Bioavailability of Reduced Coenzyme Q10 (Ubiquinol-10) in Burn Patients

Kuriyama, Naohide; Nakamura, Tomoyuki; Nakazawa, Harumasa; Wen, Tyler; Bittner, Edward A.; Goverman, Jeremy; Kaneki, Masao

doi:10.3390/metabo12070613

Cited by 4 publications

(1 citation statement)

References 75 publications

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“…Reduced CoQ 10 (Fig. 1 ) is the main form present in the body, accounting for more than 90% of the total CoQ 10 in the human circulation [ 4 , 5 ]. The reduced CoQ 10 releases electrons during the electron transfer process, and the electrons are transferred through the respiratory chain to eventually produce ATP to provide energy for the body.…”

Section: Introductionmentioning

confidence: 99%

Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators

Xiao,

Zheng,

Zhou

et al. 2023

Microb Cell Fact

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Coenzyme Q10 (CoQ10) is crucial for human beings, especially in the fields of biology and medicine. The aim of this experiment was to investigate the conditions for increasing CoQ10 production. At present, microbial fermentation is the main production method of CoQ10, and the production process of microbial CoQ10 metabolism control fermentation is very critical. Metabolic flux is one of the most important determinants of cell physiology in metabolic engineering. Metabolic flux analysis (MFA) is used to estimate the intracellular flux in metabolic networks. In this experiment, Rhodobacter sphaeroides was used as the research object to analyze the effects of aqueous ammonia (NH3·H2O) and calcium carbonate (CaCO3) on the metabolic flux of CoQ10. When CaCO3 was used to adjust the pH, the yield of CoQ10 was 274.43 mg·L−1 (8.71 mg·g−1 DCW), which was higher than that of NH3·H2O adjustment. The results indicated that when CaCO3 was used to adjust pH, more glucose-6-phosphate (G6P) entered the pentose phosphate (HMP) pathway and produced more NADPH, which enhanced the synthesis of CoQ10. At the chorismic acid node, more metabolic fluxes were involved in the synthesis of p-hydroxybenzoic acid (pHBA; the synthetic precursor of CoQ10), enhancing the anabolic flow of CoQ10. In addition, Ca2+ produced by the reaction of CaCO3 with organic acids promotes the synthesis of CoQ10. In summary, the use of CaCO3 adjustment is more favorable for the synthesis of CoQ10 by R. sphaeroides than NH3·H2O adjustment. The migration of metabolic flux caused by the perturbation of culture conditions was analyzed to compare the changes in the distribution of intracellular metabolic fluxes for the synthesis of CoQ10. Thus, the main nodes of the metabolic network were identified as G6P and chorismic acid. This provides a theoretical basis for the modification of genes related to the CoQ10 synthesis pathway.

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Section: Introductionmentioning

confidence: 99%

Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators

Xiao,

Zheng,

Zhou

et al. 2023

Microb Cell Fact

View full text Add to dashboard Cite

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Pathogenetic Mechanisms of Burn Pathology Associated with Oxidative Membrane Damage and Methods of their Correction

Vilyanen,

Pashkevich,

Borisova-Mubarakshina

et al. 2023

BIOPHYSICS

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Coenzyme Q10 supplementation in burn patients: a double-blind placebo-controlled randomized clinical trial

Kiani,

Khorsand,

Beigi

et al. 2024

Trials

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Background Burn injuries are important medical problems that, aside from skin damage, cause a systemic response including inflammation, oxidative stress, endocrine disorders, immune response, and hypermetabolic and catabolic responses which affect all the organs in the body. The aim of this study was to determine the effect of coenzyme Q10 (CoQ10) supplementation on inflammation, oxidative stress, and clinical outcomes in burn patients. Methods In a double-blind placebo-controlled randomized clinical trial, 60 burn patients were randomly assigned to receive 100 mg CoQ10 three times a day (total 300 mg/day) or a placebo for 10 days. Inflammatory markers including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), oxidative stress markers including total antioxidant capacity (TAC), malondialdehyde (MDA) and superoxide dismutase (SOD) activity, fasting blood glucose (FBG), blood urea nitrogen (BUN), creatinine, white blood cells (WBC), and body temperature were assessed as primary outcomes and albumin, prothrombin time (PT), partial thromboplastin time (PTT), international normalized ratio (INR), other hematological parameters, blood pressure, O2 saturation, ICU duration, and 28-mortality rate were assessed as secondary outcomes. Results Fifty-two participants completed the trial. CRP and ESR levels were not significantly different between CoQ10 and placebo groups at the end of the study (P = 0.550 and P = 0.306, respectively). No significant differences between groups were observed for TAC (P = 0.865), MDA (P = 0.692), and SOD activity (P = 0.633) as well. Administration of CoQ10 resulted in a significant increase in albumin levels compared to placebo (P = 0.031). There was no statistically significant difference between the two groups in other measured outcomes (P > 0.05). Conclusion Results showed that in patients with burn injury, CoQ10 administration had no effect on inflammatory markers and oxidative stress, although serum albumin levels were improved after supplementation. Further studies with albumin as the primary outcome are needed to confirm this finding.

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Bioavailability of Reduced Coenzyme Q10 (Ubiquinol-10) in Burn Patients

Cited by 4 publications

References 75 publications

Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators

Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators

Pathogenetic Mechanisms of Burn Pathology Associated with Oxidative Membrane Damage and Methods of their Correction

Coenzyme Q10 supplementation in burn patients: a double-blind placebo-controlled randomized clinical trial

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