Abstract:Today, application of in vitro culture by means of slow growth storage of shoot cultures and cryopreservation of organs, tissues and cells in liquid nitrogen presents a remarkable strategic tool to support medium-and long-term conservation of plant genetic resources. Over the last 30 years, considerable progresses have been made in the development of both methods that are currently considered as ex situ conservation strategies, complementary to traditional seed banks and in-field clonal collections. Efficient … Show more
“…Cryopreservation is an important tool for storing germplasm, developed in recent decades and already implemented in current germplasm banks, e.g., CIAT, Colombia; EMBRAPA, Brazil; CRI, Czech Republic; InHort, Poland; NARO, NIAS, NCSS, Japan; and many others [ 9 ]. This method consists of the use of ultra-low temperatures (−135 °C to −196 °C) of liquid nitrogen (LN), which allows cells to maintain their viability and genetic stability.…”
Section: Cryopreservationmentioning
confidence: 99%
“…Due to all these factors, germplasm banks were created that store different plant materials, to maintain a collection of the possible genetic variations existing in the world [ 9 ]. This review aims to analyze the current strategies of biodiversity protection with particular attention focused on cryopreservation as a method of long-term storage of plant material and some of its applications made in agronomy in recent years.…”
Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.
“…Cryopreservation is an important tool for storing germplasm, developed in recent decades and already implemented in current germplasm banks, e.g., CIAT, Colombia; EMBRAPA, Brazil; CRI, Czech Republic; InHort, Poland; NARO, NIAS, NCSS, Japan; and many others [ 9 ]. This method consists of the use of ultra-low temperatures (−135 °C to −196 °C) of liquid nitrogen (LN), which allows cells to maintain their viability and genetic stability.…”
Section: Cryopreservationmentioning
confidence: 99%
“…Due to all these factors, germplasm banks were created that store different plant materials, to maintain a collection of the possible genetic variations existing in the world [ 9 ]. This review aims to analyze the current strategies of biodiversity protection with particular attention focused on cryopreservation as a method of long-term storage of plant material and some of its applications made in agronomy in recent years.…”
Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.
“…Routine cryopreservation of crop collections began only a few decades ago. Currently, about 18 genebanks have cryopreserved crop collections [13,67,81,98,109]. It is estimated that about 100,000 unique accessions of vegetatively propagated and recalcitrant seed crops potentially need long-term conservation through cryopreservation while currently only about 10,000 accessions are cryopreserved [13].…”
The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.
“…Sci. 2022, 12, 1788 2 of 14 external conditions [5]. Likewise, the possibility of studying organized tissues such as shoots, meristems, and embryos makes this technique a fundamental tool for metabolomics and transcriptomics assays [6,7].…”
Nanoencapsulation with proteoliposomes from natural membranes has been proposed as a carrier for the highly efficient delivery of mineral nutrients into plant tissues. Since Boron deficiency occurred frequently in crops, and is an element with low movement in tissues, in this work, nanoencapsulated B vs free B was applied to in vitro sweet potato plants to investigate the regulation of B transporters (aquaporins and specific transporters). Additionally, an metabolomic analysis was performed, and mineral nutrient and pigment concentrations were determined. The results showed high increases in B concentration in leaves when B was applied as encapsulated, but also Fe and Mn concentration increased. Likewise, the metabolomics study showed that single carbohydrates of these plants could be related to the energy need for increasing the expression of most NIP aquaporins (NIP1;2, NIP1;3; NIP4;1, NIP4;2, NIP5;1, NIP6;1, and NIP7) and boron transporters (BOR2, BOR4 and BOR7;1). Therefore, the results were associated with the higher mobility of encapsulated B into leaves and the stimulation of transport into cells, since after applying encapsulated B, the aforementioned NIPs and BORs increased in expression.
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