Bioavailability Study of Cefepime After Intravenous and Intramuscular Administration in Normal Broiler Chickens

Abstract

The bioavailability of cefepime in normal broiler chickens was investigated after single intramuscular and intravenous administrations at a dose of 100 mg/kg b.wt. Serum concentrations of cefepime were determined by using high performance liquid chromatography (HPLC). Following compartmental analysis, a two-compartment open model best described the concentration-time data of cefepime after intramuscular and intravenous administration. After intramuscular administration, the drug reached its maximum serum concentrations (Cmax) of 193.06 ± 2.27 μg/ml at maximum time (Tmax) of 1.138 ± 0.012 h, absorption half-life (t1/2ab) was 0.491 ± 0.027 h and (AUC0-t) was 1127.58 ± 14.48 μg/ml/h. Following a single intravenous injection, the drug was detected till 24 hours, distribution half-life (t1/2α) was 0.217 ± 0.036 h, elimination half-life (t1/2β) was of 4.608 ± 0.145 h and clearance (CL) was 0.090 ± 0.002 (mg/kg)/(μg/ml)/h, volume of distribution at steady state (Vdss) was 0.586 ± 0.11 (mg/kg)/(μg/ml) and bioavailability was 104.30 ± 2.34 %. Limits of detection and quantification were 0.03 and 0.10 µg/ml, respectively.