Biocatalysis in the Fine Chemical and Pharmaceutical Industries

Sutton, Peter W.; Adams, Joseph P.; Archer, Ian; Auriol, Daniel; Avi, Manuela; Branneby, Cecilia; Collis, Andrew J.; Dumas, Bruno; Eckrich, Thomas M.; Fotheringham, Ian; Halle, Rob ter; Hanlon, Steven P.; Hansen, Marvin M.; Holt-Tiffin, Karen E.; Howard, Roger M.; Huisman, Gjalt W.; Iding, Hans; Kiewel, Kurt; Kittelmann, Matthias; Kupfer, Ernst; Laumen, Kurt; Lefèvre, Fabrice; Luetz, Stephan; Mangan, David; Martin, Van A.; Meyer, Hans‐Peter; Moody, Thomas S.; Osorio‐Lozada, Antonio; Robins, Karen; Šnajdrová, Radka; Truppo, Matthew D.; Wells, Andrew S.; Wirz, Beat; Wong, John W.

doi:10.1002/9781119943426.ch1

Cited by 8 publications

(6 citation statements)

References 101 publications

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“…In search of novel techniques towards a sustainable and biobased economy, biocatalysis contributes to various areas from the production of bulk chemicals from renewable resources to highly selective syntheses of fine chemicals or pharma products [1][2][3][4]. In all these applications, whole cell biocatalysts supply some unique advantages over the use of crude, purified, or immobilized enzyme preparations.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Wachtmeister

Rother

2016

Current Opinion in Biotechnology

269

227

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Section: Introductionmentioning

confidence: 99%

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Wachtmeister

Rother

2016

Current Opinion in Biotechnology

269

227

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“…Enzymes are widely used as biocatalysts in pharmaceuticals, fine chemicals, flavors and fragrances, vitamins, and the food and textile industries . The use of biocatalysts has become popular in the last few decades due to its environmental friendliness, safety, and excellent chemo-, regio-, and stereoselectivity .…”

Section: Introductionmentioning

confidence: 99%

Using Spinchem Rotating Bed Reactor Technology for Immobilized Enzymatic Reactions: A Case Study

Pithani

Karlsson

Emtenäs

et al. 2019

Org. Process Res. Dev.

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The practically and financially viable use of immobilized enzymes in the production of pharmaceutical and bulk chemicals requires highly catalytically active enzymes and efficient mass transfer of substrate to the active site. The substantial cost of immobilized enzymes further mandates extended reuse of the enzymes to keep operating expenditures down. The extent of reuse of immobilized enzymes depends on parameters such as enzyme long-term stability under process conditions, carrier integrity and attrition due to mechanical stress, and ease of workup and recovery on scale. Herein, we report on the use of a SpinChem rotating bed reactor (RBR) for the Novozyme 435 mediated kinetic resolution of (cis)-isopropyl 3aminocyclohexanecarboxylate as an alternative to the traditional reaction using free-flowing immobilized lipases in an ordinary batch reactor. Reusability was studied in addition to other parameters such as type of immobilized enzyme, loading, rate of agitation, temperature, and scalability. By using SpiChem RBR technology, we found that recycling of the immobilized enzyme was easy with preserved enantioselectivity and catalytic activity. The final optimized process was successfully demonstrated on a 1 kg scale with 39% isolated yield and 98.8% enantiomeric purity.

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“…The biggest challenge for an efficient process was the immobilization of CalB. The immobilization resin EXE120 retained 94% activity after 48 h, whereas the classical Novozym 435 preparation was rapidly deactivated (6% retained activity) . The process was scaled up at 100 kg with high yields (95%) and an e.e.…”

Section: Functional Groups Formed In Biocatalytic Reactions For Api S...mentioning

confidence: 99%

“…Furthermore, only examples that were applied on ideally at least several gram scale or where information on industrial application is available are included. Complementary reviews are suggested. ,,− …”

Section: Introductionmentioning

confidence: 99%

Shortening Synthetic Routes to Small Molecule Active Pharmaceutical Ingredients Employing Biocatalytic Methods

et al. 2021

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Biocatalysis, using enzymes for organic synthesis, has emerged as powerful tool for the synthesis of active pharmaceutical ingredients (APIs). The first industrial biocatalytic processes launched in the first half of the last century exploited whole-cell microorganisms where the specific enzyme at work was not known. In the meantime, novel molecular biology methods, such as efficient gene sequencing and synthesis, triggered breakthroughs in directed evolution for the rapid development of process-stable enzymes with broad substrate scope and good selectivities tailored for specific substrates. To date, enzymes are employed to enable shorter, more efficient, and more sustainable alternative routes toward (established) small molecule APIs, and are additionally used to perform standard reactions in API synthesis more efficiently. Herein, large-scale synthetic routes containing biocatalytic key steps toward >130 APIs of approved drugs and drug candidates are compared with the corresponding chemical protocols (if available) regarding the steps, reaction conditions, and scale. The review is structured according to the functional group formed in the reaction.

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Biocatalysis in the Fine Chemical and Pharmaceutical Industries

Cited by 8 publications

References 101 publications

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Using Spinchem Rotating Bed Reactor Technology for Immobilized Enzymatic Reactions: A Case Study

Shortening Synthetic Routes to Small Molecule Active Pharmaceutical Ingredients Employing Biocatalytic Methods

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