Bioburden vs. Antibiogram of Diabetic Foot Infection

Sekkizhar, Janifer Jasmine Geethalakshmi

doi:10.4172/2329-910x.1000121

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…(4) presentation A 15 to 30 fold increase risk has been observed in the diabetics when compared to non-diabetic patients. Globally, a lower limb amputation (complete or partial) takes ( 5 ) place every 30 seconds. The study was aimed at ascer taining the etiology, var ious clinical profile, complications and outcome of the different treatment modalities used in its treatment.…”

Section: Introductionmentioning

confidence: 99%

Management of Diabetic Foot Ulcers and Related Complications: A Prospective Observational Study

Sharma¹,

Sharma²,

Gupta³

2023

PIJR

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Introduction Foot ulceration and its complications are one of the major complications attributing to the hospital admission, with overall prevalence of about 4 -10% and even higher in geriatric population (5-10%).With the lifetime risk of 15%,it contributes as a major factor of morbidity and not infrequently leading to severe adverse outcomes, such as amputation and life threatening sepsis.The study was aimed at analyzing the prevalence of diabetic foot infection,its complications and outcomes of different treatment modalities available in a tertiary care center.Material and Methods A prospective observational study of 12 month duration was conducted among 100 patients having a history of diabetes and diagnosed with diabetic foot ulcer undergoing treatment in the surgery department of a tertiary care center in Jammu.The period of study was Feb 2022 to Feb 2023.Prior to the study an informed written consent and approval of the ethics committee was obtained. Results The incidence of diabetic foot ulcers peaks at the 5th decade of life with the mean age in our study being 53.17±10.2 years.A predominance in the male population (m:f ratio=2.57:1) and higher risk in the lower socio-economic strata was also noticed. The most common presenting complaint being ulcer (96%) and discharge (67%).Culture positivity was seen in 80% of the patients with Staph.Aureus being the most common causative organism. The main stray of treatment involved debridement and amputation (31%) followed by Split thickness skin grafting (30%). No adverse events were noted during the course of study. Conclusion Being a menace in developing countries,diabetes mellitus,a disease with life threatening and physically incapacitating consequences which not only increases the patient morbidity but also substantially increases the economic burden. Provision of precise counseling and a multidisciplinary line of action can lessen the complication and amputation rate, and is therefore recommended, along with the incorporation of diabetic foot management guidelines into the routine practice and teaching

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Section: Introductionmentioning

confidence: 99%

Management of Diabetic Foot Ulcers and Related Complications: A Prospective Observational Study

Sharma¹,

Sharma²,

Gupta³

2023

PIJR

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Isolation and Genetic Analysis of Multidrug Resistant Bacteria from Diabetic Foot Ulcers

Shahi

Kumar

2016

Front. Microbiol.

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Severe diabetic foot ulcers (DFUs) patients visiting Sir Sunderlal Hospital, Banaras Hindu University, Varanasi, were selected for this study. Bacteria were isolated from swab and deep tissue of 42 patients, for examining their prevalence and antibiotic sensitivity. DFUs of majority of the patients were found infected with Enterococcus spp. (47.61%), Escherichia coli (35.71%), Staphylococcus spp. (33.33%), Alcaligenes spp. (30.95%), Pseudomonas spp. (30.95%), and Stenotrophomonas spp. (30.95%). Antibiotic susceptibility assay of 142 bacteria with 16 antibiotics belonging to eight classes showed the presence of 38 (26.76%) isolates with multidrug resistance (MDR) phenotypes. MDR character appeared to be governed by integrons as class 1 integrons were detected in 26 (68.42%) isolates. Altogether six different arrays of genes (aadA1, aadB, aadAV, dhfrV, dhfrXII, and dhfrXVII) were found within class 1 integron. Gene cassette dhfrAXVII-aadAV (1.6 kb) was present in 12 (3 Gram positive and 9 Gram negative) isolates and was conserved across all the isolates as evident from RFLP analysis. In addition to the presence of class 1 integron, six β-lactamase resistance encoding genes namely blaTEM, blaSHV, blaOXA, blaCTX−M−gp1, blaCTX−M−gp2, and blaCTX−M−gp9 and two methicillin resistance genes namely mecA and femA and vancomycin resistance encoding genes (vanA and vanB) were identified in different isolates. Majority of the MDR isolates were positive for blaTEM (89.47%), blaOXA (52.63%), and blaCTX−M−gp1 (34.21%). To our knowledge, this is the first report of molecular characterization of antibiotic resistance in bacteria isolated from DFUs from North India. In conclusion, findings of this study suggest that class-1 integrons and β-lactamase genes contributed to the MDR in above bacteria.

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Bioburden vs. Antibiogram of Diabetic Foot Infection

Cited by 2 publications

References 23 publications

Management of Diabetic Foot Ulcers and Related Complications: A Prospective Observational Study

Management of Diabetic Foot Ulcers and Related Complications: A Prospective Observational Study

Isolation and Genetic Analysis of Multidrug Resistant Bacteria from Diabetic Foot Ulcers

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