Biocatalytic carbon nanotube paper: a ‘one-pot’ route for fabrication of enzyme-immobilized membranes for organophosphate bioremediation

Mechrez, Guy; Krepker, Maksym A.; Harel, Yifat; Lellouche, Jean‐Paul; Segal, Ester

doi:10.1039/c3tb21439g

Cited by 43 publications

(15 citation statements)

References 72 publications

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“…[20][21][22] They have been proven as an efficient enzyme immobilization support. Mechrez et al 24 prepared a carboxylated CNT coating on a microltration membrane support, which was further applied for organophosphate hydrolase (OPH) immobilization. Lannoy et al 23 prepared a highly stable CNT coating layer (less than 5 mm) on a microltration membrane via cross-linking, and the coating layer exhibited good hydrophilicity and conductivity.…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic Janus membranes for CO₂ removal utilizing carbonic anhydrase

Hou,

Ji,

Dong

et al. 2015

J. Mater. Chem. A

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A novel hydrophilic-superhydrophobic biocatalytic membrane was developed for CO 2 capture with a gasliquid membrane contactor. This "Janus" membrane contains a layer of hydrophilic carbon nanotubes (CNTs) coated on a fluorosilane treated superhydrophobic membrane support. Carbonic anhydrase (CA) was then immobilized on the hydrophilic CNT side, which was located at the CO 2 -solvent interface within a gas-liquid membrane contactor, whilst the superhydrophobic porous side of the membrane was oriented towards the gas phase. This "Janus" configuration ensured that the immobilized CA remained hydrated, and minimized the CO 2 diffusion length in the solvent. The CNT coating layer showed good integrity and adhesion to the membrane, and the effect of superhydrophobic treatment on the porous structure of the membrane was negligible. The p-NPA assay results revealed that up to 30% CA activity was retained after immobilization. Further, the CO 2 hydration test confirmed that the immobilized CA possessed significantly improved catalytic efficiency when compared with the equal amount of free CA. Effective regeneration of the enzyme coating was demonstrated over five cycles. The novel "Janus" membrane developed in this study exhibits great potential to be used as an immobilization support for a wide variety of enzymes not only CA for CO 2 capture but also other types of enzymes in different applications.

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Section: Introductionmentioning

confidence: 99%

Biocatalytic Janus membranes for CO₂ removal utilizing carbonic anhydrase

Hou,

Ji,

Dong

et al. 2015

J. Mater. Chem. A

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“…Organophosphates are among the most toxic substances synthesized to date, and are used as pesticides and nerve agents [ 93 ]. Recently, a new ‘one-pot’ methodology was developed for the rapid and straightforward fabrication of an enzymatically active MWNT BP to be used for organophosphate bioremediation [ 94 ]. This new type of BP was prepared from carboxylated MWNTs (MWNTs–COOH), which were ultrasonically dispersed in an aqueous solution containing Triton X-100.…”

Section: Filtration Applicationsmentioning

confidence: 99%

Carbon Nanotube Membranes: Synthesis, Properties, and Future Filtration Applications

2017

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Over the course of the past decade, there has been growing interest in the development of different types of membranes composed of carbon nanotubes (CNTs), including buckypapers and composite materials, for an ever-widening range of filtration applications. This article provides an overview of how different types of CNT membranes are prepared and the results obtained from investigations into their suitability for different applications. The latter involve the removal of small particles from air samples, the filtration of aqueous solutions containing organic compounds and/or bacteria, and the separation of individual liquids present in mixtures. A growing number of reports have demonstrated that the incorporation of CNTs into composite membranes confers an improved resistance to fouling caused by biomacromolecules and bacteria. These results are discussed, along with evidence that demonstrates it is possible to further reduce fouling by taking advantage of the inherent conductivity of composite membranes containing CNTs, as well as by using different types of electrochemical stimuli.

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“…The carboxylic acid groups were introduced by oxidation of CNTs and activation using carbodiimide [93,94]. Lipase [95,96], organophosphorous hydrolase [97,98] and other enzymes have been successfully immobilized onto CNTs. Alternatively, enzyme molecules are covalently attached to linking molecules on the CNT surface via hydrophobic or p-p interactions.…”

Section: Carbon Nanocarriersmentioning

confidence: 99%

Nanobiocatalyst advancements and bioprocessing applications

Misson

Zhang

Jin

2015

J. R. Soc. Interface.

210

108

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The nanobiocatalyst (NBC) is an emerging innovation that synergistically integrates advanced nanotechnology with biotechnology and promises exciting advantages for improving enzyme activity, stability, capability and engineering performances in bioprocessing applications. NBCs are fabricated by immobilizing enzymes with functional nanomaterials as enzyme carriers or containers. In this paper, we review the recent developments of novel nanocarriers/nanocontainers with advanced hierarchical porous structures for retaining enzymes, such as nanofibres (NFs), mesoporous nanocarriers and nanocages. Strategies for immobilizing enzymes onto nanocarriers made from polymers, silicas, carbons and metals by physical adsorption, covalent binding, cross-linking or specific ligand spacers are discussed. The resulting NBCs are critically evaluated in terms of their bioprocessing performances. Excellent performances are demonstrated through enhanced NBC catalytic activity and stability due to conformational changes upon immobilization and localized nanoenvironments, and NBC reutilization by assembling magnetic nanoparticles into NBCs to defray the high operational costs associated with enzyme production and nanocarrier synthesis. We also highlight several challenges associated with the NBC-driven bioprocess applications, including the maturation of large-scale nanocarrier synthesis, design and development of bioreactors to accommodate NBCs, and long-term operations of NBCs. We suggest these challenges are to be addressed through joint collaboration of chemists, engineers and material scientists. Finally, we have demonstrated the great potential of NBCs in manufacturing bioprocesses in the near future through successful laboratory trials of NBCs in carbohydrate hydrolysis, biofuel production and biotransformation.

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Biocatalytic carbon nanotube paper: a ‘one-pot’ route for fabrication of enzyme-immobilized membranes for organophosphate bioremediation

Abstract: A generic methodology for a rapid and direct fabrication of enzymatically-active carbon nanotubes (CNTs) paper for organophosphates bioremediation is presented. The enzyme organophosphate hydrolase is immobilized onto CNTs simultaneously to membrane formation process.

Cited by 43 publications

References 72 publications

Biocatalytic Janus membranes for CO₂ removal utilizing carbonic anhydrase

Biocatalytic Janus membranes for CO₂ removal utilizing carbonic anhydrase

Carbon Nanotube Membranes: Synthesis, Properties, and Future Filtration Applications

Nanobiocatalyst advancements and bioprocessing applications

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Biocatalytic carbon nanotube paper: a ‘one-pot’ route for fabrication of enzyme-immobilized membranes for organophosphate bioremediation

Abstract: A generic methodology for a rapid and direct fabrication of enzymatically-active carbon nanotubes (CNTs) paper for organophosphates bioremediation is presented. The enzyme organophosphate hydrolase is immobilized onto CNTs simultaneously to membrane formation process.

Cited by 43 publications

References 72 publications

Biocatalytic Janus membranes for CO2 removal utilizing carbonic anhydrase

Biocatalytic Janus membranes for CO2 removal utilizing carbonic anhydrase

Carbon Nanotube Membranes: Synthesis, Properties, and Future Filtration Applications

Nanobiocatalyst advancements and bioprocessing applications

Contact Info

Product

Resources

About

Biocatalytic Janus membranes for CO₂ removal utilizing carbonic anhydrase

Biocatalytic Janus membranes for CO₂ removal utilizing carbonic anhydrase