite

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Living materials that combine active cells and synthetic matrix materials have become a promising research field in recent years. While multicellular systems present exclusive benefits in developing living materials over single-cell systems, creating artificial multicellular systems can be challenging due to the difficulty in controlling the multicellular assemblies and the complexity of cell-to-cell interactions. Here, we propose a co-culture platform capable of isolating and controlling the spatial distribution of algal-bacterial consortia, which can be used to construct photosynthetic living fibers. Through coaxial extrusion-based 3D printing, hydrogel fibers containing bacteria or algae can be deposited into designated structures and further processed into materials with precise geometries. In addition, the photosynthetic living fibers demonstrate a significant synergistic catalytic effect resulting from the immobilization of both bacteria and algae, which effectively optimize sewage treatment for bioremediation purposes. The integration of microbial consortia and 3D printing gives functional living materials that have promising applications in biocatalysis, biosensing, and biomedicine. Our approach provides an optimized solution for constructing efficient multicellular systems and opens a new avenue for the development of advanced materials.

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Biocatalytic living materials built by compartmentalized microorganisms in annealable granular hydrogels

Cited by 17 publications

References 43 publications

Engineered Living Materials For Sustainability

Engineered Living Materials For Sustainability

Homogeneous, heterogeneous, and enzyme catalysis in microfluidics droplets

Photosynthetic living fibers fabrication from algal-bacterial consortia with controlled spatial distribution

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