Bioavailability of dietary sodium copper chlorophyllin and its effect on antioxidant defence parameters of Wistar rats

Abstract

BACKGROUND: There has been growing interest in sodium copper chlorophyllin (Cu-Chl) as a food colourant and supplement owing to its beneficial biological activities. Studies have revealed that this green pigment inhibits experimental carcinogenesis and interacts with proteins and genotoxic agents. Health-related activities have also been associated with the prevention of lipid peroxidation. However, intestinal absorption of this pigment has been considered insignificant, raising questions of whether eventual biological properties are related to pre-or post-absorptive actions. In this study, intestinal absorption of Cu-Chl and its appearance in serum and organs were estimated by high-performance liquid chromatography analysis in rat feeding experiments. The effect of ingested Cu-Chl on lipid peroxidation was analysed by measuring thiobarbituric acid-reactive substances and antioxidant enzyme activities in hepatic and brain tissues of oxidative stress-induced rats.

Chemistry in the Bioactivity of Chlorophylls: An Overview

“…5), but they behave different in the digestive tract, so that only Cu-chlorin e 4 is found in serum, liver and kidneys of rats [58] or in humans" sera [57]. The study of Gomes et al [58] evaluated the effects on the antioxidant defense system of liver and brain of rats of the absorbed chlorophyllin. The results show that presence of chlorin protected brain lipids against peroxidation observed under vitamin E deficiency.…”

Sodium Copper Chlorophyllin Immobilization onto Hippospongia communis Marine Demosponge Skeleton and Its Antibacterial Activity

“…The antimutagenic activity of chlorophyllin comes also from the scavenging of free radicals and active oxygen species, and suppression of or interference with metabolic activation by a specific cytochrome (P-450) and other metabolizing enzymes [20,21]. Other bioactivities are also attributed to chlorophyllin, such as immunomodulatory and antiapoptotic effects [22], antioxidant activity against oxidative stress or radiation-generated reactive oxygen species [23,24], and antibacterial effects [5,25,26,27]. SCC is used as a dietary supplement, in food, drugs and cosmetics [28], in textile dye [1,29], as an internal deodorizer [30] and as a natural wound healer [31].…”

Copper chlorophyllin: A food colorant with bioactive properties?

“…The Cu-Chl absorption and distribution to different tissues were also investigated in rats by Gomes et al (2009), who observed the absence of copper chlorin e 6 in blood serum and tissues (liver and kidney), possibly due to its non-absorption, its degradation or its interaction with other dietary components. However, they reported the presence of copper chlorin e 4 in both serum and tissue extracts (liver and kidney).…”