Biocatalytic conversion of kraft pulp using cellulase complex of Penicillium verruculosum

Новожилов, Е. В.; Sinelnikov, Igor; Aksenov, Andrey; Чухчин, Д. Г.; Tyshkunova, Irina V.; Рожкова, А. М.; Осипов, Д. О.; Зоров, И. Н.; Sinitsyn, A. P.

doi:10.1134/s2070050416010098

Cited by 10 publications

(16 citation statements)

References 15 publications

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“…Kraft pulping also leads to hemicelluloses and some cellulose loss and decreases the degree of polymerization of cellulose [14]. The utilization of Kraft pulping as a pretreatment method for LCB has many advantages such as low sugar degradation, pulps free of inhibitors such as furfural and hydroxymethylfurfural (HMF), the recovery of chemicals used, and finally energy production in the recovery boiler [15,16].The enzymatic hydrolysis of Kraft pulp of sweet sorghum bagasse [17], pine, poplar, birch, beech, and wheat straw [16,18], hemp [18], eucalyptus [19], moso bamboo [20], spruce, and birch-aspen mixture [21] has been studied. Hydrolysates with sugar profiles adequate for fermentation have been obtained, proving the viability of Kraft pulping as a LCB pretreatment.…”

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confidence: 99%

“…The enzymatic hydrolysis of Kraft pulp of sweet sorghum bagasse [17], pine, poplar, birch, beech, and wheat straw [16,18], hemp [18], eucalyptus [19], moso bamboo [20], spruce, and birch-aspen mixture [21] has been studied. Hydrolysates with sugar profiles adequate for fermentation have been obtained, proving the viability of Kraft pulping as a LCB pretreatment.…”

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confidence: 99%

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Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

et al. 2020

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Second-generation bioethanol production's main bottleneck is the need for a costly and technically difficult pretreatment due to the recalcitrance of lignocellulosic biomass (LCB). Chemical pulping can be considered as a LCB pretreatment since it removes lignin and targets hemicelluloses to some extent. Chemical pulps could be used to produce ethanol. The present study aimed to investigate the batch ethanol production from unbleached Kraft pulp of Eucalyptus globulus by separate hydrolysis and fermentation (SHF). Enzymatic hydrolysis of the pulp resulted in a glucose yield of 96.1 ± 3.6% and a xylose yield of 94.0 ± 7.1%. In an Erlenmeyer flask, fermentation of the hydrolysate using Saccharomyces cerevisiae showed better results than Scheffersomyces stipitis. At both the Erlenmeyer flask and bioreactor scale, co-cultures of S. cerevisiae and S. stipitis did not show significant improvements in the fermentation performance. The best result was provided by S. cerevisiae alone in a bioreactor, which fermented the Kraft pulp hydrolysate with an ethanol yield of 0.433 g·g −1 and a volumetric ethanol productivity of 0.733 g·L −1 ·h −1 , and a maximum ethanol concentration of 19.24 g·L −1 was attained. Bioethanol production using the SHF of unbleached Kraft pulp of E. globulus provides a high yield and productivity.Energies 2020, 13, 744 2 of 15 sustainability, has a low and stable price, and practically does not demand extra land [6,7]. There are some facilities producing 2G bioethanol on a commercial scale. However, large-scale production still faces some technological barriers that must be overcome in order to achieve a cost-competitive production [8]. Due to the recalcitrance of LCB, a costly pretreatment step is required, which is the main technological bottleneck of 2G bioethanol production. The release of enzymatic and fermentation inhibitors during pretreatment is another limitation [9].Pulp and paper mills have the infrastructures and logistics to handle LCB, and chemical mills employ technology required for LCB fractionation and conversion [10]. Bioethanol has been produced from different feedstocks such as Kraft pulp, spent sulfite liquor, and pulp and paper sludge [11]. Chemical pulping processes can be considered as a LCB pretreatment since they promote delignification and target hemicelluloses to some degree [12]. Chemical pulping represents about 77% of the virgin pulps produced globally, and more than 95% of these chemical pulps are Kraft pulps [13]. These pulps are produced by Kraft pulping involving the reaction of white liquor, i.e., an alkaline aqueous solution of sodium hydroxide and sodium sulfide with a pH of 14, with lignin at high temperature (150-170 • C). This reaction promotes lignin breakdown and degradation with the release of phenolic fragments, removing almost 90% of the lignin from the wood. Kraft pulping also leads to hemicelluloses and some cellulose loss and decreases the degree of polymerization of cellulose [14]. The utilization of Kraft pulping as a pretreatment method for LCB has ...

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confidence: 99%

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Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

et al. 2020

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“…The present work is a continuation of the previous works [9,11,30], which aimed at obtaining hydrolysates with high content of cellulosic sugars after kraft method of wood raw materials pretreatment by means of conversion with enzyme complexes based on the producer strains P. verruculosum. This study deals with modified kraft pulp produced by such biocatalysis.…”

Section: Introductionmentioning

confidence: 87%

“…Among these, for example, are acid treatment, alkali cooking and kraft pulping. Kraft pulping is a cost-efficient and effective method of preparing wood raw materials for the production of pulp with good reactivity for enzymatic conversion [11][12][13][14][15][16]. Nowadays, the current high level of technology allows the integration of biorefineries into existing pulp and paper industrial enterprises [5,6,[17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Production of Biomodified Bleached Kraft Pulp by Catalytic Conversion Using Penicillium verruculosum Enzymes: Composition, Properties, Structure and Application

Shevchenko¹,

Tyshkunova²,

Малков³

et al. 2022

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The global development of bioeconomy is impossible without technologies for comprehensive processing of plant renewable resources. The use of proven pretreatment technologies raises the possibility of industrial implementation of enzymatic conversion of polysaccharides from lignocellulose considering the process complexity. For instance, a well-tuned kraft pulping produces a substrate easily degraded by cellulases and hemicelulases. Enzymatic hydrolysis of bleached hardwood kraft pulp was carried out using an enzyme complex of endoglucanases, cellobiohydrolases, b-glucosidases and xylanases produced by recombinant strains of Penicillium verruculosum at a 10 FPU/g mixture rate and a 10 % substrate concentration. As a result of biocatalysis the following products are obtained: sugar solution, mainly glucose, xylobiose, xylose, as well as other minor reducing sugars; modified complex based on cellulose and xylan. The composition of biomodified kraft pulp was determined involving the use of HPLC. The method for determining the degree of crystallinity on an X-ray diffractometer was used to characterize the properties. The article shows the possibility of producing modified cellulose cryogels by amorphization with concentrated 85 % H3PO4 followed by precipitation with water and supercritical drying. Analysis of the enzymatic hydrolysate composition reveals the predominance of glucose (55...67 %) in reducing sugars with a maximum content of up to 6 % in the solution after 72 hours. The properties and structure of modified kraft pulp were shown to change during biocatalysis, in particular, the degree of crystallinity increased by 5 % after 3 hours of enzymatic hydrolysis. We obtained cryogels based on the initial and biomodified kraft pulp with conversion rates of 35, 50 and 70 %. The properties of these cryogels are not inferior to those based on industrial microcrystalline cellulose, as confirmed by the specific surface area, degree of swelling, porosity and SEM images. Thus, kraft pulp enzymatic hydrolysis offers prospects not only for producing sugar-rich hydrolysates for microbiological synthesis, but also for producing cellulose powders and cryogels with specified properties.

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“…Different studies already proved the viability of Kraft pulping as a LCB pretreatment by obtaining hydrolysates with sugar profiles suitable for fermentation through enzymatic hydrolysis of Kraft pulp of different origins, including sweet sorghum bagasse [97], pine, poplar, birch, beech, and wheat straw [96,98], hemp [98], eucalyptus [99], moso bamboo [100], spruce, and birch-aspen mixture [101]. Also, several studies focused on the fermentation configuration to produce ethanol from Kraft pulp.…”

Section: Bioethanol Productionmentioning

confidence: 99%

Second Generation Bioethanol Production: On the Use of Pulp and Paper Industry Wastes as Feedstock

2018

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Due to the health and environment impacts of fossil fuels utilization, biofuels have been investigated as a potential alternative renewable source of energy. Bioethanol is currently the most produced biofuel, mainly of first generation, resulting in food-fuel competition. Second generation bioethanol is produced from lignocellulosic biomass, but a costly and difficult pretreatment is required. The pulp and paper industry has the biggest income of biomass for non-food-chain production, and, simultaneously generates a high amount of residues. According to the circular economy model, these residues, rich in monosaccharides, or even in polysaccharides besides lignin, can be utilized as a proper feedstock for second generation bioethanol production. Biorefineries can be integrated in the existing pulp and paper industrial plants by exploiting the high level of technology and also the infrastructures and logistics that are required to fractionate and handle woody biomass. This would contribute to the diversification of products and the increase of profitability of pulp and paper industry with additional environmental benefits. This work reviews the literature supporting the feasibility of producing ethanol from Kraft pulp, spent sulfite liquor, and pulp and paper sludge, presenting and discussing the practical attempt of biorefineries implementation in pulp and paper mills for bioethanol production.

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Biocatalytic conversion of kraft pulp using cellulase complex of Penicillium verruculosum

Cited by 10 publications

References 15 publications

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

Production of Biomodified Bleached Kraft Pulp by Catalytic Conversion Using Penicillium verruculosum Enzymes: Composition, Properties, Structure and Application

Second Generation Bioethanol Production: On the Use of Pulp and Paper Industry Wastes as Feedstock

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