Bioavailability of trace metals to aquatic microorganisms: importance of chemical, biological and physical processes on biouptake

Worms, Isabelle; Simon, Dana F.; Hassler, Christel; Wilkinson, Kevin J.

doi:10.1016/j.biochi.2006.09.008

Cited by 222 publications

(166 citation statements)

References 148 publications

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“…Although not only the free cobalt species can be transported into a microbial cell (e.g. vitamin B 12 [8], Co-citrate [23] and siderophores [45] are directly taken up by the methanogenic cell), similar IC 50 values for all three measurement sets (standard deviation of only 22%) were obtained when taking only the free cobalt species (Co 2? ) concentration into account (Table 5).…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 74%

“…concentration into account (Table 5). This is due to the fact that when equilibrium conditions are established, metal toxicity can be predicted based on any metal species [45]. Although the IC 50 values for the dissolved cobalt fraction obtained for sets I and III are similar, the value from set II (Co-EDTA) is more than ten times higher (Table 5, Fig.…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 97%

“…This is due to the fact that most authors report the total cobalt concentration in the liquid phase, not considering cobalt speciation. Therefore, the observed toxic concentrations are affected by differences in medium composition, which causes changes in metal speciation [45].…”

Section: Introductionmentioning

confidence: 99%

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Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Bartacek

Fermoso

Baldó-Urrutia

et al. 2008

J Ind Microbiol Biotechnol

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The influence of cobalt speciation on the toxicity of cobalt to methylotrophic methanogenesis in anaerobic granular sludge was investigated. The cobalt speciation was studied with three different media that contained varying concentrations of complexing ligands [carbonates, phosphates and ethylenediaminetetraacetic acid (EDTA)]. Three fractions (nominal added, dissolved and free) of cobalt were determined in the liquid media and were correlated with data from batch toxicity experiments. The average concentration of cobalt that was required for 50% inhibition of methanogenic activity (IC 50 ) for free Co 2? in the three sets of measurements was 13 lmol/L with a standard deviation of 22% and a similarity of 72% between the data obtained in the three different media for the range of cobalt concentrations investigated. The standard deviation of the IC 50 for the other two fractions was much higher, i.e. 85 and 144% for the added cobalt and dissolved cobalt, respectively, and the similarity was almost 0% for both fractions. Complexation (and precipitation) with EDTA, phosphates and carbonates was shown to decrease the toxicity of cobalt on methylotrophic methanogenesis. The free cobalt concentration is proposed to be the key parameter to correlate with cobalt toxicity. Thus, the toxicity of cobalt to granular sludge can be estimated based on the equilibrium-free cobalt concentration.

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Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 74%

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 97%

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Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Bartacek

Fermoso

Baldó-Urrutia

et al. 2008

J Ind Microbiol Biotechnol

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“…10,25,[29][30][31][32] These mechanisms may be extracellular processes, such as physical exclusion due to reduced membrane permeability 31 or extracellular binding of metal ions to cell exudates or ligands. 31,33,34 Intracellular mechanisms may include the immobilisation of metals through the high affinity binding of Cu to sulfate in granules, polyphosphate bodies or calcium calcretions, or in non-metabolically active areas of the cell, e.g.…”

Section: Discussionmentioning

confidence: 99%

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Adams

Dillon

Vogt

et al. 2016

Environ. Sci. Technol.

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Metal toxicity to aquatic organisms depends on the speciation of the metal and its binding to the critical receptor site(s) (biotic ligand) of the organism. The intracellular nature of the biotic ligand for Cu in microalgal cells was investigated using the high elemental sensitivity of microprobe synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption near-edge spectroscopy (XANES). The marine microalgae, Ceratoneis closterium, Phaeodactylum tricornutum, and Tetraselmis sp. were selected based on their varying sensitivities to Cu (72-h 50% population growth inhibitions of 8–47 μg Cu/L). Intracellular Cu in control cells was similar for all three species (2.5–3.2 × 10–15 g Cu/cell) and increased 4-fold in C. closterium and Tetraselmis sp. when exposed to copper, but was unchanged in P. tricornutum (72-h exposure to 19, 40, and 40 μg Cu/L, respectively). Whole cell microprobe SR-XRF identified endogenous Cu in the central compartment (cytoplasm) of control (unexposed) cells. After Cu exposure, Cu was colocated with organelles/granules dense in P, S, Ca, and Si and this was clearly evident in thin sections of Tetraselmis sp. XANES indicated coexistence of Cu(I) and Cu(II) in control and Cu-exposed cells, with the Cu ligand (e.g., phytochelatin) in P. tricornutum different from that in C. closterium and Tetraselmis sp. This study supports the hypothesis that Cu(II) is reduced to Cu(I) and that polyphosphate bodies and phytochelatins play a significant role in the internalization and detoxification of Cu in marine microalgae.

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“…The water-soluble metal represented the most free and bioavailable relevant metal fraction (Giller et al 2009;Peijnenburg and Jager 2003;Worms et al 2006). However, direct contact assay of biosensor with soil particles could reflect a distinctly higher level of bioavailable Cd (20-or 115-fold), Hg (30-fold), and Pb (40-fold) than soil-water extract assay (Ivask et al 2002(Ivask et al , 2004.…”

Section: Application Of Biosensor To Assess the Bioavailable As In DImentioning

confidence: 99%

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

Hou

et al. 2014

Appl Microbiol Biotechnol

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An Escherichia coli arsRp::luc-based biosensor was constructed to measure the bioavailability of arsenic (As) in soil. In previous induction experiments, it produced a linear response (R 2 =0.96, P<0.01) to As from 0.05 to 5 μmol/L after a 2-h incubation. Then, both chemical sequential extraction, Community Bureau of Reference recommended sequential extraction procedures (BCR-SEPs) and E. coli biosensor, were employed to assess the impact of different long-term fertilization regimes containing N, NP, NPK, M (manure), and NPK+ M treatments on the bioavailability of arsenic (As) in soil. Per the BCR-SEPs analysis, the application of M and M+NPK led to a significant (P<0.01) increase of exchangeable As (2-7 times and 2-5 times, respectively) and reducible As (1.5-2.5 times and 1.5-2.3 times, respectively) compared with the no fertilization treated soil (CK). In addition, direct contact assay of E. coli biosensor with soil particles also supported that bioavailable As in manure-fertilized (M and M+NPK) soil was significantly higher (P<0.01) than that in CK soil (7 and 9 times, respectively). Organic carbon may be the major factor governing the increase of bioavailable As. More significantly, E. coli biosensor-determined As was only 18.46-85.17 % of exchangeable As and 20.68-90.1 % of reducible As based on BCR-SEPs. In conclusion, NKP fertilization was recommended as a more suitable regime in As-polluted soil especially with high As concentration, and this E. coli arsRp::luc-based biosensor was a more realistic approach in assessing the bioavailability of As in soil since it would not overrate the risk of As to the environment.

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Bioavailability of trace metals to aquatic microorganisms: importance of chemical, biological and physical processes on biouptake

Cited by 222 publications

References 148 publications

Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

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