Biobutanol – An impending biofuel for future: A review on upstream and downstream processing tecniques

Bharathiraja, B.; Jayamuthunagai, J.; Sudharsanaa, T.; Bharghavi, A.; Praveenkumar, R.; Chakravarthy, M.; Yuvaraj, D.

doi:10.1016/j.rser.2016.10.017

Cited by 183 publications

(74 citation statements)

References 209 publications

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“…The main components of lignocellulose are cellulose (35–50%), hemicellulose (20–35%), and lignin (10–25%), with small percentages of pectin depending on species, growth, and tissue . Cellulose and hemicelluloses (xyloglucan, xylan, mannans, glucomannans, and β‐(1,3‐1,4)‐glucans) are made of equatorial β‐(1,4)‐linked backbone structure while pectins (galactans, arabinans, and arabinogalactans) contain axial and equatorial backbone structures.…”

Section: Introductionmentioning

confidence: 99%

“…Cellulose and hemicelluloses (xyloglucan, xylan, mannans, glucomannans, and β‐(1,3‐1,4)‐glucans) are made of equatorial β‐(1,4)‐linked backbone structure while pectins (galactans, arabinans, and arabinogalactans) contain axial and equatorial backbone structures. In second‐generation biorefineries, C6 and C5 sugars are produced by chemico‐physical pretreatment (steam explosion and pretreatment with acid or alkali) of the lignocellulose biomass and then fermented to ethanol or bioplastic precursors . Because of the recalcitrant nature of plant biomass, the complete hydrolysis of the lignocellulose is one of the main bottlenecks to fully realize this industrial process.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of hyperstable carbohydrate‐active enzymes through metagenomics of extreme environments

et al. 2019

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The enzymes from hyperthermophilic microorganisms populating volcanic sites represent interesting cases of protein adaptation and biotransformations under conditions where conventional enzymes quickly denature. The difficulties in cultivating extremophiles severely limit access to this class of biocatalysts. To circumvent this problem, we embarked on the exploration of the biodiversity of the solfatara Pisciarelli, Agnano (Naples, Italy), to discover hyperthermophilic carbohydrate‐active enzymes (CAZymes) and to characterize the entire set of such enzymes in this environment (CAZome). Here, we report the results of the metagenomic analysis of two mud/water pools that greatly differ in both temperature and pH (T = 85 °C and pH 5.5; T = 92 °C and pH 1.5, for Pool1 and Pool2, respectively). DNA deep sequencing and following in silico analysis led to 14 934 and 17 652 complete ORFs in Pool1 and Pool2, respectively. They exclusively belonged to archaeal cells and viruses with great genera variance within the phylum Crenarchaeota, which reflected the difference in temperature and pH of the two Pools. Surprisingly, 30% and 62% of all of the reads obtained from Pool1 and 2, respectively, had no match in nucleotide databanks. Genes associated with carbohydrate metabolism were 15% and 16% of the total in the two Pools, with 278 and 308 putative CAZymes in Pool1 and 2, corresponding to ~ 2.0% of all ORFs. Biochemical characterization of two CAZymes of a previously unknown archaeon revealed a novel subfamily GH5_19 β‐mannanase/β‐1,3‐glucanase whose hemicellulose specificity correlates with the vegetation surrounding the sampling site, and a novel NAD+‐dependent GH109 with a previously unreported β‐N‐acetylglucosaminide/β‐glucoside specificity. Databases The sequencing reads are available in the NCBI Sequence Read Archive (SRA) database under the accession numbers SRR7545549 (Pool1) and SRR7545550 (Pool2). The sequences of GH5_Pool2 and GH109_Pool2 are available in GenBank database under the accession numbers MK869723 and MK86972, respectively. The environmental data relative to Pool1 and Pool2 (NCBI BioProject PRJNA481947) are available in the Biosamples database under the accession numbers SAMN09692669 (Pool1) and SAMN09692670 (Pool2).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discovery of hyperstable carbohydrate‐active enzymes through metagenomics of extreme environments

et al. 2019

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“…1a) [5]. The global market for butanol was estimated to be 2.8 million tons annually, which is approximately 5 billion USD [6]. Owing to the advantages of butanol over ethanol, there is an increase in the demand for butanol ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review

Nawab

Wang

et al. 2020

Microb Cell Fact

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Background: Owing to the increase in energy consumption, fossil fuel resources are gradually depleting which has led to the growing environmental concerns; therefore, scientists are being urged to produce sustainable and ecofriendly fuels. Thus, there is a growing interest in the generation of biofuels from renewable energy resources using microbial fermentation. Main text: Butanol is a promising biofuel that can substitute for gasoline; unfortunately, natural microorganisms pose challenges for the economical production of 1-butanol at an industrial scale. The availability of genetic and molecular tools to engineer existing native pathways or create synthetic pathways have made non-native hosts a good choice for the production of 1-butanol from renewable resources. Non-native hosts have several distinct advantages, including using of cost-efficient feedstock, solvent tolerant and reduction of contamination risk. Therefore, engineering non-native hosts to produce biofuels is a promising approach towards achieving sustainability. This paper reviews the currently employed strategies and synthetic biology approaches used to produce 1-butanol in non-native hosts over the past few years. In addition, current challenges faced in using non-native hosts and the possible solutions that can help improve 1-butanol production are also discussed. Conclusion: Non-native organisms have the potential to realize commercial production of 1-butanol from renewable resources. Future research should focus on substrate utilization, cofactor imbalance, and promoter selection to boost 1-butanol production in non-native hosts. Moreover, the application of robust genetic engineering approaches is required for metabolic engineering of microorganisms to make them industrially feasible for 1-butanol production.

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“…In spite of all the above-mentioned advantages, biobutanol production has remained uneconomical (Bharathiraja et al, 2017). The cost associated with the separation/purification step is regarded as the main obstacle of a successful biobutanol production (García et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Due to the high boiling point of 1-butanol, i.e., 118 o C, water will evaporate more significantly (Oudshoorn et al, 2009). The relative evaporation is disadvantageous for butanol production given the fact that the maximum concentration of butanol in the aqueous phase is only ~ 70 g/L ( Barton and Haulait-Pirson, 1984;Sakashita et al, 2010;Bharathiraja et al, 2017) or 6 wt.% (Cheng and Park, 2017). Furthermore, the biological process cannot tolerate high butanol concentrations.…”

Section: Introductionmentioning

confidence: 99%

Selective evaporation of a butanol/water droplet by microwave irradiation, a step toward economizing biobutanol production

et al. 2020

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Selective evaporation of a butanol/water droplet by microwave irradiation, a strategy to economize biobutanol production.Journal homepage: www.biofueljournal.com HIGHLIGHTS ➢Surface and evaporation of alcohol/water was studied in-situ in response to microwave irradiation. ➢Butanol content in liquid phase was reduced by approx. 90%. ➢Similar trend was observed with pentanol/water droplet. ➢The method could be applied to improve efficiency of fermentative butanol separation. GRAPHICAL ABSTRACT ARTICLE INFO ABSTRACT Article history:The separation step is a constraint in biobutanol production, due to high energy consumption of the current techniques. This study explores a new separation method via applying microwave irradiation. Butanol/water droplet was monitored during exposure to microwave irradiation at different power rates. The surface tension, droplet volume, and temperature were scrutinized during and after exposure to microwave irradiation. The data obtained indicated that the microwave-induced evaporation rates of alcohols were much higher than that of water. Consequently, the vaporized phase contained a much higher alcohol content than the liquid phase. In particular, butanol concentration in the aqueous phase could be reduced to ~ 0.1 wt.%, which was more effective than the theoretical limit via the boiling process. The method is an attractive option to complement the fermentation process, which produces a low butanol concentration solution. This development could potentially lead to a more efficient pathway for biobutanol production. Selective evaporation of a butanol/water droplet by microwave irradiation, a strategy to economize biobutanol production.

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Biobutanol – An impending biofuel for future: A review on upstream and downstream processing tecniques

Cited by 183 publications

References 209 publications

Discovery of hyperstable carbohydrate‐active enzymes through metagenomics of extreme environments

Discovery of hyperstable carbohydrate‐active enzymes through metagenomics of extreme environments

Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review

Selective evaporation of a butanol/water droplet by microwave irradiation, a step toward economizing biobutanol production

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