Biocatalysis for Drug Discovery and Development

Li, Tiehai; Zhang, Mingzi M.; Ang, Ee Lui; Zhao, Huimin

doi:10.1002/9781118828083.ch16

Cited by 3 publications

(1 citation statement)

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“…Biocatalysis is an alternative way of synthesis of phenylethyl alcohol derivatives. Reported protocols employing fungal mycelia have proved their utility and also are simple, cost-saving and environmentally friendly [17][18][19][20]. Employing free or immobilized forms of biocatalyst or resting spores have allowed manifesting of different enzymatic activities by triggering different metabolic pathways, which have resulted in the synthesis of different phenolic derivatives [17,21,22].…”

Section: Introductionmentioning

confidence: 99%

Half-Preparative Scale Synthesis of (S)-1-Phenylethane-1,2-Diol as a Result of 2-Phenylethanol Hydroxylation with Aspergillus niger (IAFB 2301) Assistance

et al. 2020

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Aspergillus niger (IAFB 2301) was employed for bioconversions of 2-phenylethanol as an immobilized or free mycelium and also as a spore suspension. Experiments were conducted on laboratory and half-preparative scale (bioreactor New Brunswick Scientific, BioFlo Model C32). Thus, A. niger applied as free mycelium, depending on the outcome, supported formation of the mixture of 4-hydroxyphenylacetic acid and hydroxytyrosol (final concentration of 13.8 mg/L and 3.7% efficiency) or 4-hydroxyphenylacetic acid, as single product (final concentration of 140 mg/L and 18% efficiency). In case of scaling experiments conducted with flow and batch reactors, accordingly, the following results were achieved: 1. mixture of antioxidants 4-hydroxyphenylacetic acid and hydroxytyrosol formed with final concentration of 76 mg/L and 10% efficiency (simplified flow system and immobilized mycelium); 2. (S)-1-phenylethane-1,2-diol synthesized with a final concentration of 447 mg/L and 65% (1.3 L batch reactor).

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