Biocatalysts entrapped in silica gels

Reactive Separations for In Situ Product Removal of Enzymatic Reactions: A Review

“…Although, similarly to the case of encapsulation, activity of enzymes immobilized by entrapment can be lowered by diffusional limitations , the mild conditions of entrapment can lead to apparent activities exceeding those of enzymes immobilized by covalent bonding . One of the key advantages of biocatalyst entrapment is the broad spectrum of shapes that the structures can take, including coatings , paints , granulates , cylinders , and more. Cross‐linking, which – unlike the aforementioned methods – does not depend on binding the enzyme to a carrier but on binding the enzyme molecules directly to each other. Cross‐linking can be achieved by binding NH 2 functional groups of amino acids to bifunctional linker such as glutaraldehyde .…”