Biocatalysis

Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

“…2 For the realization of high enantiopurities, enzymes have established themselves as key catalysts and provide the most atom efficient route towards chiral building blocks, as recognized by many Presiden-tial Green Chemistry awards. 3,4 A prime example of highly selective biocatalysts are hydroxynitrile lyases (HNLs). This group of versatile enzymes catalyzes the enantioselective addition of nucleophilic cyanide to prochiral carbonyl compounds, realizing the formation of industrially important chiral cyanohydrins.…”

Kinetics of epoxidation by a Musa paradisiaca chloroperoxidase

“…Extensive studies on carbon-carbon double bonds have been reported in the literature giving varying degrees of enantiomeric excesses of the epoxides products formed using chemical and biological catalysts. [5][6][7][8][9][10][11][12][13][14][15]19,28,29 However, all the reported studies either involve expensive metal catalysts or purified enzymes. The present communication reports epoxidation using concentrated juice of M. paradisiaca (banana) stem as the crude enzyme.…”

Enzymatic conversion of CO2 to CH3OH via reverse dehydrogenase cascade biocatalysis: Quantitative comparison of efficiencies of immobilized enzyme systems

“…Unfortunately, only limited work has apparently been done on optimizing the systems by balancing the kinetics of the main reaction with the kinetics of the regeneration reaction or on transferring knowledge from one recycling system to another. Some of the common regenerating enzymes and the reactions employed in enzyme-cofactor regeneration systems, beyond FDH and ADH, have been listed in Table 5 [57].…”