Bioballs carrying a syntrophic Rhodococcus and Mycolicibacterium consortium for simultaneous sorption and biodegradation of fuel oil in contaminated freshwater

Naloka, Kallayanee; Polrit, Duangporn; Muangchinda, Chanokporn; Thoetkiattikul, Honglada; Pinyakong, Onruthai

doi:10.1016/j.chemosphere.2021.130973

Cited by 15 publications

(7 citation statements)

References 63 publications

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“…The cultured cells (5 mL) were centrifuged at 8000 rpm and 4 °C for 10 min. Cell pellets were washed twice and suspended in phosphate urea magnesium sulfate (PUM) buffer 46 or subjected to a cell hydrophobicity test according to Naloka et al 12 . The remaining cultured cells were harvested by centrifugation and washed three times with sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Biofilm formation was examined using a slightly modified method from Naloka et al 12 . Briefly, the centrifuged cells of the preculture were diluted with each tested medium to obtain an initial cell concentration of 10 7 CFU mL −1 .…”

Section: Methodsmentioning

confidence: 99%

“…Flasks were incubated at RT and 200 rpm. The immobilized cells were enumerated daily for 5 days as described by Naloka et al 12 . The morphology of the immobilized cells was visualized by scanning electron microscopy (SEM) using a JSM-IT500HR model (JEOL Ltd., Tokyo, Japan) according to Sakdapetsiri et al 47 .…”

Section: Methodsmentioning

confidence: 99%

“…Among the genus members, R. ruber is one of the promising candidates for environmental bioremediation since it is a nonpathogenic species and ability to degrade a wide range of hydrocarbon compounds such as alkanes 9 , naphthalene 10 and crude oil 11 . In our previous study, R. ruber S103 showed good performance in the degradation of various hydrocarbon compounds, high cell hydrophobicity, biosurfactant production and biofilm formation 12 . However, the success of bioaugmentation is strongly dependent on the survival and activity of exogenously inoculated microorganisms which may be affected by various abiotic factors 13 .…”

Section: Introductionmentioning

confidence: 91%

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Physiological changes in Rhodococcus ruber S103 immobilized on biobooms using low-cost media enhance stress tolerance and crude oil-degrading activity

Naloka

Jaroonrunganan

Woratecha

et al. 2022

Sci Rep

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For economic feasibility, sugarcane molasses (0.5%, w/v) containing K2HPO4 (0.26%, w/v) and mature coconut water, low value byproducts, were used in cultivation of Rhodococcus ruber S103 for inoculum production and immobilization, respectively. Physiological changes of S103 grown in low-cost media, including cell hydrophobicity, saturated/unsaturated ratio of cellular fatty acids and biofilm formation activity, enhanced stress tolerance and crude oil biodegradation in freshwater and even under high salinity (5%, w/v). Biobooms comprised of S103 immobilized on polyurethane foam (PUF) was achieved with high biomass content (1010 colony-forming units g−1 PUF) via a scale-up process in a 5-L modified fluidized-bed bioreactor within 3 days. In a 500-L mesocosm, natural freshwater was spiked with crude oil (72 g or 667 mg g−1 dry biobooms), and a simulated wave was applied. Biobooms could remove 100% of crude oil within only 3 days and simultaneously biodegraded 60% of the adsorbed oil after 7 days when compared to boom control with indigenous bacteria. In addition, biobooms had a long shelf-life (at least 100 days) with high biodegradation activity (85.2 ± 2.3%) after storage in 10% (w/v) skimmed milk at room temperature. This study demonstrates that the low-cost production of biobooms has potential for future commercial bioremediation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

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Physiological changes in Rhodococcus ruber S103 immobilized on biobooms using low-cost media enhance stress tolerance and crude oil-degrading activity

Naloka

Jaroonrunganan

Woratecha

et al. 2022

Sci Rep

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“…Recently, Steliga et al found that the species Mycolicibacterium frederiksbergense IN53 is capable of effectively degrading petroleum pollutants [5]. Two strains, Mycolicibacterium parafortuitum J101 and Mycolicibacterium austroafricanum Y502, have been isolated from sediments contaminated with PAHs- and are able to utilize various PAHs and aliphatic compounds as sole carbon sources [7]. The species Mycolicibacterium chlorophenolicum was first obtained from chlorophenol-contaminated sediments and can degrade polychlorinated phenol compounds [8].…”

Section: Introductionmentioning

confidence: 99%

Mycolicibacterium aurantiacum sp. nov. and Mycolicibacterium xanthum sp. nov., two novel actinobacteria isolated from mangrove sediments

Pan

Huang

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Two novel actinobacteria with the ability to degrade kerosene, designated as B3033T and Y57T, were isolated from mangrove sediments in Tieshan Harbour, South China Sea. Both strains are Gram-staining-positive, non-spore forming, slow-growing, oxidase-positive, non-motile and aerobic. Their major cellular fatty acids were C16 : 0 and C18 : 1ω9c. Analysis of 16S rRNA gene sequences revealed the close relationship of strain B3033T to Mycobacterium kyogaense DSM 107316T (99.4 % nucleotide identity) and strain Y57T to Mycolicibacterium chubuense ATCC 27278T (98.7 %) and Mycolicibacterium rufum JS14T (98.7 %). Whole genome average nucleotide blast identity (ANI) and the digital DNA–DNA hybridization (dDDH) values between the two isolates and the type strains of species of the genus Mycolicibacterium were lower than 94 and 45 %, respectively, which were below the threshold values of 95 % (for ANI) and 70 % (for dDDH) recommended for bacterial species differentiation. The genome sequence of B3033T comprised a circular 11.0 Mb chromosome with a DNA G+C content of 68.1 mol%. Y57T had a genome size of 5.6 Mb and a DNA G+C content of 68.7 mol%. Genes involved in degradation of aromatic compounds and copper resistance were identified in the genomes of both strains that could improve their adaptive capacity to the mangrove environment. These results combined with those of chemotaxonomic analyses, MALDI-TOF MS profiles and phenotypic analyses support the affiliation of these strains to two novel species within the genus Mycolicibacterium , for which we propose the names Mycolicibacterium aurantiacum sp. nov. B3033T (=KCTC 49712T=MCCC 1K04526T) and Mycolicibacterium xanthum sp. nov. Y57T (=KCTC 49711T=MCCC 1K04875T) as type strains.

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Biodegradation of N-hexadecane using bacterial consortium isolated from seawater contaminated with petroleum

Noveiri

Nozari

Almasi

et al. 2023

Biomass Conv. Bioref.

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Bioballs carrying a syntrophic Rhodococcus and Mycolicibacterium consortium for simultaneous sorption and biodegradation of fuel oil in contaminated freshwater

Cited by 15 publications

References 63 publications

Physiological changes in Rhodococcus ruber S103 immobilized on biobooms using low-cost media enhance stress tolerance and crude oil-degrading activity

Physiological changes in Rhodococcus ruber S103 immobilized on biobooms using low-cost media enhance stress tolerance and crude oil-degrading activity

Mycolicibacterium aurantiacum sp. nov. and Mycolicibacterium xanthum sp. nov., two novel actinobacteria isolated from mangrove sediments

Biodegradation of N-hexadecane using bacterial consortium isolated from seawater contaminated with petroleum

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