Biocatalysis in ionic liquids: batchwise and continuous flow processes using supercritical carbon dioxide as the mobile phase

Abstract

A new and environmentally benign protocol for enzymatic reactions in ionic liquids is described using supercritical CO2 as the mobile phase; the products are obtained in solvent-free form and the enzyme/ionic liquid mixture can be recycled in batchwise or continuous flow operations.

Homogeneous Catalysis and Catalyst Recovery Using Supercritical Carbon Dioxide and Ionic Liquids

“…2a) and Leitner (Fig. 2b) Reetz et al 33 had been simultaneously studying these reactions and obtained similar results.…”

Ionic liquids in biotechnology: applications and perspectives for biotransformations

“…The kinetic resolution of secondary alcohols in the system IL/scCO 2 was target of investigations of Reetz et al (2002Reetz et al ( , 2003 ] as reactive phase for the continuous kinetic resolution of rac-1-phenylethanol with CALB. The enzyme was imposed to celite, suspended in IL, and used in 4 h reaction-extraction cycles followed by 20 h of storage at room temperature.…”

Application of Ionic Liquids in Biocatalysis

“…The efficiency of the system was depended on both the mass-transfer phenomena between ILs and SC CO 2 immiscible phases and the specificity of the enzyme toward the catalyzed reaction. At lipase-catalyzed kinetic resolution of rac-1-phenylethanol with vinyl acetate in 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)amide ([bmim][bta]) using SC CO 2 in a batch-wise process the enzyme fully retained its activity and the enantiomeric discrimination remained uniformly high (Reetz et al, 2002). Garcia et al (Garcia et al, 2004) (Miyawaki & Tatsuno, 2008) studied lipase-catalyzed butanolysis of triolein in an IL methyltrioctylammonium trifluoroacetate ([mtoa][tfa]) and afterwards the product, butyloleate, was selectively extracted from the reaction mixture using SC CO 2 at 35 °C and 8.6 MPa.…”